Rapid electrophoretic separation of pre-beta-migrating high density lipoproteins using automated PhastSystem: application to analysis of lecithin:cholesterol acyltransferase-deficient plasma

To identify pre-beta-high density lipoproteins, a rapid two-dimensional separation by electrophoresis (1 hour 30 minutes) was performed on an automated Phast System. This procedure used commercially available polyacrylamide gradient gels (4-15%) and allows sensitive and reproducible results. Pre-bet...

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Veröffentlicht in:	Clinical chemistry and laboratory medicine 1998-06, Vol.36 (6), p.385-387
Hauptverfasser:	Saïdi, Y, Sich, D, Federspiel, M C, Auer, C, Egloff, M, Castro, G R, Beucler, I
Format:	Artikel
Sprache:	eng
Schlagworte:	Automation Electrophoresis, Agar Gel - methods Electrophoresis, Gel, Two-Dimensional Humans Lecithin Cholesterol Acyltransferase Deficiency - blood Lecithin Cholesterol Acyltransferase Deficiency - diagnosis Lipoproteins, HDL - isolation & purification Phosphatidylcholine-Sterol O-Acyltransferase - blood Protein Precursors - isolation & purification
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