Yellow fever 17D vaccine virus isolated from healthy vaccinees accumulates very few mutations
The live attenuated yellow fever (YF) vaccine strain 17D is one of the safest vaccines in use today with only 22 cases of reversion to virulence documented from over 300 million doses administered. We have isolated virus in cell culture from sera of six volunteers who received 17D vaccine and found...
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| Veröffentlicht in: | Virus research 1998-05, Vol.55 (1), p.93-99 |
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| creator | Xie, Hong Cass, Alvah R Barrett, Alan D.T |
| description | The live attenuated yellow fever (YF) vaccine strain 17D is one of the safest vaccines in use today with only 22 cases of reversion to virulence documented from over 300 million doses administered. We have isolated virus in cell culture from sera of six volunteers who received 17D vaccine and found that very few nucleotide mutations were detected in the consensus sequence of the entire genome of each of the serum viruses. Moreover, most of these mutations accumulated in the non-structural protein genes, especially the NS5 protein gene. Although no nucleotide change was identified in the structural protein genes of any of these six serum viruses, minor sequence heterogeneity existed in the serum virus population. Our results indicate that 17D vaccine virus accumulates mutations at a very low frequency and may explain in part the excellent safety record of 17D vaccine. |
| doi_str_mv | 10.1016/S0168-1702(98)00036-7 |
| format | Article |
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We have isolated virus in cell culture from sera of six volunteers who received 17D vaccine and found that very few nucleotide mutations were detected in the consensus sequence of the entire genome of each of the serum viruses. Moreover, most of these mutations accumulated in the non-structural protein genes, especially the NS5 protein gene. Although no nucleotide change was identified in the structural protein genes of any of these six serum viruses, minor sequence heterogeneity existed in the serum virus population. 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Our results indicate that 17D vaccine virus accumulates mutations at a very low frequency and may explain in part the excellent safety record of 17D vaccine.</description><subject>17D vaccine</subject><subject>Amino Acid Sequence</subject><subject>Amino Acid Substitution</subject><subject>Animals</subject><subject>Attenuation</subject><subject>Cercopithecus aethiops</subject><subject>Genetic Variation</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Mutations</subject><subject>Point Mutation</subject><subject>Polymerase Chain Reaction</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Vaccinees</subject><subject>Vaccines, Attenuated - blood</subject><subject>Vaccines, Attenuated - genetics</subject><subject>Vero Cells</subject><subject>Viral Nonstructural Proteins - chemistry</subject><subject>Viral Nonstructural Proteins - genetics</subject><subject>Viral Vaccines - blood</subject><subject>Viral Vaccines - genetics</subject><subject>Yellow fever virus</subject><subject>Yellow fever virus - genetics</subject><subject>Yellow fever virus - immunology</subject><subject>Yellow fever virus - isolation & purification</subject><issn>0168-1702</issn><issn>1872-7492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1uGyEURlHUKnWcPIIlVlW7mAaG4W9VVWmSRorURdpFFhFi4CITzXhcmHHktw-OXW-zAaR7-D44CC0o-UYJFZcPZVEVlaT-otVXQggTlTxBM6pkXclG1x_Q7Ih8Qmc5PxdIMClO0amWtOaUz9DTI3Td8IIDbCBhKn_ijXUurgBvYpoyjnno7AgehzT0eAm2G5fb_wxkXA5TP-2QjEvCtgS94H4a7RiHVT5HH4PtMlwc9jn6e3P95-pXdf_79u7qx33lmKZjJVirvWYqcKYYWBUIazR4SlTNQtBEhdBoyW3tdduCUJqCKMW1bpR3redsjj7vc9dp-DdBHk0fsys_sysYpmwkU03DlX4XpII3XJRXzBHfgy4NOScIZp1ib9PWUGJ2_s2bf7OTa7Qyb_5LzxwtDgVT24M_3joIL_Pv-zkUHZsIyWQXYeXAxwRuNH6I7zS8Atv2lco</recordid><startdate>19980501</startdate><enddate>19980501</enddate><creator>Xie, Hong</creator><creator>Cass, Alvah R</creator><creator>Barrett, Alan D.T</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19980501</creationdate><title>Yellow fever 17D vaccine virus isolated from healthy vaccinees accumulates very few mutations</title><author>Xie, Hong ; Cass, Alvah R ; Barrett, Alan D.T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-63b9d938f5383ea8f0349ed10823ff908ff4975a2d9bbe6891e6acc2948dcbd53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>17D vaccine</topic><topic>Amino Acid Sequence</topic><topic>Amino Acid Substitution</topic><topic>Animals</topic><topic>Attenuation</topic><topic>Cercopithecus aethiops</topic><topic>Genetic Variation</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Mutations</topic><topic>Point Mutation</topic><topic>Polymerase Chain Reaction</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Vaccinees</topic><topic>Vaccines, Attenuated - blood</topic><topic>Vaccines, Attenuated - genetics</topic><topic>Vero Cells</topic><topic>Viral Nonstructural Proteins - chemistry</topic><topic>Viral Nonstructural Proteins - genetics</topic><topic>Viral Vaccines - blood</topic><topic>Viral Vaccines - genetics</topic><topic>Yellow fever virus</topic><topic>Yellow fever virus - genetics</topic><topic>Yellow fever virus - immunology</topic><topic>Yellow fever virus - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xie, Hong</creatorcontrib><creatorcontrib>Cass, Alvah R</creatorcontrib><creatorcontrib>Barrett, Alan D.T</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virus research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xie, Hong</au><au>Cass, Alvah R</au><au>Barrett, Alan D.T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Yellow fever 17D vaccine virus isolated from healthy vaccinees accumulates very few mutations</atitle><jtitle>Virus research</jtitle><addtitle>Virus Res</addtitle><date>1998-05-01</date><risdate>1998</risdate><volume>55</volume><issue>1</issue><spage>93</spage><epage>99</epage><pages>93-99</pages><issn>0168-1702</issn><eissn>1872-7492</eissn><abstract>The live attenuated yellow fever (YF) vaccine strain 17D is one of the safest vaccines in use today with only 22 cases of reversion to virulence documented from over 300 million doses administered. 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| ispartof | Virus research, 1998-05, Vol.55 (1), p.93-99 |
| issn | 0168-1702 1872-7492 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | 17D vaccine Amino Acid Sequence Amino Acid Substitution Animals Attenuation Cercopithecus aethiops Genetic Variation Humans Molecular Sequence Data Mutation Mutations Point Mutation Polymerase Chain Reaction Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Vaccinees Vaccines, Attenuated - blood Vaccines, Attenuated - genetics Vero Cells Viral Nonstructural Proteins - chemistry Viral Nonstructural Proteins - genetics Viral Vaccines - blood Viral Vaccines - genetics Yellow fever virus Yellow fever virus - genetics Yellow fever virus - immunology Yellow fever virus - isolation & purification |
| title | Yellow fever 17D vaccine virus isolated from healthy vaccinees accumulates very few mutations |
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