Potential role for imidazole in the rhythmic respiratory activity of the in vitro neonatal rat brainstem
We used the imidazole-binding agent, diethylpyrocarbonate (DEPC), to test the hypothesis that rhythmic respiratory activity of the in vitro neonatal rat brainstem-spinal cord preparation was functionally dependent on imidazole. Neural activity was recorded from spinal nerves (C1-C4) during superfusi...
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| Veröffentlicht in: | Neuroscience letters 1998-07, Vol.251 (3), p.153-156 |
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| creator | Krause, William L Kazemi, Homayoun Burton, Melvin D |
| description | We used the imidazole-binding agent, diethylpyrocarbonate (DEPC), to test the hypothesis that rhythmic respiratory activity of the in vitro neonatal rat brainstem-spinal cord preparation was functionally dependent on imidazole. Neural activity was recorded from spinal nerves (C1-C4) during superfusion with 95%O
2/5%CO
2 buffer at pH 7.3 and T=26°C. Superfusate containing DEPC (40 mM) caused cessation of rhythmic activity within minutes. In eight of 33 preparations, microinjection of DEPC (32 nmol) onto the ventral medullary surface (VMS) reduced burst amplitude by at least 50% within 10 min, and in 12 of 33 preparations, microinjection of DEPC produced neural apnea. Therefore, we conclude that proteins containing imidazole near the VMS are critically important for the maintenance of rhythmic respiratory activity in vitro. Furthermore, alphastat regulation of respiration may be an essential trait of this preparation. |
| doi_str_mv | 10.1016/S0304-3940(98)00502-3 |
| format | Article |
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2/5%CO
2 buffer at pH 7.3 and T=26°C. Superfusate containing DEPC (40 mM) caused cessation of rhythmic activity within minutes. In eight of 33 preparations, microinjection of DEPC (32 nmol) onto the ventral medullary surface (VMS) reduced burst amplitude by at least 50% within 10 min, and in 12 of 33 preparations, microinjection of DEPC produced neural apnea. Therefore, we conclude that proteins containing imidazole near the VMS are critically important for the maintenance of rhythmic respiratory activity in vitro. Furthermore, alphastat regulation of respiration may be an essential trait of this preparation.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/S0304-3940(98)00502-3</identifier><identifier>PMID: 9726366</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Alphastat hypothesis ; Animals ; Animals, Newborn ; Biological and medical sciences ; Brain Stem - drug effects ; Brain Stem - physiology ; Control of breathing ; Diethyl Pyrocarbonate - metabolism ; Diethyl Pyrocarbonate - pharmacology ; Diethylpyrocarbonate ; Fundamental and applied biological sciences. Psychology ; Histidine - physiology ; Hydroxylamine - pharmacology ; Imidazole-histidine ; Imidazoles - antagonists & inhibitors ; Imidazoles - metabolism ; In Vitro Techniques ; Medulla Oblongata - drug effects ; Microinjections ; Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration ; Periodicity ; Rats ; Rats, Sprague-Dawley ; Respiration - drug effects ; Spinal Cord - drug effects ; Spinal Cord - physiology ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience letters, 1998-07, Vol.251 (3), p.153-156</ispartof><rights>1998 Elsevier Science Ireland Ltd</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-77fe7f04aa816a5d5dd38ee3790d8bd2484e7b876b1b102374d1c1f6608a92af3</citedby><cites>FETCH-LOGICAL-c420t-77fe7f04aa816a5d5dd38ee3790d8bd2484e7b876b1b102374d1c1f6608a92af3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0304-3940(98)00502-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2367967$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9726366$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Krause, William L</creatorcontrib><creatorcontrib>Kazemi, Homayoun</creatorcontrib><creatorcontrib>Burton, Melvin D</creatorcontrib><title>Potential role for imidazole in the rhythmic respiratory activity of the in vitro neonatal rat brainstem</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>We used the imidazole-binding agent, diethylpyrocarbonate (DEPC), to test the hypothesis that rhythmic respiratory activity of the in vitro neonatal rat brainstem-spinal cord preparation was functionally dependent on imidazole. Neural activity was recorded from spinal nerves (C1-C4) during superfusion with 95%O
2/5%CO
2 buffer at pH 7.3 and T=26°C. Superfusate containing DEPC (40 mM) caused cessation of rhythmic activity within minutes. In eight of 33 preparations, microinjection of DEPC (32 nmol) onto the ventral medullary surface (VMS) reduced burst amplitude by at least 50% within 10 min, and in 12 of 33 preparations, microinjection of DEPC produced neural apnea. Therefore, we conclude that proteins containing imidazole near the VMS are critically important for the maintenance of rhythmic respiratory activity in vitro. Furthermore, alphastat regulation of respiration may be an essential trait of this preparation.