Predictive model for capillary electrophoretic peptide mobility in 2,2,2-trifluoroethanol-water solution
Using capillary electrophoresis (CE) on a set of 21 peptides with a molecular mass ranging from about 350 to 1850 Da, the Stokes radii at different protonation stages and the acidic dissociation constants in water and in a 2,2,2‐trifluoroethanol (TFE) water mixture (30% v/v) were determined. These r...
Gespeichert in:
| Veröffentlicht in: | Electrophoresis 1998-07, Vol.19 (10), p.1728-1732 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1732 |
|---|---|
| container_issue | 10 |
| container_start_page | 1728 |
| container_title | Electrophoresis |
| container_volume | 19 |
| creator | Castagnola, Massimo Rossetti, Diana Valeria Corda, Marcella Pellegrini, Mariagiuseppina Misiti, Francesco Olianas, Alessandra Giardina, Bruno Messana, Irene |
| description | Using capillary electrophoresis (CE) on a set of 21 peptides with a molecular mass ranging from about 350 to 1850 Da, the Stokes radii at different protonation stages and the acidic dissociation constants in water and in a 2,2,2‐trifluoroethanol (TFE) water mixture (30% v/v) were determined. These results permitted us to establish separately the reliability of semiempirical models utilized for the prediction of peptide size and charge at different acidic pHapp (pHapp range: 2.00–4.25). The data obtained on size and charge were utilized in order to provide suitable mobility predictions on the basis of the charge‐to‐size ratio. The best predictive conditions for size and charge were found at the most acidic range of pHapp studied (2.00–2.25), either in water or a TFE‐water mixture, and reliable predictive equations for peptide mobility were established at this pHapp. |
| doi_str_mv | 10.1002/elps.1150191033 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73857091</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73857091</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3823-10416c91601e33e0ef0e31f2d48323126657393b41f14a3a559655875770bb063</originalsourceid><addsrcrecordid>eNqFkM1vEzEQxS0EKmnhzAlpT5zY1uNZ22txQlUbEBFUosDR2o9ZxeDEW9tLm_-ejRIVcUJzmMP7vSe9x9gr4OfAubggP6ZzAMnBAEd8whYghSiFqvEpW3DQWPIa5XN2mtJPznllquqEnRgNRkqxYOubSL3rsvtNxSb05IshxKJrRud9E3cFeepyDOM6RMquK0Yas-v3bOu8y7vCbQvxdr4yRzf4KcRAed1sgy_vm0yxSMFP2YXtC_ZsaHyil8d_xr5dX91efihXX5YfL9-vyg5rgSXwClRnQHEgROI0cEIYRF_VKBCEUlKjwbaCAaoGGymNkrLWUmvetlzhGXtzyB1juJsoZbtxqaO5zZbClKzGWmpuYAYvDmAXQ0qRBjtGt5k7W-B2v63db2v_bjs7Xh-jp3ZD_SN_HHPW3x30e-dp9784e7W6-fpPenlwu5Tp4dHdxF9WadTS_vi8tMvv2ohP17dW4R9LyJVa</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73857091</pqid></control><display><type>article</type><title>Predictive model for capillary electrophoretic peptide mobility in 2,2,2-trifluoroethanol-water solution</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Castagnola, Massimo ; Rossetti, Diana Valeria ; Corda, Marcella ; Pellegrini, Mariagiuseppina ; Misiti, Francesco ; Olianas, Alessandra ; Giardina, Bruno ; Messana, Irene</creator><creatorcontrib>Castagnola, Massimo ; Rossetti, Diana Valeria ; Corda, Marcella ; Pellegrini, Mariagiuseppina ; Misiti, Francesco ; Olianas, Alessandra ; Giardina, Bruno ; Messana, Irene</creatorcontrib><description>Using capillary electrophoresis (CE) on a set of 21 peptides with a molecular mass ranging from about 350 to 1850 Da, the Stokes radii at different protonation stages and the acidic dissociation constants in water and in a 2,2,2‐trifluoroethanol (TFE) water mixture (30% v/v) were determined. These results permitted us to establish separately the reliability of semiempirical models utilized for the prediction of peptide size and charge at different acidic pHapp (pHapp range: 2.00–4.25). The data obtained on size and charge were utilized in order to provide suitable mobility predictions on the basis of the charge‐to‐size ratio. The best predictive conditions for size and charge were found at the most acidic range of pHapp studied (2.00–2.25), either in water or a TFE‐water mixture, and reliable predictive equations for peptide mobility were established at this pHapp.