Unique morphogenesis in Saccharomyces cerevisiae strain GS1731
During the lag and early exponential phase of growth, 50-60% of budded cells of Saccharomyces cerevisiae strain GS1731 were multiply budded. During subsequent culture growth, the frequency of multiply budded cells decreased until by stationary phase multiply budded cells were rare. Data from renewed...
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| Veröffentlicht in: | FEMS microbiology letters 1998-09, Vol.166 (1), p.95-101 |
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| creator | Singh, V.K McCracken, D.A Nadakavukaren, M.J Jayaswal, R.K |
| description | During the lag and early exponential phase of growth, 50-60% of budded cells of Saccharomyces cerevisiae strain GS1731 were multiply budded. During subsequent culture growth, the frequency of multiply budded cells decreased until by stationary phase multiply budded cells were rare. Data from renewed growth of a culture after hydroxyurea treatment indicated that GS1731 mother cells could assemble up to three pre-bud sites and begin bud growth and development in each. Light and scanning electron microscopy showed two or three very small buds emerging simultaneously on a mother cell and either reaching full size at the same time or enlarging sequentially. Immunofluorescence studies revealed that these multiply budded cells had multiple bundles of cytoplasmic microtubules. DAPI staining of nuclei revealed that some of the unbudded mother cells were multinucleate and completed cytokinesis giving rise to normal daughter cells. |
| doi_str_mv | 10.1111/j.1574-6968.1998.tb13188.x |
| format | Article |
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During subsequent culture growth, the frequency of multiply budded cells decreased until by stationary phase multiply budded cells were rare. Data from renewed growth of a culture after hydroxyurea treatment indicated that GS1731 mother cells could assemble up to three pre-bud sites and begin bud growth and development in each. Light and scanning electron microscopy showed two or three very small buds emerging simultaneously on a mother cell and either reaching full size at the same time or enlarging sequentially. Immunofluorescence studies revealed that these multiply budded cells had multiple bundles of cytoplasmic microtubules. DAPI staining of nuclei revealed that some of the unbudded mother cells were multinucleate and completed cytokinesis giving rise to normal daughter cells.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1998.tb13188.x</identifier><identifier>PMID: 9741087</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Biological and medical sciences ; Budding ; Cell culture ; Cell Cycle ; Cell Nucleus - ultrastructure ; Cytokinesis ; Diploidy ; Electron microscopy ; Fundamental and applied biological sciences. Psychology ; Fungi ; Growth, nutrition, metabolism, transports, enzymes. Molecular biology ; Hydroxyurea ; Immunofluorescence ; Microbiology ; Microscopy, Electron, Scanning ; Microscopy, Fluorescence ; Microtubules ; Microtubules - ultrastructure ; Morphogenesis ; Mycology ; plant biochemistry ; plant breeding ; plant genetics ; plant physiology ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - growth & development ; Saccharomyces cerevisiae - ultrastructure ; Scanning electron microscopy ; Species Specificity ; Stationary phase ; Time Factors ; Yeast</subject><ispartof>FEMS microbiology letters, 1998-09, Vol.166 (1), p.95-101</ispartof><rights>1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. 1998</rights><rights>1998 INIST-CNRS</rights><rights>1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. 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During subsequent culture growth, the frequency of multiply budded cells decreased until by stationary phase multiply budded cells were rare. Data from renewed growth of a culture after hydroxyurea treatment indicated that GS1731 mother cells could assemble up to three pre-bud sites and begin bud growth and development in each. Light and scanning electron microscopy showed two or three very small buds emerging simultaneously on a mother cell and either reaching full size at the same time or enlarging sequentially. Immunofluorescence studies revealed that these multiply budded cells had multiple bundles of cytoplasmic microtubules. DAPI staining of nuclei revealed that some of the unbudded mother cells were multinucleate and completed cytokinesis giving rise to normal daughter cells.