Nuclear sphingomyelin protects RNA from RNase action

Chromatin phospholipidic fraction, as previously demonstrated, shows the same localization as RNA inside the nuclei. DNase and RNase treatment of nuclei removed almost totally the DNA, 63% of RNA and caused a 50% loss of phospholipids. The aim of the present investigation is to study the fraction of...

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Veröffentlicht in:	FEBS letters 1998-07, Vol.431 (3), p.443-447
Hauptverfasser:	Micheli, Marta, Albi, Elisabetta, Leray, Claude, Magni, Mariapia Viola
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Nucleus - enzymology Cell Nucleus - metabolism Cells, Cultured DAG, diacylglycerol DNT, digested nuclei after Triton X-100 treatment Double-stranded RNA EDTA, ethylendiaminetetra-acetic acid Female Liver - cytology Liver - enzymology Liver - metabolism Male N-SMase, neutral sphingomyelinase NT, nuclei after Triton X-100 treatment PC, phosphatidylcholine PC-PLC, phosphatidylcholine-dependent phospholipase C PE, phosphatidylethanolamine Phosphatidylcholine Phosphatidylcholine-dependent phospholipase C Phosphatidylcholines - metabolism PI, phosphatidylinositol PLs, phospholipids PS, phosphatidylserine Rats Rats, Sprague-Dawley Ribonucleases - metabolism RNA - metabolism SM synthase, sphingomyelin synthase SM, sphingomyelin Sphingomyelin Sphingomyelin Phosphodiesterase - metabolism Sphingomyelin synthase Sphingomyelinase Sphingomyelins - metabolism TLC, thin layer chromatography Tris, hydroxymethylaminomethane Type C Phospholipases - metabolism
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