Laser line-scanning confocal fluorescence imaging of the photodynamic action of aluminum and zinc phthalocyanines in V79-4 Chinese hamster fibroblasts
Confocal fluorescence microscopy, using a newly constructed laser line-scanning confocal microscope, was applied to an investigation of the early stages of photoinduced destruction of V79-4 Chinese hamster fibroblasts using aluminum and zinc phthalocyanines as photosensitizers. Results obtained in t...
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| Veröffentlicht in: | Photochemistry and photobiology 1998-08, Vol.68 (2), p.199-204 |
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| container_title | Photochemistry and photobiology |
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| creator | Scully, A D Ostler, R B MacRobert, A J Parker, A W de Lara, C O'Neill, P Phillips, D |
| description | Confocal fluorescence microscopy, using a newly constructed laser line-scanning confocal microscope, was applied to an investigation of the early stages of photoinduced destruction of V79-4 Chinese hamster fibroblasts using aluminum and zinc phthalocyanines as photosensitizers. Results obtained in this work show that aluminum and zinc phthalocyanines, once internalized, localize in perinuclear sites that are disrupted upon light exposure resulting in fluorescence redistribution. The combination of laser-line scanning with charge-coupled device detection used in the confocal microscope developed in this work can enable rapid high-resolution sequential imaging, which is ideal for studying photoinduced intracellular fluorescence dynamics. |
| doi_str_mv | 10.1562/0031-8655(1998)068<0199:LLSCFI>2.3.CO;2 |
| format | Article |
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Results obtained in this work show that aluminum and zinc phthalocyanines, once internalized, localize in perinuclear sites that are disrupted upon light exposure resulting in fluorescence redistribution. The combination of laser-line scanning with charge-coupled device detection used in the confocal microscope developed in this work can enable rapid high-resolution sequential imaging, which is ideal for studying photoinduced intracellular fluorescence dynamics.</description><subject>Animals</subject><subject>Cell Line</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>Indoles - pharmacokinetics</subject><subject>Indoles - pharmacology</subject><subject>Lasers</subject><subject>Microscopy, Confocal - instrumentation</subject><subject>Microscopy, Confocal - methods</subject><subject>Microscopy, Fluorescence - instrumentation</subject><subject>Microscopy, Fluorescence - methods</subject><subject>Organometallic Compounds - pharmacokinetics</subject><subject>Organometallic Compounds - pharmacology</subject><subject>Photobiology</subject><subject>Photochemotherapy</subject><subject>Photosensitizing Agents - pharmacology</subject><issn>0031-8655</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kc1u1DAUhb0oakvhESp5hWCRqX8msQ0ICUW0VIo0C6purTs3TmOU2EOcLIYH4XlxNKOurq_O0bF9PkLuONvwshJ3jEle6KosP3Jj9CdW6a8snz43za_6_vGb2MhNvfsiLsj1q_OKvE3pN2N8axS_JJdGCSk4vyb_GkhuooMPrkgIIfjwQjGGLiIMtBuWOLmELqCjfoSXVY0dnXtHD32cY3sMMHqkgLOPYZVgWEYflpFCaOlfHzAb5x6GiEfI4S5RH-izMsWW1v26O9rDmOb8iM7vp7gfIM3pHXnTwZDc-_O8IU_3P57qn0Wze3isvzcFCibmQqBW0gCo0rVat1gprRS41pSia81eothWrOJghGJKITKudbnVCJyjqri8IR9OsYcp_llcmu3o82-HAYKLS7JKaq0NU9n4cDLiFFOaXGcPU-5jOlrO7ArFrlXbtWq7QrEZil2h2BMUK6y09c6KnHR7vnLZj659zTkTkf8BpQeP_g</recordid><startdate>19980801</startdate><enddate>19980801</enddate><creator>Scully, A D</creator><creator>Ostler, R B</creator><creator>MacRobert, A J</creator><creator>Parker, A W</creator><creator>de Lara, C</creator><creator>O'Neill, P</creator><creator>Phillips, D</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980801</creationdate><title>Laser line-scanning confocal fluorescence imaging of the photodynamic action of aluminum and zinc phthalocyanines in V79-4 Chinese hamster fibroblasts</title><author>Scully, A D ; 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| ispartof | Photochemistry and photobiology, 1998-08, Vol.68 (2), p.199-204 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Cell Line Cricetinae Cricetulus Indoles - pharmacokinetics Indoles - pharmacology Lasers Microscopy, Confocal - instrumentation Microscopy, Confocal - methods Microscopy, Fluorescence - instrumentation Microscopy, Fluorescence - methods Organometallic Compounds - pharmacokinetics Organometallic Compounds - pharmacology Photobiology Photochemotherapy Photosensitizing Agents - pharmacology |
| title | Laser line-scanning confocal fluorescence imaging of the photodynamic action of aluminum and zinc phthalocyanines in V79-4 Chinese hamster fibroblasts |
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