Speciation of selenium in the mammalian organism
As almost all of the selenium present in the mammalian organism is protein-bound, speciation is mostly concerned with the determination of the different selenium-containing proteins. Information on their distribution and their concentrations in the different tissues of the rat was obtained by means...
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| Veröffentlicht in: | Analyst (London) 1998-05, Vol.123 (5), p.871-873 |
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| container_title | Analyst (London) |
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| creator | BEHNE, D HAMMEL, C PFEIFER, H RÖTHLEIN, D GESSNER, H KYRIAKOPOULOS, A |
| description | As almost all of the selenium present in the mammalian organism is protein-bound, speciation is mostly concerned with the determination of the different selenium-containing proteins. Information on their distribution and their concentrations in the different tissues of the rat was obtained by means of tracer procedures which, after application of 75Se-selenite with a very high specific activity to selenium-depleted animals and electrophoretic separation of the labelled proteins, allow the determination of these compounds in the pmol to fmos range. A method was developed for the determination of selenocysteine and selenomethionine in the selenium-containing proteins. The identification of specific selenoproteins was achieved by analysis of their selenoamino acid residues and by studies on their characteristics and possible biological functions. This is being followed by the development of methods for the quantitative analysis of the selenoproteins in questions in the tissues of animals and man. In this paper the strategies and procedures used in the identification, characterization and determination of the selenium species present in the mammalian organism will be discussed. |
| doi_str_mv | 10.1039/a708699g |
| format | Article |
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Information on their distribution and their concentrations in the different tissues of the rat was obtained by means of tracer procedures which, after application of 75Se-selenite with a very high specific activity to selenium-depleted animals and electrophoretic separation of the labelled proteins, allow the determination of these compounds in the pmol to fmos range. A method was developed for the determination of selenocysteine and selenomethionine in the selenium-containing proteins. The identification of specific selenoproteins was achieved by analysis of their selenoamino acid residues and by studies on their characteristics and possible biological functions. This is being followed by the development of methods for the quantitative analysis of the selenoproteins in questions in the tissues of animals and man. In this paper the strategies and procedures used in the identification, characterization and determination of the selenium species present in the mammalian organism will be discussed.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/a708699g</identifier><identifier>PMID: 9709480</identifier><identifier>CODEN: ANALAO</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Fundamental and applied biological sciences. 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Information on their distribution and their concentrations in the different tissues of the rat was obtained by means of tracer procedures which, after application of 75Se-selenite with a very high specific activity to selenium-depleted animals and electrophoretic separation of the labelled proteins, allow the determination of these compounds in the pmol to fmos range. A method was developed for the determination of selenocysteine and selenomethionine in the selenium-containing proteins. The identification of specific selenoproteins was achieved by analysis of their selenoamino acid residues and by studies on their characteristics and possible biological functions. This is being followed by the development of methods for the quantitative analysis of the selenoproteins in questions in the tissues of animals and man. 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Psychology</subject><subject>Inorganic compounds</subject><subject>Other biological molecules</subject><subject>Proteins - chemistry</subject><subject>Rats</subject><subject>Selenium Radioisotopes</subject><subject>Selenocysteine - analysis</subject><subject>Selenomethionine - analysis</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1KxDAYRYMo4zgKvoDQhYib6pek-VvK4B8MuFDX5WsmGSNNOzbtwrefytRZXS73cBeHkEsKdxS4uUcFWhqzOSJzymWRC8H0MZkDAM-ZFMUpOUvpe6wUBMzIzCgwhYY5gfetswH70DZZ67PkateEIWahyfovl0WMEeuA49htsAkpnpMTj3VyF1MuyOfT48fyJV-9Pb8uH1a55Uz1ubHCeCiUoZwLoZUBid4WyklGNa_M2D0WSkukKKRGsWaGSkFdZSsBzPMFudn_brv2Z3CpL2NI1tU1Nq4dUqm4loWRdARv96Dt2pQ658ttFyJ2vyWF8k9O-S9nRK-mz6GKbn0AJxvjfj3tmCzWvsPGhnTAGAcuheQ7OcBpOg</recordid><startdate>19980501</startdate><enddate>19980501</enddate><creator>BEHNE, D</creator><creator>HAMMEL, C</creator><creator>PFEIFER, H</creator><creator>RÖTHLEIN, D</creator><creator>GESSNER, H</creator><creator>KYRIAKOPOULOS, A</creator><general>Royal Society of Chemistry</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980501</creationdate><title>Speciation of selenium in the mammalian organism</title><author>BEHNE, D ; HAMMEL, C ; PFEIFER, H ; RÖTHLEIN, D ; GESSNER, H ; KYRIAKOPOULOS, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c327t-9c59f04791335587906afc47e62183b9906fa4786a1a568a5d291651ebcb502f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Inorganic compounds</topic><topic>Other biological molecules</topic><topic>Proteins - chemistry</topic><topic>Rats</topic><topic>Selenium Radioisotopes</topic><topic>Selenocysteine - analysis</topic><topic>Selenomethionine - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BEHNE, D</creatorcontrib><creatorcontrib>HAMMEL, C</creatorcontrib><creatorcontrib>PFEIFER, H</creatorcontrib><creatorcontrib>RÖTHLEIN, D</creatorcontrib><creatorcontrib>GESSNER, H</creatorcontrib><creatorcontrib>KYRIAKOPOULOS, A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BEHNE, D</au><au>HAMMEL, C</au><au>PFEIFER, H</au><au>RÖTHLEIN, D</au><au>GESSNER, H</au><au>KYRIAKOPOULOS, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Speciation of selenium in the mammalian organism</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>1998-05-01</date><risdate>1998</risdate><volume>123</volume><issue>5</issue><spage>871</spage><epage>873</epage><pages>871-873</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><coden>ANALAO</coden><abstract>As almost all of the selenium present in the mammalian organism is protein-bound, speciation is mostly concerned with the determination of the different selenium-containing proteins. Information on their distribution and their concentrations in the different tissues of the rat was obtained by means of tracer procedures which, after application of 75Se-selenite with a very high specific activity to selenium-depleted animals and electrophoretic separation of the labelled proteins, allow the determination of these compounds in the pmol to fmos range. A method was developed for the determination of selenocysteine and selenomethionine in the selenium-containing proteins. The identification of specific selenoproteins was achieved by analysis of their selenoamino acid residues and by studies on their characteristics and possible biological functions. This is being followed by the development of methods for the quantitative analysis of the selenoproteins in questions in the tissues of animals and man. In this paper the strategies and procedures used in the identification, characterization and determination of the selenium species present in the mammalian organism will be discussed.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><pmid>9709480</pmid><doi>10.1039/a708699g</doi><tpages>3</tpages></addata></record> |
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| ispartof | Analyst (London), 1998-05, Vol.123 (5), p.871-873 |
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| language | eng |
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| source | Royal Society of Chemistry Journals Archive (1841-2007); MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Fundamental and applied biological sciences. Psychology Inorganic compounds Other biological molecules Proteins - chemistry Rats Selenium Radioisotopes Selenocysteine - analysis Selenomethionine - analysis |
| title | Speciation of selenium in the mammalian organism |
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