Bovine Lactoferrin Stimulates the Phagocytic Activity of Human Neutrophils: Identification of Its Active Domain
Bovine LF (bLF) at concentrations in the range of 50–250 μg/ml enhanced the phagocytic activity of human neutrophils as determined by measuring the incorporation of FITC-labeled latex beads by flow cytometry. The stimulatory effect of bLF was not abrogated by hydrolysis with pepsin. Bovine lactoferr...
Gespeichert in:
| Veröffentlicht in: | Cellular immunology 1998-07, Vol.187 (1), p.34-37 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 37 |
|---|---|
| container_issue | 1 |
| container_start_page | 34 |
| container_title | Cellular immunology |
| container_volume | 187 |
| creator | Miyauchi, Hirofumi Hashimoto, Shin-ichi Nakajima, Mitsunari Shinoda, Ichizo Fukuwatari, Yasuo Hayasawa, Hirotoshi |
| description | Bovine LF (bLF) at concentrations in the range of 50–250 μg/ml enhanced the phagocytic activity of human neutrophils as determined by measuring the incorporation of FITC-labeled latex beads by flow cytometry. The stimulatory effect of bLF was not abrogated by hydrolysis with pepsin. Bovine lactoferricin (bLFcin), which is a bactericidal fragment purified from a pepsin hydrolysate of bLF (bLFH), also enhanced the phagocytic activity, whereas, in contrast, the fraction of bLFH depleted of bLFcin showed no stimulatory effect. The phagocytosis-enhancing activity of bLF still remained after washing the neutrophils, following exposure to bLF. Also, bLF pretreatment of the latex beads stimulated their uptake. These results demonstrate that bLF is effective in promoting the phagocytic activity of human neutrophils. This activity appears to be due to its bLFcin domain and may involve dual mechanisms of direct binding to neutrophils and opsonin-like activity. |
| doi_str_mv | 10.1006/cimm.1997.1246 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73845858</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0008874997912466</els_id><sourcerecordid>73845858</sourcerecordid><originalsourceid>FETCH-LOGICAL-c436t-7a1de41eab65667ae2e705372c7667ed355ccf88b0c4b95e39c3d3f570a67ef03</originalsourceid><addsrcrecordid>eNqFkb1v2zAUxImgReKkXbMF4NRNLilK_OiWpE1jwGgLNJ0JmnqKXyCJDkkZ8H8fCTa6FZ0eDve7G94Rcs3ZkjMmP3vs-yU3Ri15WckzsuDMsKLkUrwjC8aYLrSqzAW5TOmFMc4rw87JuZG6nNSChLuwxwHo2vkcWogRB_o7Yz92LkOieQv019Y9B3_I6Omtz7jHfKChpY9j7wb6A8Ycw26LXfpCVw0MGVv0LmMYZmiV0zEE9GvoHQ4fyPvWdQk-nu4V-fPw7en-sVj__L66v10XvhIyF8rxBioObiNrKZWDEhSrhSq9miQ0oq69b7XeMF9tTA3CeNGItlbMTXbLxBX5dOzdxfA6Qsq2x-Sh69wAYUxWCV3Vutb_BbmstCmZmsDlEfQxpBShtbuIvYsHy5mdp7DzFHaews5TTIGbU_O46aH5i59-P_n66MP0hz1CtMkjDB4ajOCzbQL-q_oNxbiZUQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16489207</pqid></control><display><type>article</type><title>Bovine Lactoferrin Stimulates the Phagocytic Activity of Human Neutrophils: Identification of Its Active Domain</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Miyauchi, Hirofumi ; Hashimoto, Shin-ichi ; Nakajima, Mitsunari ; Shinoda, Ichizo ; Fukuwatari, Yasuo ; Hayasawa, Hirotoshi</creator><creatorcontrib>Miyauchi, Hirofumi ; Hashimoto, Shin-ichi ; Nakajima, Mitsunari ; Shinoda, Ichizo ; Fukuwatari, Yasuo ; Hayasawa, Hirotoshi</creatorcontrib><description>Bovine LF (bLF) at concentrations in the range of 50–250 μg/ml enhanced the phagocytic activity of human neutrophils as determined by measuring the incorporation of FITC-labeled latex beads by flow cytometry. The stimulatory effect of bLF was not abrogated by hydrolysis with pepsin. Bovine lactoferricin (bLFcin), which is a bactericidal fragment purified from a pepsin hydrolysate of bLF (bLFH), also enhanced the phagocytic activity, whereas, in contrast, the fraction of bLFH depleted of bLFcin showed no stimulatory effect. The phagocytosis-enhancing activity of bLF still remained after washing the neutrophils, following exposure to bLF. Also, bLF pretreatment of the latex beads stimulated their uptake. These results demonstrate that bLF is effective in promoting the phagocytic activity of human neutrophils. This activity appears to be due to its bLFcin domain and may involve dual mechanisms of direct binding to neutrophils and opsonin-like activity.