Purification and Characterization of a Polysome-associated Endoribonuclease That Degrades c-myc mRNA in Vitro
The regulation of mRNA half-lives is determined by multiple factors, including the activity of the messenger RNases (mRNases) responsible for destroying mRNA molecules. Previously, we used cell-free mRNA decay assays to identify a polysome-associated endonuclease that cleaves c-mycmRNA within the co...
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| Veröffentlicht in: | The Journal of biological chemistry 1998-09, Vol.273 (39), p.25261-25271 |
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| container_title | The Journal of biological chemistry |
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| creator | Lee, Chow Hwee Leeds, Peter Ross, Jeffrey |
| description | The regulation of mRNA half-lives is determined by multiple factors, including the activity of the messenger RNases (mRNases) responsible for destroying mRNA molecules. Previously, we used cell-free mRNA decay assays to identify a polysome-associated endonuclease that cleaves c-mycmRNA within the coding region. A similar activity has been solubilized and partially purified from a high salt extract of adult rat liver polysomes. Based on a correlation between protein and enzyme activity, the endonuclease is tentatively identified as a ∼39-kDa protein. It cleaves the coding region stability determinant of c-myc mRNA with considerable specificity. Cleavages occur predominantly in an A-rich segment of the RNA. The endonuclease is resistant to RNase A inhibitors, sensitive to vanadyl ribonucleoside complex, and dependent on magnesium. In these and other respects, the soluble enzyme we have purified resembles the polysome-associated c-myc mRNase. |
| doi_str_mv | 10.1074/jbc.273.39.25261 |
| format | Article |
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In these and other respects, the soluble enzyme we have purified resembles the polysome-associated c-myc mRNase.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.273.39.25261</identifier><identifier>PMID: 9737991</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Base Sequence ; Binding Sites ; DNA Primers ; DNA, Complementary ; Endoribonucleases - isolation & purification ; Endoribonucleases - metabolism ; Hydrolysis ; Liver - enzymology ; Male ; Molecular Sequence Data ; Polyribosomes - metabolism ; Proto-Oncogene Proteins c-myc - genetics ; Rats ; Rats, Sprague-Dawley ; RNA, Messenger - metabolism</subject><ispartof>The Journal of biological chemistry, 1998-09, Vol.273 (39), p.25261-25271</ispartof><rights>1998 © 1998 ASBMB. 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Previously, we used cell-free mRNA decay assays to identify a polysome-associated endonuclease that cleaves c-mycmRNA within the coding region. A similar activity has been solubilized and partially purified from a high salt extract of adult rat liver polysomes. Based on a correlation between protein and enzyme activity, the endonuclease is tentatively identified as a ∼39-kDa protein. It cleaves the coding region stability determinant of c-myc mRNA with considerable specificity. Cleavages occur predominantly in an A-rich segment of the RNA. The endonuclease is resistant to RNase A inhibitors, sensitive to vanadyl ribonucleoside complex, and dependent on magnesium. In these and other respects, the soluble enzyme we have purified resembles the polysome-associated c-myc mRNase.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>DNA Primers</subject><subject>DNA, Complementary</subject><subject>Endoribonucleases - isolation & purification</subject><subject>Endoribonucleases - metabolism</subject><subject>Hydrolysis</subject><subject>Liver - enzymology</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>Polyribosomes - metabolism</subject><subject>Proto-Oncogene Proteins c-myc - genetics</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA, Messenger - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUUtv1DAQthBVWQp3Lkg-IG7Z2rETx9yqpTykCipUEDfLGU8aV0nc2glo--sxZNUDEmIuI8330Mx8hLzgbMuZkqc3LWxLJbZCb8uqrPkjsuGsEYWo-PfHZMNYyQtdVs0T8jSlG5ZLan5MjrUSSmu-IePlEn3nwc4-TNROju56Gy3MGP39OgwdtfQyDPsURixsSgG8ndHR88mF6NswLTCgTUivejvTt3gdrcNEoRj3QMcvn86on-g3P8fwjBx1dkj4_NBPyNd351e7D8XF5_cfd2cXBUip5qKqmWxqaEvdIJbYSNkyBdKC6ISSGruGWWaVqwXjpXMVq0XTVdKi7RpXORAn5PXqexvD3YJpNqNPgMNgJwxLMko0-XpV_5fIFdNSC5WJbCVCDClF7Mxt9KONe8OZ-R2FyVGYHIUR2vyJIkteHryXdkT3IDj8PuOvVrz31_1PH9G0PkCP4982b1Ya5of98BhNAo8ToMsSmI0L_t87_AIEMaT6</recordid><startdate>19980925</startdate><enddate>19980925</enddate><creator>Lee, Chow Hwee</creator><creator>Leeds, Peter</creator><creator>Ross, Jeffrey</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19980925</creationdate><title>Purification and Characterization of a Polysome-associated Endoribonuclease That Degrades c-myc mRNA in Vitro</title><author>Lee, Chow Hwee ; Leeds, Peter ; Ross, Jeffrey</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-560486cb298ee2e844b07c4ac3f3749ef80a0a7d63012dd50638f54aeaf8d5dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>DNA Primers</topic><topic>DNA, Complementary</topic><topic>Endoribonucleases - isolation & purification</topic><topic>Endoribonucleases - metabolism</topic><topic>Hydrolysis</topic><topic>Liver - enzymology</topic><topic>Male</topic><topic>Molecular Sequence Data</topic><topic>Polyribosomes - metabolism</topic><topic>Proto-Oncogene Proteins c-myc - genetics</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Chow Hwee</creatorcontrib><creatorcontrib>Leeds, Peter</creatorcontrib><creatorcontrib>Ross, Jeffrey</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Chow Hwee</au><au>Leeds, Peter</au><au>Ross, Jeffrey</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification and Characterization of a Polysome-associated Endoribonuclease That Degrades c-myc mRNA in Vitro</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1998-09-25</date><risdate>1998</risdate><volume>273</volume><issue>39</issue><spage>25261</spage><epage>25271</epage><pages>25261-25271</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>The regulation of mRNA half-lives is determined by multiple factors, including the activity of the messenger RNases (mRNases) responsible for destroying mRNA molecules. 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| ispartof | The Journal of biological chemistry, 1998-09, Vol.273 (39), p.25261-25271 |
| issn | 0021-9258 1083-351X |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Base Sequence Binding Sites DNA Primers DNA, Complementary Endoribonucleases - isolation & purification Endoribonucleases - metabolism Hydrolysis Liver - enzymology Male Molecular Sequence Data Polyribosomes - metabolism Proto-Oncogene Proteins c-myc - genetics Rats Rats, Sprague-Dawley RNA, Messenger - metabolism |
| title | Purification and Characterization of a Polysome-associated Endoribonuclease That Degrades c-myc mRNA in Vitro |
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