A protein inhibitor of cellulases in Dictyostelium discoideum
An inhibitor of cellulase (E.C. 3.2.1.4) was isolated from Dictyostelium discoideum in the culmination stage and partially purified. This inhibitor was shown to be a protein of molecular weight 20,000. At pH 6.5 it inhibited the two cellulases present in Dictyostelium but not cellulases from other o...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1981-10, Vol.102 (4), p.1310-1316 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Jones, Theodore H.D. Gupta, Madhu |
| description | An inhibitor of cellulase (E.C. 3.2.1.4) was isolated from
Dictyostelium
discoideum in the culmination stage and partially purified. This inhibitor was shown to be a protein of molecular weight 20,000. At pH 6.5 it inhibited the two cellulases present in
Dictyostelium but not cellulases from other organisms. Inhibition was prevented at pH 8.0 but could be recovered by adjustment of the pH to 6.5. The inhibitor was present in all stages of development from amoebae to spores but disappeared as the spores aged and was absent from germinating cells indicating that specific destruction of this inhibitor may be responsible for the activation of the cellulases. |
| doi_str_mv | 10.1016/S0006-291X(81)80154-4 |
| format | Article |
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Dictyostelium
discoideum in the culmination stage and partially purified. This inhibitor was shown to be a protein of molecular weight 20,000. At pH 6.5 it inhibited the two cellulases present in
Dictyostelium but not cellulases from other organisms. Inhibition was prevented at pH 8.0 but could be recovered by adjustment of the pH to 6.5. The inhibitor was present in all stages of development from amoebae to spores but disappeared as the spores aged and was absent from germinating cells indicating that specific destruction of this inhibitor may be responsible for the activation of the cellulases.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/S0006-291X(81)80154-4</identifier><identifier>PMID: 7317052</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>cellulase ; Cellulase - antagonists & inhibitors ; Chromatography, Gel ; Chymotrypsin - metabolism ; Dictyostelium - analysis ; Dictyostelium discoideum ; Enzyme Inhibitors - isolation & purification ; Hot Temperature ; Hydrogen-Ion Concentration ; Molecular Weight ; purification ; Trypsin - metabolism</subject><ispartof>Biochemical and biophysical research communications, 1981-10, Vol.102 (4), p.1310-1316</ispartof><rights>1981 Academic Press, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-291X(81)80154-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7317052$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jones, Theodore H.D.</creatorcontrib><creatorcontrib>Gupta, Madhu</creatorcontrib><title>A protein inhibitor of cellulases in Dictyostelium discoideum</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>An inhibitor of cellulase (E.C. 3.2.1.4) was isolated from
Dictyostelium
discoideum in the culmination stage and partially purified. This inhibitor was shown to be a protein of molecular weight 20,000. At pH 6.5 it inhibited the two cellulases present in
Dictyostelium but not cellulases from other organisms. Inhibition was prevented at pH 8.0 but could be recovered by adjustment of the pH to 6.5. The inhibitor was present in all stages of development from amoebae to spores but disappeared as the spores aged and was absent from germinating cells indicating that specific destruction of this inhibitor may be responsible for the activation of the cellulases.</description><subject>cellulase</subject><subject>Cellulase - antagonists & inhibitors</subject><subject>Chromatography, Gel</subject><subject>Chymotrypsin - metabolism</subject><subject>Dictyostelium - analysis</subject><subject>Dictyostelium discoideum</subject><subject>Enzyme Inhibitors - isolation & purification</subject><subject>Hot Temperature</subject><subject>Hydrogen-Ion Concentration</subject><subject>Molecular Weight</subject><subject>purification</subject><subject>Trypsin - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1LxDAQxYMo67r6Jyz0JHqozjTdNDmILOsnLHhQwVtIkylG-rE2rbD_vd0PvHoamPdjePMeY1OEKwQU168AIOJE4ceFxEsJOEvj9ICNERTECUJ6yMZ_yDE7CeELADEVasRGGccMZsmY3cyjVdt05OvI158-913TRk0RWSrLvjSBwrCP7rzt1k3oqPR9FTkfbOMd9dUpOypMGehsPyfs_eH-bfEUL18enxfzZUyJ4l0sjcuEFMbm0lmuhEEnksIWSSagkLl1WWGQZyKxJNyMc2M5V9IAIXBFSc4n7Hx3d_D63VPodDV4GCyampo-6IzLNJUK_wWHkFQGQgzgdA_2eUVOr1pfmXat98EM-u1Op-GtH0-tDtZTbcn5lmynXeM1gt40obdN6E3MWqLeNqFT_gsreXpW</recordid><startdate>19811030</startdate><enddate>19811030</enddate><creator>Jones, Theodore H.D.</creator><creator>Gupta, Madhu</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19811030</creationdate><title>A protein inhibitor of cellulases in Dictyostelium discoideum</title><author>Jones, Theodore H.D. ; Gupta, Madhu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e293t-8ad7686acb8dc396a1d62fcf2760f8bcd7fa13762ce6d533ac3398a0e1039e2b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>cellulase</topic><topic>Cellulase - antagonists & inhibitors</topic><topic>Chromatography, Gel</topic><topic>Chymotrypsin - metabolism</topic><topic>Dictyostelium - analysis</topic><topic>Dictyostelium discoideum</topic><topic>Enzyme Inhibitors - isolation & purification</topic><topic>Hot Temperature</topic><topic>Hydrogen-Ion Concentration</topic><topic>Molecular Weight</topic><topic>purification</topic><topic>Trypsin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jones, Theodore H.D.</creatorcontrib><creatorcontrib>Gupta, Madhu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jones, Theodore H.D.</au><au>Gupta, Madhu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A protein inhibitor of cellulases in Dictyostelium discoideum</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1981-10-30</date><risdate>1981</risdate><volume>102</volume><issue>4</issue><spage>1310</spage><epage>1316</epage><pages>1310-1316</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>An inhibitor of cellulase (E.C. 3.2.1.4) was isolated from
Dictyostelium
discoideum in the culmination stage and partially purified. This inhibitor was shown to be a protein of molecular weight 20,000. At pH 6.5 it inhibited the two cellulases present in
Dictyostelium but not cellulases from other organisms. Inhibition was prevented at pH 8.0 but could be recovered by adjustment of the pH to 6.5. The inhibitor was present in all stages of development from amoebae to spores but disappeared as the spores aged and was absent from germinating cells indicating that specific destruction of this inhibitor may be responsible for the activation of the cellulases.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7317052</pmid><doi>10.1016/S0006-291X(81)80154-4</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biochemical and biophysical research communications, 1981-10, Vol.102 (4), p.1310-1316 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73844891 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | cellulase Cellulase - antagonists & inhibitors Chromatography, Gel Chymotrypsin - metabolism Dictyostelium - analysis Dictyostelium discoideum Enzyme Inhibitors - isolation & purification Hot Temperature Hydrogen-Ion Concentration Molecular Weight purification Trypsin - metabolism |
| title | A protein inhibitor of cellulases in Dictyostelium discoideum |
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