</description><subject>Alphastat hypothesis</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Biological and medical sciences</subject><subject>Brain Stem - drug effects</subject><subject>Brain Stem - physiology</subject><subject>Control of breathing</subject><subject>Diethyl Pyrocarbonate - metabolism</subject><subject>Diethyl Pyrocarbonate - pharmacology</subject><subject>Diethylpyrocarbonate</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Histidine - physiology</subject><subject>Hydroxylamine - pharmacology</subject><subject>Imidazole-histidine</subject><subject>Imidazoles - antagonists & inhibitors</subject><subject>Imidazoles - metabolism</subject><subject>In Vitro Techniques</subject><subject>Medulla Oblongata - drug effects</subject><subject>Microinjections</subject><subject>Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration</subject><subject>Periodicity</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Respiration - drug effects</subject><subject>Spinal Cord - drug effects</subject><subject>Spinal Cord - physiology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUlrHDEQhUWIcSZOfoJBhxCSQydSS63lFIzJBgYbkpyFWioxCt2tiaQxTH69NQtz9ako3lcL7yF0TcknSqj4_IswwjumOfmg1UdCBtJ37AVaUSX7TmrZv0SrM_IKvS7lL2kUHfglumyyYEKs0PohVVhqtBPOaQIcUsZxjt7-33dxwXUNOK93dT1HhzOUTcy2przD1tX4GOsOp3CAGtvanPACabF1v9BWPGYbl1JhfoMugp0KvD3VK_Tn29fftz-6u_vvP29v7jrHe1I7KQPIQLi1igo7-MF7pgCY1MSr0fdccZCjkmKkIyU9k9xTR4MQRFnd28Cu0Pvj3k1O_7ZQqpljcTBNtv21LUYyTYae6GdBKikXg6YNHI6gy6mUDMFscpxt3hlKzD4Kc4jC7H02WplDFIa1uevTge04gz9Pnbxv-ruTbouzU8h2cbGcsZ4JqYVs2JcjBs21xwjZFBdhceBjBleNT_GZR54Ag5-mZg</recordid><startdate>19980731</startdate><enddate>19980731</enddate><creator>Krause, William L</creator><creator>Kazemi, Homayoun</creator><creator>Burton, Melvin D</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19980731</creationdate><title>Potential role for imidazole in the rhythmic respiratory activity of the in vitro neonatal rat brainstem</title><author>Krause, William L ; Kazemi, Homayoun ; Burton, Melvin D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-77fe7f04aa816a5d5dd38ee3790d8bd2484e7b876b1b102374d1c1f6608a92af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Alphastat hypothesis</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Biological and medical sciences</topic><topic>Brain Stem - drug effects</topic><topic>Brain Stem - physiology</topic><topic>Control of breathing</topic><topic>Diethyl Pyrocarbonate - metabolism</topic><topic>Diethyl Pyrocarbonate - pharmacology</topic><topic>Diethylpyrocarbonate</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Histidine - physiology</topic><topic>Hydroxylamine - pharmacology</topic><topic>Imidazole-histidine</topic><topic>Imidazoles - antagonists & inhibitors</topic><topic>Imidazoles - metabolism</topic><topic>In Vitro Techniques</topic><topic>Medulla Oblongata - drug effects</topic><topic>Microinjections</topic><topic>Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration</topic><topic>Periodicity</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Respiration - drug effects</topic><topic>Spinal Cord - drug effects</topic><topic>Spinal Cord - physiology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krause, William L</creatorcontrib><creatorcontrib>Kazemi, Homayoun</creatorcontrib><creatorcontrib>Burton, Melvin D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krause, William L</au><au>Kazemi, Homayoun</au><au>Burton, Melvin D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Potential role for imidazole in the rhythmic respiratory activity of the in vitro neonatal rat brainstem</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1998-07-31</date><risdate>1998</risdate><volume>251</volume><issue>3</issue><spage>153</spage><epage>156</epage><pages>153-156</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>We used the imidazole-binding agent, diethylpyrocarbonate (DEPC), to test the hypothesis that rhythmic respiratory activity of the in vitro neonatal rat brainstem-spinal cord preparation was functionally dependent on imidazole. Neural activity was recorded from spinal nerves (C1-C4) during superfusion with 95%O
2/5%CO
2 buffer at pH 7.3 and T=26°C. Superfusate containing DEPC (40 mM) caused cessation of rhythmic activity within minutes. In eight of 33 preparations, microinjection of DEPC (32 nmol) onto the ventral medullary surface (VMS) reduced burst amplitude by at least 50% within 10 min, and in 12 of 33 preparations, microinjection of DEPC produced neural apnea. Therefore, we conclude that proteins containing imidazole near the VMS are critically important for the maintenance of rhythmic respiratory activity in vitro. Furthermore, alphastat regulation of respiration may be an essential trait of this preparation.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>9726366</pmid><doi>10.1016/S0304-3940(98)00502-3</doi><tpages>4</tpages></addata></record> |
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| subjects | Alphastat hypothesis Animals Animals, Newborn Biological and medical sciences Brain Stem - drug effects Brain Stem - physiology Control of breathing Diethyl Pyrocarbonate - metabolism Diethyl Pyrocarbonate - pharmacology Diethylpyrocarbonate Fundamental and applied biological sciences. Psychology Histidine - physiology Hydroxylamine - pharmacology Imidazole-histidine Imidazoles - antagonists & inhibitors Imidazoles - metabolism In Vitro Techniques Medulla Oblongata - drug effects Microinjections Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration Periodicity Rats Rats, Sprague-Dawley Respiration - drug effects Spinal Cord - drug effects Spinal Cord - physiology Vertebrates: nervous system and sense organs |
| title | Potential role for imidazole in the rhythmic respiratory activity of the in vitro neonatal rat brainstem |
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