</description><identifier>ISSN: 0008-8749</identifier><identifier>EISSN: 1090-2163</identifier><identifier>DOI: 10.1006/cimm.1997.1246</identifier><identifier>PMID: 9682001</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Bacteria - immunology ; Bacteria - metabolism ; Binding Sites ; Cattle ; Humans ; In Vitro Techniques ; Lactoferrin - analogs & derivatives ; Lactoferrin - chemistry ; Lactoferrin - metabolism ; Lactoferrin - pharmacology ; Neutrophils - drug effects ; Neutrophils - immunology ; Neutrophils - metabolism ; Peptide Fragments - chemistry ; Peptide Fragments - metabolism ; Peptide Fragments - pharmacology ; Phagocytosis - drug effects</subject><ispartof>Cellular immunology, 1998-07, Vol.187 (1), p.34-37</ispartof><rights>1998 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-7a1de41eab65667ae2e705372c7667ed355ccf88b0c4b95e39c3d3f570a67ef03</citedby><cites>FETCH-LOGICAL-c436t-7a1de41eab65667ae2e705372c7667ed355ccf88b0c4b95e39c3d3f570a67ef03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0008874997912466$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9682001$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miyauchi, Hirofumi</creatorcontrib><creatorcontrib>Hashimoto, Shin-ichi</creatorcontrib><creatorcontrib>Nakajima, Mitsunari</creatorcontrib><creatorcontrib>Shinoda, Ichizo</creatorcontrib><creatorcontrib>Fukuwatari, Yasuo</creatorcontrib><creatorcontrib>Hayasawa, Hirotoshi</creatorcontrib><title>Bovine Lactoferrin Stimulates the Phagocytic Activity of Human Neutrophils: Identification of Its Active Domain</title><title>Cellular immunology</title><addtitle>Cell Immunol</addtitle><description>Bovine LF (bLF) at concentrations in the range of 50–250 μg/ml enhanced the phagocytic activity of human neutrophils as determined by measuring the incorporation of FITC-labeled latex beads by flow cytometry. The stimulatory effect of bLF was not abrogated by hydrolysis with pepsin. Bovine lactoferricin (bLFcin), which is a bactericidal fragment purified from a pepsin hydrolysate of bLF (bLFH), also enhanced the phagocytic activity, whereas, in contrast, the fraction of bLFH depleted of bLFcin showed no stimulatory effect. The phagocytosis-enhancing activity of bLF still remained after washing the neutrophils, following exposure to bLF. Also, bLF pretreatment of the latex beads stimulated their uptake. These results demonstrate that bLF is effective in promoting the phagocytic activity of human neutrophils. This activity appears to be due to its bLFcin domain and may involve dual mechanisms of direct binding to neutrophils and opsonin-like activity.</description><subject>Animals</subject><subject>Bacteria - immunology</subject><subject>Bacteria - metabolism</subject><subject>Binding Sites</subject><subject>Cattle</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Lactoferrin - analogs & derivatives</subject><subject>Lactoferrin - chemistry</subject><subject>Lactoferrin - metabolism</subject><subject>Lactoferrin - pharmacology</subject><subject>Neutrophils - drug effects</subject><subject>Neutrophils - immunology</subject><subject>Neutrophils - metabolism</subject><subject>Peptide Fragments - chemistry</subject><subject>Peptide Fragments - metabolism</subject><subject>Peptide Fragments - pharmacology</subject><subject>Phagocytosis - drug effects</subject><issn>0008-8749</issn><issn>1090-2163</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb1v2zAUxImgReKkXbMF4NRNLilK_OiWpE1jwGgLNJ0JmnqKXyCJDkkZ8H8fCTa6FZ0eDve7G94Rcs3ZkjMmP3vs-yU3Ri15WckzsuDMsKLkUrwjC8aYLrSqzAW5TOmFMc4rw87JuZG6nNSChLuwxwHo2vkcWogRB_o7Yz92LkOieQv019Y9B3_I6Omtz7jHfKChpY9j7wb6A8Ycw26LXfpCVw0MGVv0LmMYZmiV0zEE9GvoHQ4fyPvWdQk-nu4V-fPw7en-sVj__L66v10XvhIyF8rxBioObiNrKZWDEhSrhSq9miQ0oq69b7XeMF9tTA3CeNGItlbMTXbLxBX5dOzdxfA6Qsq2x-Sh69wAYUxWCV3Vutb_BbmstCmZmsDlEfQxpBShtbuIvYsHy5mdp7DzFHaews5TTIGbU_O46aH5i59-P_n66MP0hz1CtMkjDB4ajOCzbQL-q_oNxbiZUQ</recordid><startdate>19980710</startdate><enddate>19980710</enddate><creator>Miyauchi, Hirofumi</creator><creator>Hashimoto, Shin-ichi</creator><creator>Nakajima, Mitsunari</creator><creator>Shinoda, Ichizo</creator><creator>Fukuwatari, Yasuo</creator><creator>Hayasawa, Hirotoshi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19980710</creationdate><title>Bovine Lactoferrin Stimulates the Phagocytic Activity of Human Neutrophils: Identification of Its Active Domain</title><author>Miyauchi, Hirofumi ; Hashimoto, Shin-ichi ; Nakajima, Mitsunari ; Shinoda, Ichizo ; Fukuwatari, Yasuo ; Hayasawa, Hirotoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-7a1de41eab65667ae2e705372c7667ed355ccf88b0c4b95e39c3d3f570a67ef03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Bacteria - immunology</topic><topic>Bacteria - metabolism</topic><topic>Binding Sites</topic><topic>Cattle</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Lactoferrin - analogs & derivatives</topic><topic>Lactoferrin - chemistry</topic><topic>Lactoferrin - metabolism</topic><topic>Lactoferrin - pharmacology</topic><topic>Neutrophils - drug effects</topic><topic>Neutrophils - immunology</topic><topic>Neutrophils - metabolism</topic><topic>Peptide Fragments - chemistry</topic><topic>Peptide Fragments - metabolism</topic><topic>Peptide Fragments - pharmacology</topic><topic>Phagocytosis - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miyauchi, Hirofumi</creatorcontrib><creatorcontrib>Hashimoto, Shin-ichi</creatorcontrib><creatorcontrib>Nakajima, Mitsunari</creatorcontrib><creatorcontrib>Shinoda, Ichizo</creatorcontrib><creatorcontrib>Fukuwatari, Yasuo</creatorcontrib><creatorcontrib>Hayasawa, Hirotoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miyauchi, Hirofumi</au><au>Hashimoto, Shin-ichi</au><au>Nakajima, Mitsunari</au><au>Shinoda, Ichizo</au><au>Fukuwatari, Yasuo</au><au>Hayasawa, Hirotoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bovine Lactoferrin Stimulates the Phagocytic Activity of Human Neutrophils: Identification of Its Active Domain</atitle><jtitle>Cellular immunology</jtitle><addtitle>Cell Immunol</addtitle><date>1998-07-10</date><risdate>1998</risdate><volume>187</volume><issue>1</issue><spage>34</spage><epage>37</epage><pages>34-37</pages><issn>0008-8749</issn><eissn>1090-2163</eissn><abstract>Bovine LF (bLF) at concentrations in the range of 50–250 μg/ml enhanced the phagocytic activity of human neutrophils as determined by measuring the incorporation of FITC-labeled latex beads by flow cytometry. The stimulatory effect of bLF was not abrogated by hydrolysis with pepsin. Bovine lactoferricin (bLFcin), which is a bactericidal fragment purified from a pepsin hydrolysate of bLF (bLFH), also enhanced the phagocytic activity, whereas, in contrast, the fraction of bLFH depleted of bLFcin showed no stimulatory effect. The phagocytosis-enhancing activity of bLF still remained after washing the neutrophils, following exposure to bLF. Also, bLF pretreatment of the latex beads stimulated their uptake. These results demonstrate that bLF is effective in promoting the phagocytic activity of human neutrophils. This activity appears to be due to its bLFcin domain and may involve dual mechanisms of direct binding to neutrophils and opsonin-like activity.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>9682001</pmid><doi>10.1006/cimm.1997.1246</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0008-8749 |
| ispartof | Cellular immunology, 1998-07, Vol.187 (1), p.34-37 |
| issn | 0008-8749 1090-2163 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73845858 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Bacteria - immunology Bacteria - metabolism Binding Sites Cattle Humans In Vitro Techniques Lactoferrin - analogs & derivatives Lactoferrin - chemistry Lactoferrin - metabolism Lactoferrin - pharmacology Neutrophils - drug effects Neutrophils - immunology Neutrophils - metabolism Peptide Fragments - chemistry Peptide Fragments - metabolism Peptide Fragments - pharmacology Phagocytosis - drug effects |
| title | Bovine Lactoferrin Stimulates the Phagocytic Activity of Human Neutrophils: Identification of Its Active Domain |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T13%3A25%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bovine%20Lactoferrin%20Stimulates%20the%20Phagocytic%20Activity%20of%20Human%20Neutrophils:%20Identification%20of%20Its%20Active%20Domain&rft.jtitle=Cellular%20immunology&rft.au=Miyauchi,%20Hirofumi&rft.date=1998-07-10&rft.volume=187&rft.issue=1&rft.spage=34&rft.epage=37&rft.pages=34-37&rft.issn=0008-8749&rft.eissn=1090-2163&rft_id=info:doi/10.1006/cimm.1997.1246&rft_dat=%3Cproquest_cross%3E73845858%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16489207&rft_id=info:pmid/9682001&rft_els_id=S0008874997912466&rfr_iscdi=true |