Purification and Some Properties of 2-Hydroxychromene-2-Carboxylate Isomerase from Naphthalenesulfonate-Assimilating Pseudomonas sp. TA-2

A 2-hydroxychromene-2-carboxylate isomerase was purified from a cell-free extract of naphthalenesulfonate-assimilating Pseudomonas sp. TA-2 to an electrophoretically homogeneous state by successive column chromatography on DEAE-cellulose, DEAE-Toyopearl 650M, Sephadex G-75, Hydroxyapatite, and Mono...

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Veröffentlicht in:	Journal of biochemistry (Tokyo) 1998-09, Vol.124 (3), p.591-597
Hauptverfasser:	Ohmoto, Takashi, Kinoshita, Toshihiko, Moriyoshi, Kunihiko, Sakai, Kiyofumi, Hamada, Nobutake, Ohe, Tatsuhiko
Format:	Artikel
Sprache:	eng
Schlagworte:	2-hydroxychromene-2 carboxylate Amino Acid Sequence Cell-Free System Chromatography, High Pressure Liquid Chromatography, Ion Exchange cis-trans isomerase Electrophoresis, Polyacrylamide Gel Enzyme Activation Hydrogen-Ion Concentration Intramolecular Oxidoreductases - chemistry Intramolecular Oxidoreductases - isolation & purification Intramolecular Oxidoreductases - metabolism Molecular Sequence Data Molecular Weight naphthalene naphthalenesulfonate Naphthalenesulfonates - metabolism Pseudomonas Pseudomonas - enzymology Sequence Homology, Amino Acid Temperature
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