Enzymatic formation of cholesteryl ester from cholesterol by gallbladder mucosa

The formation of cholesteryl ester from cholesterol and acyl CoA catalyzed by the enzyme acyl CoA: cholesterol acyltransferase (EC 2.3.1.26) was studied in guinea pig gallbladder mucosa homogenate and the subcellular fractions. The enzymatic activity was enriched in the microsomal fraction. Highest...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Lipids 1981-12, Vol.16 (12), p.930-933
1. Verfasser:	Neiderhiser, Dewey H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acyltransferases - metabolism Animals Bile Acids and Salts - pharmacology Cholesterol - metabolism Cholesterol Esters - biosynthesis Gallbladder - drug effects Gallbladder - enzymology Guinea Pigs In Vitro Techniques Kinetics Microsomes - enzymology Mucous Membrane - metabolism Serum Albumin, Bovine - pharmacology Sterol O-Acyltransferase - metabolism Substrate Specificity
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The formation of cholesteryl ester from cholesterol and acyl CoA catalyzed by the enzyme acyl CoA: cholesterol acyltransferase (EC 2.3.1.26) was studied in guinea pig gallbladder mucosa homogenate and the subcellular fractions. The enzymatic activity was enriched in the microsomal fraction. Highest activity was observed with palmitoyl, stearoyl or oleoyl CoA as substrate. Lowest activity was observed with linoleoyl CoA. These data elucidate one mechanism for the formation of cholesteryl ester from cholesterol by the gallbladder wall.
ISSN:	0024-4201 1558-9307
DOI:	10.1007/BF02535000