Physiological and molecular biological characterization of ammonia oxidation of the heterotrophic nitrifier Pseudomonas putida
The heterotrophic nitrifier Pseudomonas putida aerobically oxidized ammonia to hydroxylamine, nitrite, and nitrate. Product formation was accompanied by a small but significant release of NO, whereas N2O evolution could not be detected under the assay conditions employed. The isolate reduced nitrate...
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| Veröffentlicht in: | Current microbiology 1998-10, Vol.37 (4), p.281-288 |
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| creator | Daum, M. (Universitat zu Koln, Koln, Germany.) Zimmer, W Papen, H Kloos, K Nawrath, K Bothe, H |
| description | The heterotrophic nitrifier Pseudomonas putida aerobically oxidized ammonia to hydroxylamine, nitrite, and nitrate. Product formation was accompanied by a small but significant release of NO, whereas N2O evolution could not be detected under the assay conditions employed. The isolate reduced nitrate to nitrite and partially further to NO under anaerobic conditions. Aerobically grown cells utilized gamma-aminobutyrate as a carbon source and as a N-source by ammonification. The physiological experiments, in particular the inhibition pattern by C2H2, indicated that P. putida expressed an ammonia monooxigenase. DNA-hybridization with an amoA gene probe coding for the smaller subunit of the ammonia monooxigenase of Nitrosomonas europaea allowed us to identify, to clone, and to sequence a region with an open reading frame showing distinct sequence similarities to the amoA gene of autotrophic ammonia oxidizers |
| doi_str_mv | 10.1007/s002849900379 |
| format | Article |
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(Universitat zu Koln, Koln, Germany.) ; Zimmer, W ; Papen, H ; Kloos, K ; Nawrath, K ; Bothe, H</creator><creatorcontrib>Daum, M. (Universitat zu Koln, Koln, Germany.) ; Zimmer, W ; Papen, H ; Kloos, K ; Nawrath, K ; Bothe, H</creatorcontrib><description>The heterotrophic nitrifier Pseudomonas putida aerobically oxidized ammonia to hydroxylamine, nitrite, and nitrate. Product formation was accompanied by a small but significant release of NO, whereas N2O evolution could not be detected under the assay conditions employed. The isolate reduced nitrate to nitrite and partially further to NO under anaerobic conditions. Aerobically grown cells utilized gamma-aminobutyrate as a carbon source and as a N-source by ammonification. The physiological experiments, in particular the inhibition pattern by C2H2, indicated that P. putida expressed an ammonia monooxigenase. DNA-hybridization with an amoA gene probe coding for the smaller subunit of the ammonia monooxigenase of Nitrosomonas europaea allowed us to identify, to clone, and to sequence a region with an open reading frame showing distinct sequence similarities to the amoA gene of autotrophic ammonia oxidizers</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s002849900379</identifier><identifier>PMID: 9732537</identifier><identifier>CODEN: CUMIDD</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Aerobiosis ; ALEMANIA ; ALLEMAGNE ; Amino Acid Sequence ; Ammonia ; Ammonia - metabolism ; Ammonification ; Anaerobic conditions ; BACTERIA ; Bacteriology ; Biological and medical sciences ; Carbon sources ; Cloning, Molecular ; FLORA DEL SUELO ; FLORE DU SOL ; FOREST SOILS ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; GERMANY ; Hydroxylamine - metabolism ; Metabolism. Enzymes ; Microbiology ; Molecular Sequence Data ; Nitrates - metabolism ; NITRIFICACION ; NITRIFICATION ; Open Reading Frames ; Oxidation-Reduction ; Oxidoreductases - genetics ; Oxidoreductases - metabolism ; Physiology ; PSEUDOMONAS PUTIDA ; Pseudomonas putida - genetics ; Pseudomonas putida - growth & development ; Pseudomonas putida - metabolism ; Restriction Mapping ; Sequence Homology, Amino Acid ; SOIL FLORA ; SOL DE FORET ; SUELO FORESTAL</subject><ispartof>Current microbiology, 1998-10, Vol.37 (4), p.281-288</ispartof><rights>1998 INIST-CNRS</rights><rights>Springer-Verlag New York Inc. 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-e6e33d1cb2ec93f906d220e6342c560099bc615a892bfe13b28904fb6642ee803</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2425810$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9732537$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Daum, M. (Universitat zu Koln, Koln, Germany.)</creatorcontrib><creatorcontrib>Zimmer, W</creatorcontrib><creatorcontrib>Papen, H</creatorcontrib><creatorcontrib>Kloos, K</creatorcontrib><creatorcontrib>Nawrath, K</creatorcontrib><creatorcontrib>Bothe, H</creatorcontrib><title>Physiological and molecular biological characterization of ammonia oxidation of the heterotrophic nitrifier Pseudomonas putida</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><description>The heterotrophic nitrifier Pseudomonas putida aerobically oxidized ammonia to hydroxylamine, nitrite, and nitrate. Product formation was accompanied by a small but significant release of NO, whereas N2O evolution could not be detected under the assay conditions employed. The isolate reduced nitrate to nitrite and partially further to NO under anaerobic conditions. Aerobically grown cells utilized gamma-aminobutyrate as a