</description><subject>Biological and medical sciences</subject><subject>Budding</subject><subject>Cell culture</subject><subject>Cell Cycle</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Cytokinesis</subject><subject>Diploidy</subject><subject>Electron microscopy</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungi</subject><subject>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</subject><subject>Hydroxyurea</subject><subject>Immunofluorescence</subject><subject>Microbiology</subject><subject>Microscopy, Electron, Scanning</subject><subject>Microscopy, Fluorescence</subject><subject>Microtubules</subject><subject>Microtubules - ultrastructure</subject><subject>Morphogenesis</subject><subject>Mycology</subject><subject>plant biochemistry</subject><subject>plant breeding</subject><subject>plant genetics</subject><subject>plant physiology</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - growth & development</subject><subject>Saccharomyces cerevisiae - ultrastructure</subject><subject>Scanning electron microscopy</subject><subject>Species Specificity</subject><subject>Stationary phase</subject><subject>Time Factors</subject><subject>Yeast</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqVkU9v1DAQxS0EKsvCR0BEgLgljD2O7SBRCVW0IC3isOzZ8rpO61X-bO0N7X57HCXaAwIhfJnD-73xmxlCXlMoaHrvdwUtJc9FJVRBq0oVhy1FqlTx8IgsTtJjsgCUKqdQyafkWYw7AOAMxBk5qySnoOSCnG86fze4rO3D_ra_cZ2LPma-y9bG2lsT-vZoXcysC-6nj964LB6CSfrVmkqkz8mT2jTRvZjrkmwuP_-4-JKvvl99vfi0yi0vscxLZg0gBwGsQnCSKgGmrA06YVGANU6grSUKRh0w4ALAKVRbpajihl_jkryb-u5Dn-LGg259tK5pTOf6IWqJSvFS8H-CVJQ8dR7BN7-Bu34IXRpCM0TKBatSXZIPE2VDH2Nwtd4H35pw1BT0eAu90-PC9bhwPd5Cz7fQD8n8cv5i2Lbu-mSdl5_0t7NuojVNHUxnfTxhjFPBVJWwjxN27xt3_I8A-vLbqiqTv5z8_bD_izv_c_xXk682vTY3IUXbrBlQhJSKo0T8BZyOu64</recordid><startdate>199809</startdate><enddate>199809</enddate><creator>Singh, V.K</creator><creator>McCracken, D.A</creator><creator>Nadakavukaren, M.J</creator><creator>Jayaswal, R.K</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199809</creationdate><title>Unique morphogenesis in Saccharomyces cerevisiae strain GS1731</title><author>Singh, V.K ; McCracken, D.A ; Nadakavukaren, M.J ; Jayaswal, R.K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4535-52ca0340602930e71860a5fa3e6c360cae63cf73621e0204600e838b88184a4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Biological and medical sciences</topic><topic>Budding</topic><topic>Cell culture</topic><topic>Cell Cycle</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Cytokinesis</topic><topic>Diploidy</topic><topic>Electron microscopy</topic><topic>Fundamental and applied biological sciences. 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During subsequent culture growth, the frequency of multiply budded cells decreased until by stationary phase multiply budded cells were rare. Data from renewed growth of a culture after hydroxyurea treatment indicated that GS1731 mother cells could assemble up to three pre-bud sites and begin bud growth and development in each. Light and scanning electron microscopy showed two or three very small buds emerging simultaneously on a mother cell and either reaching full size at the same time or enlarging sequentially. Immunofluorescence studies revealed that these multiply budded cells had multiple bundles of cytoplasmic microtubules. DAPI staining of nuclei revealed that some of the unbudded mother cells were multinucleate and completed cytokinesis giving rise to normal daughter cells.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>9741087</pmid><doi>10.1111/j.1574-6968.1998.tb13188.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1998-09, Vol.166 (1), p.95-101 |
| issn | 0378-1097 1574-6968 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Biological and medical sciences Budding Cell culture Cell Cycle Cell Nucleus - ultrastructure Cytokinesis Diploidy Electron microscopy Fundamental and applied biological sciences. Psychology Fungi Growth, nutrition, metabolism, transports, enzymes. Molecular biology Hydroxyurea Immunofluorescence Microbiology Microscopy, Electron, Scanning Microscopy, Fluorescence Microtubules Microtubules - ultrastructure Morphogenesis Mycology plant biochemistry plant breeding plant genetics plant physiology Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae - ultrastructure Scanning electron microscopy Species Specificity Stationary phase Time Factors Yeast |
| title | Unique morphogenesis in Saccharomyces cerevisiae strain GS1731 |
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