Binding of rat brain hexokinase to recombinant yeast mitochondria: effect of environmental factors and the source of porin
Heterologous binding of rat brain hexokinase to wild type, porinless, and recombinant yeast mitochondria expressing human porin was assessed, partially characterized, and compared to that in the homologous system (rat liver mitochondria). With porin-containing yeast mitochondria it is shown that (i)...
Gespeichert in:
| Veröffentlicht in: | Journal of bioenergetics and biomembranes 1998-06, Vol.30 (3), p.245-255 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 255 |
|---|---|
| container_issue | 3 |
| container_start_page | 245 |
| container_title | Journal of bioenergetics and biomembranes |
| container_volume | 30 |
| creator | Aflalo, C Azoulay, H |
| description | Heterologous binding of rat brain hexokinase to wild type, porinless, and recombinant yeast mitochondria expressing human porin was assessed, partially characterized, and compared to that in the homologous system (rat liver mitochondria). With porin-containing yeast mitochondria it is shown that (i) a significant, saturable association occurs; (ii) its extent and apparent affinity, correlated with the origin of porin, are enhanced in the presence of dextran; (iii) the binding requires Mg ions and apparently follows a complex cooperative mechanism. This heterologous association does not seem to differ fundamentally from that in the homologous system and represents a good basis for molecular studies in yeast. With porinless yeast mitochondria, binding occurs at much lower affinity, but to many more sites per mitochondrion. The results indicating a major but not exclusive role for porin in the binding are discussed in terms of (i) the mode and mechanism of binding, and (ii) the suitability of the rat hexokinase-yeast mitochondria couple for the study of heterogeneous catalysis in reconstituted cellular model systems. |
| doi_str_mv | 10.1023/A:1020544803475 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_73896490</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73896490</sourcerecordid><originalsourceid>FETCH-LOGICAL-c279t-91b0e38712fabca35e936e70b1ca23933a3b3d4387d175e05ee7dea6d2df0f873</originalsourceid><addsrcrecordid>eNpd0M1LxDAQBfAgyrqunj0JwYO3atJpmsabil8geFHwVtJk6ka3yZpkxfWvt-KePD0GfjweQ8ghZ6eclXB2cT4GE1XVMKik2CJTLiQUddPwbTJlvBJFJdXLLtlL6Y0x1jDBJmSiJABTfEq-L523zr_S0NOoM-2idp7O8Su8O68T0hxoRBOGbjx9pmvUKdPB5WDmwdvo9DnFvkeTfxvQf7oY_IA-6wXttckhJqq9pXmONIVVNPjrliE6v092er1IeLDJGXm-uX66uiseHm_vry4eClNKlQvFO4bQSF72ujMaBCqoUbKOG12CAtDQga1GYbkUyASitKhrW9qe9Y2EGTn5613G8LHClNvBJYOLhfYYVqmV0Ki6UmyEx__g27jYj9taKSTnZc1hREcbtOoGtO0yukHHdbv5KPwA34J5lQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>757112613</pqid></control><display><type>article</type><title>Binding of rat brain hexokinase to recombinant yeast mitochondria: effect of environmental factors and the source of porin</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Aflalo, C ; Azoulay, H</creator><creatorcontrib>Aflalo, C ; Azoulay, H</creatorcontrib><description>Heterologous binding of rat brain hexokinase to wild type, porinless, and recombinant yeast mitochondria expressing human porin was assessed, partially characterized, and compared to that in the homologous system (rat liver mitochondria). With porin-containing yeast mitochondria it is shown that (i) a significant, saturable association occurs; (ii) its extent and apparent affinity, correlated with the origin of porin, are enhanced in the presence of dextran; (iii) the binding requires Mg ions and apparently follows a complex cooperative mechanism. This heterologous association does not seem to differ fundamentally from that in the homologous system and represents a good basis for molecular studies in yeast. With porinless yeast mitochondria, binding occurs at much lower affinity, but to many more sites per mitochondrion. The results indicating a major but not exclusive role for porin in the binding are discussed in terms of (i) the mode and mechanism of binding, and (ii) the suitability of the rat hexokinase-yeast mitochondria couple for the study of heterogeneous catalysis in reconstituted cellular model systems.</description><identifier>ISSN: 0145-479X</identifier><identifier>EISSN: 1573-6881</identifier><identifier>DOI: 10.1023/A:1020544803475</identifier><identifier>PMID: 9733091</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Animals ; Brain - enzymology ; Catalysis ; Environmental effects ; Environmental factors ; Hexokinase - metabolism ; Humans ; Mitochondria ; Mitochondria - metabolism ; Porins - metabolism ; Proteins ; Rats ; Recombinant Fusion Proteins - metabolism ; Saccharomyces cerevisiae - metabolism ; Yeasts</subject><ispartof>Journal of bioenergetics and biomembranes, 1998-06, Vol.30 (3), p.245-255</ispartof><rights>Plenum Publishing Corporation 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c279t-91b0e38712fabca35e936e70b1ca23933a3b3d4387d175e05ee7dea6d2df0f873</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9733091$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aflalo, C</creatorcontrib><creatorcontrib>Azoulay, H</creatorcontrib><title>Binding of rat brain hexokinase to recombinant yeast mitochondria: effect of environmental factors and the source of porin</title><title>Journal of bioenergetics and biomembranes</title><addtitle>J Bioenerg Biomembr</addtitle><description>Heterologous binding of rat brain hexokinase to wild type, porinless, and recombinant yeast mitochondria expressing human porin was assessed, partially characterized, and compared to that in the homologous system (rat liver mitochondria). With porin-containing yeast mitochondria it is shown that (i) a significant, saturable association occurs; (ii) its extent and apparent affinity, correlated with the origin of porin, are enhanced in the presence of dextran; (iii) the binding requires Mg ions and apparently follows a complex cooperative mechanism. This heterologous association does not seem to differ fundamentally from that in the homologous system and represents a good basis for molecular studies in yeast. With porinless yeast mitochondria, binding occurs at much lower affinity, but to many more sites per mitochondrion. The results indicating a major but not exclusive role for porin in the binding are discussed in terms of (i) the mode and mechanism of binding, and (ii) the suitability of the rat hexokinase-yeast mitochondria couple for the study of heterogeneous catalysis in reconstituted cellular model systems.