</description><identifier>ISSN: 0173-0835</identifier><identifier>EISSN: 1522-2683</identifier><identifier>DOI: 10.1002/elps.1150191033</identifier><identifier>PMID: 9719552</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>2,2,2‐Trifluoroethanol ; 2-Trifluoroethanol ; Capillary electrophoresis ; Electrophoresis, Capillary - methods ; Mathematical Computing ; Models, Molecular ; Peptide ; Peptides - analysis ; Predictive models ; Solutions ; Stokes radius ; Trifluoroethanol ; Water</subject><ispartof>Electrophoresis, 1998-07, Vol.19 (10), p.1728-1732</ispartof><rights>Copyright © 1998 Wiley‐VCH Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3823-10416c91601e33e0ef0e31f2d48323126657393b41f14a3a559655875770bb063</citedby><cites>FETCH-LOGICAL-c3823-10416c91601e33e0ef0e31f2d48323126657393b41f14a3a559655875770bb063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Felps.1150191033$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Felps.1150191033$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9719552$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Castagnola, Massimo</creatorcontrib><creatorcontrib>Rossetti, Diana Valeria</creatorcontrib><creatorcontrib>Corda, Marcella</creatorcontrib><creatorcontrib>Pellegrini, Mariagiuseppina</creatorcontrib><creatorcontrib>Misiti, Francesco</creatorcontrib><creatorcontrib>Olianas, Alessandra</creatorcontrib><creatorcontrib>Giardina, Bruno</creatorcontrib><creatorcontrib>Messana, Irene</creatorcontrib><title>Predictive model for capillary electrophoretic peptide mobility in 2,2,2-trifluoroethanol-water solution</title><title>Electrophoresis</title><addtitle>ELECTROPHORESIS</addtitle><description>Using capillary electrophoresis (CE) on a set of 21 peptides with a molecular mass ranging from about 350 to 1850 Da, the Stokes radii at different protonation stages and the acidic dissociation constants in water and in a 2,2,2‐trifluoroethanol (TFE) water mixture (30% v/v) were determined. These results permitted us to establish separately the reliability of semiempirical models utilized for the prediction of peptide size and charge at different acidic pHapp (pHapp range: 2.00–4.25). The data obtained on size and charge were utilized in order to provide suitable mobility predictions on the basis of the charge‐to‐size ratio. The best predictive conditions for size and charge were found at the most acidic range of pHapp studied (2.00–2.25), either in water or a TFE‐water mixture, and reliable predictive equations for peptide mobility were established at this pHapp.</description><subject>2,2,2‐Trifluoroethanol</subject><subject>2-Trifluoroethanol</subject><subject>Capillary electrophoresis</subject><subject>Electrophoresis, Capillary - methods</subject><subject>Mathematical Computing</subject><subject>Models, Molecular</subject><subject>Peptide</subject><subject>Peptides - analysis</subject><subject>Predictive models</subject><subject>Solutions</subject><subject>Stokes radius</subject><subject>Trifluoroethanol</subject><subject>Water</subject><issn>0173-0835</issn><issn>1522-2683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1vEzEQxS0EKmnhzAlpT5zY1uNZ22txQlUbEBFUosDR2o9ZxeDEW9tLm_-ejRIVcUJzmMP7vSe9x9gr4OfAubggP6ZzAMnBAEd8whYghSiFqvEpW3DQWPIa5XN2mtJPznllquqEnRgNRkqxYOubSL3rsvtNxSb05IshxKJrRud9E3cFeepyDOM6RMquK0Yas-v3bOu8y7vCbQvxdr4yRzf4KcRAed1sgy_vm0yxSMFP2YXtC_ZsaHyil8d_xr5dX91efihXX5YfL9-vyg5rgSXwClRnQHEgROI0cEIYRF_VKBCEUlKjwbaCAaoGGymNkrLWUmvetlzhGXtzyB1juJsoZbtxqaO5zZbClKzGWmpuYAYvDmAXQ0qRBjtGt5k7W-B2v63db2v_bjs7Xh-jp3ZD_SN_HHPW3x30e-dp9784e7W6-fpPenlwu5Tp4dHdxF9WadTS_vi8tMvv2ohP17dW4R9LyJVa</recordid><startdate>199807</startdate><enddate>199807</enddate><creator>Castagnola, Massimo</creator><creator>Rossetti, Diana Valeria</creator><creator>Corda, Marcella</creator><creator>Pellegrini, Mariagiuseppina</creator><creator>Misiti, Francesco</creator><creator>Olianas, Alessandra</creator><creator>Giardina, Bruno</creator><creator>Messana, Irene</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199807</creationdate><title>Predictive model for capillary electrophoretic peptide mobility in 2,2,2-trifluoroethanol-water solution</title><author>Castagnola, Massimo ; Rossetti, Diana Valeria ; Corda, Marcella ; Pellegrini, Mariagiuseppina ; Misiti, Francesco ; Olianas, Alessandra ; Giardina, Bruno ; Messana, Irene</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3823-10416c91601e33e0ef0e31f2d48323126657393b41f14a3a559655875770bb063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>2,2,2‐Trifluoroethanol</topic><topic>2-Trifluoroethanol</topic><topic>Capillary electrophoresis</topic><topic>Electrophoresis, Capillary - methods</topic><topic>Mathematical Computing</topic><topic>Models, Molecular</topic><topic>Peptide</topic><topic>Peptides - analysis</topic><topic>Predictive models</topic><topic>Solutions</topic><topic>Stokes radius</topic><topic>Trifluoroethanol</topic><topic>Water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Castagnola, Massimo</creatorcontrib><creatorcontrib>Rossetti, Diana Valeria</creatorcontrib><creatorcontrib>Corda, Marcella</creatorcontrib><creatorcontrib>Pellegrini, Mariagiuseppina</creatorcontrib><creatorcontrib>Misiti, Francesco</creatorcontrib><creatorcontrib>Olianas, Alessandra</creatorcontrib><creatorcontrib>Giardina, Bruno</creatorcontrib><creatorcontrib>Messana, Irene</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Electrophoresis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Castagnola, Massimo</au><au>Rossetti, Diana Valeria</au><au>Corda, Marcella</au><au>Pellegrini, Mariagiuseppina</au><au>Misiti, Francesco</au><au>Olianas, Alessandra</au><au>Giardina, Bruno</au><au>Messana, Irene</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Predictive model for capillary electrophoretic peptide mobility in 2,2,2-trifluoroethanol-water solution</atitle><jtitle>Electrophoresis</jtitle><addtitle>ELECTROPHORESIS</addtitle><date>1998-07</date><risdate>1998</risdate><volume>19</volume><issue>10</issue><spage>1728</spage><epage>1732</epage><pages>1728-1732</pages><issn>0173-0835</issn><eissn>1522-2683</eissn><abstract>Using capillary electrophoresis (CE) on a set of 21 peptides with a molecular mass ranging from about 350 to 1850 Da, the Stokes radii at different protonation stages and the acidic dissociation constants in water and in a 2,2,2‐trifluoroethanol (TFE) water mixture (30% v/v) were determined. These results permitted us to establish separately the reliability of semiempirical models utilized for the prediction of peptide size and charge at different acidic pHapp (pHapp range: 2.00–4.25). The data obtained on size and charge were utilized in order to provide suitable mobility predictions on the basis of the charge‐to‐size ratio. The best predictive conditions for size and charge were found at the most acidic range of pHapp studied (2.00–2.25), either in water or a TFE‐water mixture, and reliable predictive equations for peptide mobility were established at this pHapp.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>9719552</pmid><doi>10.1002/elps.1150191033</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0173-0835 |
| ispartof | Electrophoresis, 1998-07, Vol.19 (10), p.1728-1732 |
| issn | 0173-0835 1522-2683 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73857091 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | 2,2,2‐Trifluoroethanol 2-Trifluoroethanol Capillary electrophoresis Electrophoresis, Capillary - methods Mathematical Computing Models, Molecular Peptide Peptides - analysis Predictive models Solutions Stokes radius Trifluoroethanol Water |
| title | Predictive model for capillary electrophoretic peptide mobility in 2,2,2-trifluoroethanol-water solution |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T22%3A52%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Predictive%20model%20for%20capillary%20electrophoretic%20peptide%20mobility%20in%202,2,2-trifluoroethanol-water%20solution&rft.jtitle=Electrophoresis&rft.au=Castagnola,%20Massimo&rft.date=1998-07&rft.volume=19&rft.issue=10&rft.spage=1728&rft.epage=1732&rft.pages=1728-1732&rft.issn=0173-0835&rft.eissn=1522-2683&rft_id=info:doi/10.1002/elps.1150191033&rft_dat=%3Cproquest_cross%3E73857091%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=73857091&rft_id=info:pmid/9719552&rfr_iscdi=true |