Coordinated actions of RuvABC in Holliday junction processing
The RuvA, RuvB and RuvC proteins of Escherichia coli process Holliday junctions during genetic recombination and DNA repair. Biochemical studies have shown that RuvA and RuvB promote branch migration whereas RuvC resolves junctions by endonucleolytic cleavage. Here we show that RuvAB stimulate Holli...
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| Veröffentlicht in: | Journal of molecular biology 1998-08, Vol.281 (4), p.621-630 |
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| container_title | Journal of molecular biology |
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| creator | Zerbib, D Mézard, C George, H West, S C |
| description | The RuvA, RuvB and RuvC proteins of Escherichia coli process Holliday junctions during genetic recombination and DNA repair. Biochemical studies have shown that RuvA and RuvB promote branch migration whereas RuvC resolves junctions by endonucleolytic cleavage. Here we show that RuvAB stimulate Holliday junction resolution by RuvC. Elevated RuvC activity was dependent upon RuvAB-mediated ATP-hydrolysis. These results show that the three Ruv proteins work in a coordinated manner to promote Holliday junction resolution, and account for the resolvase-defective phenotype exhibited by ruvA, ruvB or ruvC mutant strains. |
| doi_str_mv | 10.1006/jmbi.1998.1959 |
| format | Article |
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Biochemical studies have shown that RuvA and RuvB promote branch migration whereas RuvC resolves junctions by endonucleolytic cleavage. Here we show that RuvAB stimulate Holliday junction resolution by RuvC. Elevated RuvC activity was dependent upon RuvAB-mediated ATP-hydrolysis. 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| ispartof | Journal of molecular biology, 1998-08, Vol.281 (4), p.621-630 |
| issn | 0022-2836 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adenosine Triphosphate - metabolism Bacterial Proteins - metabolism DNA - chemistry DNA Helicases DNA Repair - genetics DNA-Binding Proteins - metabolism Endodeoxyribonucleases - metabolism Endonucleases - metabolism Enzyme Activation - physiology Escherichia coli - physiology Escherichia coli Proteins Holliday Junction Resolvases Kinetics Nucleic Acid Conformation Recombination, Genetic - genetics |
| title | Coordinated actions of RuvABC in Holliday junction processing |
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