</description><subject>Animals</subject><subject>Brain - enzymology</subject><subject>Catalysis</subject><subject>Environmental effects</subject><subject>Environmental factors</subject><subject>Hexokinase - metabolism</subject><subject>Humans</subject><subject>Mitochondria</subject><subject>Mitochondria - metabolism</subject><subject>Porins - metabolism</subject><subject>Proteins</subject><subject>Rats</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Yeasts</subject><issn>0145-479X</issn><issn>1573-6881</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpd0M1LxDAQBfAgyrqunj0JwYO3atJpmsabil8geFHwVtJk6ka3yZpkxfWvt-KePD0GfjweQ8ghZ6eclXB2cT4GE1XVMKik2CJTLiQUddPwbTJlvBJFJdXLLtlL6Y0x1jDBJmSiJABTfEq-L523zr_S0NOoM-2idp7O8Su8O68T0hxoRBOGbjx9pmvUKdPB5WDmwdvo9DnFvkeTfxvQf7oY_IA-6wXttckhJqq9pXmONIVVNPjrliE6v092er1IeLDJGXm-uX66uiseHm_vry4eClNKlQvFO4bQSF72ujMaBCqoUbKOG12CAtDQga1GYbkUyASitKhrW9qe9Y2EGTn5613G8LHClNvBJYOLhfYYVqmV0Ki6UmyEx__g27jYj9taKSTnZc1hREcbtOoGtO0yukHHdbv5KPwA34J5lQ</recordid><startdate>19980601</startdate><enddate>19980601</enddate><creator>Aflalo, C</creator><creator>Azoulay, H</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QO</scope><scope>7QP</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>19980601</creationdate><title>Binding of rat brain hexokinase to recombinant yeast mitochondria: effect of environmental factors and the source of porin</title><author>Aflalo, C ; Azoulay, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c279t-91b0e38712fabca35e936e70b1ca23933a3b3d4387d175e05ee7dea6d2df0f873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Brain - enzymology</topic><topic>Catalysis</topic><topic>Environmental effects</topic><topic>Environmental factors</topic><topic>Hexokinase - metabolism</topic><topic>Humans</topic><topic>Mitochondria</topic><topic>Mitochondria - metabolism</topic><topic>Porins - metabolism</topic><topic>Proteins</topic><topic>Rats</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aflalo, C</creatorcontrib><creatorcontrib>Azoulay, H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of bioenergetics and biomembranes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aflalo, C</au><au>Azoulay, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Binding of rat brain hexokinase to recombinant yeast mitochondria: effect of environmental factors and the source of porin</atitle><jtitle>Journal of bioenergetics and biomembranes</jtitle><addtitle>J Bioenerg Biomembr</addtitle><date>1998-06-01</date><risdate>1998</risdate><volume>30</volume><issue>3</issue><spage>245</spage><epage>255</epage><pages>245-255</pages><issn>0145-479X</issn><eissn>1573-6881</eissn><abstract>Heterologous binding of rat brain hexokinase to wild type, porinless, and recombinant yeast mitochondria expressing human porin was assessed, partially characterized, and compared to that in the homologous system (rat liver mitochondria). With porin-containing yeast mitochondria it is shown that (i) a significant, saturable association occurs; (ii) its extent and apparent affinity, correlated with the origin of porin, are enhanced in the presence of dextran; (iii) the binding requires Mg ions and apparently follows a complex cooperative mechanism. This heterologous association does not seem to differ fundamentally from that in the homologous system and represents a good basis for molecular studies in yeast. With porinless yeast mitochondria, binding occurs at much lower affinity, but to many more sites per mitochondrion. The results indicating a major but not exclusive role for porin in the binding are discussed in terms of (i) the mode and mechanism of binding, and (ii) the suitability of the rat hexokinase-yeast mitochondria couple for the study of heterogeneous catalysis in reconstituted cellular model systems.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>9733091</pmid><doi>10.1023/A:1020544803475</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0145-479X |
| ispartof | Journal of bioenergetics and biomembranes, 1998-06, Vol.30 (3), p.245-255 |
| issn | 0145-479X 1573-6881 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73896490 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Brain - enzymology Catalysis Environmental effects Environmental factors Hexokinase - metabolism Humans Mitochondria Mitochondria - metabolism Porins - metabolism Proteins Rats Recombinant Fusion Proteins - metabolism Saccharomyces cerevisiae - metabolism Yeasts |
| title | Binding of rat brain hexokinase to recombinant yeast mitochondria: effect of environmental factors and the source of porin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T04%3A39%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Binding%20of%20rat%20brain%20hexokinase%20to%20recombinant%20yeast%20mitochondria:%20effect%20of%20environmental%20factors%20and%20the%20source%20of%20porin&rft.jtitle=Journal%20of%20bioenergetics%20and%20biomembranes&rft.au=Aflalo,%20C&rft.date=1998-06-01&rft.volume=30&rft.issue=3&rft.spage=245&rft.epage=255&rft.pages=245-255&rft.issn=0145-479X&rft.eissn=1573-6881&rft_id=info:doi/10.1023/A:1020544803475&rft_dat=%3Cproquest_pubme%3E73896490%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=757112613&rft_id=info:pmid/9733091&rfr_iscdi=true |