carbon source and as a N-source by ammonification. The physiological experiments, in particular the inhibition pattern by C2H2, indicated that P. putida expressed an ammonia monooxigenase. DNA-hybridization with an amoA gene probe coding for the smaller subunit of the ammonia monooxigenase of Nitrosomonas europaea allowed us to identify, to clone, and to sequence a region with an open reading frame showing distinct sequence similarities to the amoA gene of autotrophic ammonia oxidizers</description><subject>Aerobiosis</subject><subject>ALEMANIA</subject><subject>ALLEMAGNE</subject><subject>Amino Acid Sequence</subject><subject>Ammonia</subject><subject>Ammonia - metabolism</subject><subject>Ammonification</subject><subject>Anaerobic conditions</subject><subject>BACTERIA</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Carbon sources</subject><subject>Cloning, Molecular</subject><subject>FLORA DEL SUELO</subject><subject>FLORE DU SOL</subject><subject>FOREST SOILS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>GERMANY</subject><subject>Hydroxylamine - metabolism</subject><subject>Metabolism. Enzymes</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nitrates - metabolism</subject><subject>NITRIFICACION</subject><subject>NITRIFICATION</subject><subject>Open Reading Frames</subject><subject>Oxidation-Reduction</subject><subject>Oxidoreductases - genetics</subject><subject>Oxidoreductases - metabolism</subject><subject>Physiology</subject><subject>PSEUDOMONAS PUTIDA</subject><subject>Pseudomonas putida - genetics</subject><subject>Pseudomonas putida - growth & development</subject><subject>Pseudomonas putida - metabolism</subject><subject>Restriction Mapping</subject><subject>Sequence Homology, Amino Acid</subject><subject>SOIL FLORA</subject><subject>SOL DE FORET</subject><subject>SUELO FORESTAL</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkUtr3TAQhUVpSW-TLrssCFq6czt6WJaWIfQRCDTQZG1keRQr2NatZEPSRX571OZyQ7LpamDON2eYOYS8Y_CZATRfMgDX0hgA0ZgXZMOk4BUYw16SDQgpKq1q9pq8yfkagHED7IAcmEbwWjQbcnc-3OYQx3gVnB2pnXs6xRHdOtpEu0fBDTZZt2AKf-wS4kyjp3aa4hwsjTeh3zeXAemABYxLitshODqHJQUfMNHzjGsfy5DNdLsuZeqIvPJ2zPh2Vw_J5bevFyc_qrOf309Pjs8qJ5VcKlQoRM9cx9EZ4Q2onnNAJSR3tYJybecUq602vPPIRMe1Aek7pSRH1CAOyacH322Kv1fMSzuF7HAc7YxxzW0jtFGgmv-CrGFSa6kL-OEZeB3XNJcjWiZ5Ux6vhChU9UC5FHNO6NttCpNNty2D9m987ZP4Cv9-57p2E_Z7epdX0T_udJtLLD7Z2YW8x7jktWbwaONtbO1VKsjlL2b-bVFGiXtJAqwj</recordid><startdate>19981001</startdate><enddate>19981001</enddate><creator>Daum, M. 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(Universitat zu Koln, Koln, Germany.)</au><au>Zimmer, W</au><au>Papen, H</au><au>Kloos, K</au><au>Nawrath, K</au><au>Bothe, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Physiological and molecular biological characterization of ammonia oxidation of the heterotrophic nitrifier Pseudomonas putida</atitle><jtitle>Current microbiology</jtitle><addtitle>Curr Microbiol</addtitle><date>1998-10-01</date><risdate>1998</risdate><volume>37</volume><issue>4</issue><spage>281</spage><epage>288</epage><pages>281-288</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><coden>CUMIDD</coden><abstract>The heterotrophic nitrifier Pseudomonas putida aerobically oxidized ammonia to hydroxylamine, nitrite, and nitrate. Product formation was accompanied by a small but significant release of NO, whereas N2O evolution could not be detected under the assay conditions employed. The isolate reduced nitrate to nitrite and partially further to NO under anaerobic conditions. Aerobically grown cells utilized gamma-aminobutyrate as a carbon source and as a N-source by ammonification. The physiological experiments, in particular the inhibition pattern by C2H2, indicated that P. putida expressed an ammonia monooxigenase. DNA-hybridization with an amoA gene probe coding for the smaller subunit of the ammonia monooxigenase of Nitrosomonas europaea allowed us to identify, to clone, and to sequence a region with an open reading frame showing distinct sequence similarities to the amoA gene of autotrophic ammonia oxidizers</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>9732537</pmid><doi>10.1007/s002849900379</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0343-8651 |
| ispartof | Current microbiology, 1998-10, Vol.37 (4), p.281-288 |
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| subjects | Aerobiosis ALEMANIA ALLEMAGNE Amino Acid Sequence Ammonia Ammonia - metabolism Ammonification Anaerobic conditions BACTERIA Bacteriology Biological and medical sciences Carbon sources Cloning, Molecular FLORA DEL SUELO FLORE DU SOL FOREST SOILS Fundamental and applied biological sciences. Psychology Genes, Bacterial GERMANY Hydroxylamine - metabolism Metabolism. Enzymes Microbiology Molecular Sequence Data Nitrates - metabolism NITRIFICACION NITRIFICATION Open Reading Frames Oxidation-Reduction Oxidoreductases - genetics Oxidoreductases - metabolism Physiology PSEUDOMONAS PUTIDA Pseudomonas putida - genetics Pseudomonas putida - growth & development Pseudomonas putida - metabolism Restriction Mapping Sequence Homology, Amino Acid SOIL FLORA SOL DE FORET SUELO FORESTAL |
| title | Physiological and molecular biological characterization of ammonia oxidation of the heterotrophic nitrifier Pseudomonas putida |
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