Antibacterial activity of bovine lactoferrin and its peptides against enterohaemorrhagic Escherichia coli O157:H7
The antimicrobial activities of bovine lactoferrin (bLF), its pepsin hydrolysate (bLFH) and the active peptide lactoferricin® B (LFcinB) against four clinical isolates of enterohaemorrhagic Escherichia coli O157:H7 were studied. The MICs against these isolates were 3 mg ml−1 for bLF, 0·1–0·2 mg ml−1...
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| Veröffentlicht in: | Letters in applied microbiology 1998-06, Vol.26 (6), p.407-411 |
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| container_title | Letters in applied microbiology |
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| creator | SHIN, K YAMAUCHI, K TERAGUCHI, S HAYASAWA, H TOMITA, M OTSUKA, Y YAMAZAKI, S |
| description | The antimicrobial activities of bovine lactoferrin (bLF), its pepsin hydrolysate (bLFH) and the active peptide lactoferricin® B (LFcinB) against four clinical isolates of enterohaemorrhagic Escherichia coli O157:H7 were studied. The MICs against these isolates were 3 mg ml−1 for bLF, 0·1–0·2 mg ml−1 for bLFH and 8–10 μg ml−1 for LFcinB in 1% Bactopeptone broth. LFcinB killed these bacteria within 3 h at concentrations above 10 μg ml−1. Transmission electron microscopy findings suggested that LFcinB acts on the bacterial surface and affects cytoplasmic contents. LFcinB was shown to influence the levels of verotoxins in the culture supernatant fluid of an E. coli 0157:H7 strain. These results demonstrate that E. coli O157:H7 strains are susceptible to the antimicrobial effects of bLF and its peptides. |
| doi_str_mv | 10.1046/j.1472-765X.1998.00358.x |
| format | Article |
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The MICs against these isolates were 3 mg ml−1 for bLF, 0·1–0·2 mg ml−1 for bLFH and 8–10 μg ml−1 for LFcinB in 1% Bactopeptone broth. LFcinB killed these bacteria within 3 h at concentrations above 10 μg ml−1. Transmission electron microscopy findings suggested that LFcinB acts on the bacterial surface and affects cytoplasmic contents. LFcinB was shown to influence the levels of verotoxins in the culture supernatant fluid of an E. coli 0157:H7 strain. These results demonstrate that E. coli O157:H7 strains are susceptible to the antimicrobial effects of bLF and its peptides.</description><identifier>ISSN: 0266-8254</identifier><identifier>EISSN: 1472-765X</identifier><identifier>DOI: 10.1046/j.1472-765X.1998.00358.x</identifier><identifier>PMID: 9717310</identifier><identifier>CODEN: LAMIE7</identifier><language>eng</language><publisher>Edinburgh, UK: Blackwell Science Ltd</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; Antibacterial agents ; Antibiotics. 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The MICs against these isolates were 3 mg ml−1 for bLF, 0·1–0·2 mg ml−1 for bLFH and 8–10 μg ml−1 for LFcinB in 1% Bactopeptone broth. LFcinB killed these bacteria within 3 h at concentrations above 10 μg ml−1. Transmission electron microscopy findings suggested that LFcinB acts on the bacterial surface and affects cytoplasmic contents. LFcinB was shown to influence the levels of verotoxins in the culture supernatant fluid of an E. coli 0157:H7 strain. These results demonstrate that E. coli O157:H7 strains are susceptible to the antimicrobial effects of bLF and its peptides.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibacterial agents</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Bacterial Toxins - analysis</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - ultrastructure</subject><subject>Cytoplasm - drug effects</subject><subject>Cytoplasm - ultrastructure</subject><subject>Escherichia coli</subject><subject>Escherichia coli O157 - chemistry</subject><subject>Escherichia coli O157 - drug effects</subject><subject>Humans</subject><subject>Lactoferrin - analogs & derivatives</subject><subject>Lactoferrin - pharmacology</subject><subject>Medical sciences</subject><subject>Microbial Sensitivity Tests</subject><subject>Microscopy, Electron</subject><subject>Peptide Fragments - pharmacology</subject><subject>Pharmacology. 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The MICs against these isolates were 3 mg ml−1 for bLF, 0·1–0·2 mg ml−1 for bLFH and 8–10 μg ml−1 for LFcinB in 1% Bactopeptone broth. LFcinB killed these bacteria within 3 h at concentrations above 10 μg ml−1. Transmission electron microscopy findings suggested that LFcinB acts on the bacterial surface and affects cytoplasmic contents. LFcinB was shown to influence the levels of verotoxins in the culture supernatant fluid of an E. coli 0157:H7 strain. These results demonstrate that E. coli O157:H7 strains are susceptible to the antimicrobial effects of bLF and its peptides.</abstract><cop>Edinburgh, UK</cop><pub>Blackwell Science Ltd</pub><pmid>9717310</pmid><doi>10.1046/j.1472-765X.1998.00358.x</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Letters in applied microbiology, 1998-06, Vol.26 (6), p.407-411 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Anti-Bacterial Agents - pharmacology Antibacterial agents Antibiotics. Antiinfectious agents. Antiparasitic agents Bacterial Toxins - analysis Biological and medical sciences Cattle Cell Membrane - drug effects Cell Membrane - ultrastructure Cytoplasm - drug effects Cytoplasm - ultrastructure Escherichia coli Escherichia coli O157 - chemistry Escherichia coli O157 - drug effects Humans Lactoferrin - analogs & derivatives Lactoferrin - pharmacology Medical sciences Microbial Sensitivity Tests Microscopy, Electron Peptide Fragments - pharmacology Pharmacology. Drug treatments |
| title | Antibacterial activity of bovine lactoferrin and its peptides against enterohaemorrhagic Escherichia coli O157:H7 |
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