Chemical and Biological Properties of Subfractions of Hot-Water Extract from Delipidated BCG
MOTOMIYA, M., SATO, H., ARAI, H., NAGAI, H., ITO, M., SATOH, K., KUMANO, N. and KONNO, K. Chemical and Biological Properties of Subfractions of Hot-Water Extract from Delipidated BCG. Tohoku J. exp. Med., 1981, 133 (3), 293-300-A hot-water extract from delipidated Mycobacterium bovis strain BCG was...
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| Veröffentlicht in: | The Tohoku Journal of Experimental Medicine 1981, Vol.133(3), pp.293-300 |
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| creator | MOTOMIYA, MASAKICHI SATO, HIROSI ARAI, HIDEO NAGAI, HIROMI ITO, MASAYUKI SATOH, KEN KUMANO, NOBUKO KONNO, KIYOSHI |
| description | MOTOMIYA, M., SATO, H., ARAI, H., NAGAI, H., ITO, M., SATOH, K., KUMANO, N. and KONNO, K. Chemical and Biological Properties of Subfractions of Hot-Water Extract from Delipidated BCG. Tohoku J. exp. Med., 1981, 133 (3), 293-300-A hot-water extract from delipidated Mycobacterium bovis strain BCG was fractionated by precipitation with ethanol. The chemical and biological properties of the subfractions thus obtained were investigated. A subfraction precipitated at 20% ethanol contained less neutral sugar and more ninhydrin-positive substance than other subfractions. Only the guinea pigs that were treated with 20% ethanol-precipitate showed no humoral antibody response to egg white albumin, despite positive skin reaction. The subfractions precipitated at low concentrations of ethanol exhibited a significant antitumor activity in ddY and C3H/He mice inoculated intraperitoneally with tumor cells. These results suggest that the hot-water extract from delipidated BCG consists of subfractions with different chemical and biological properties. |
| doi_str_mv | 10.1620/tjem.133.293 |
| format | Article |
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Chemical and Biological Properties of Subfractions of Hot-Water Extract from Delipidated BCG. Tohoku J. exp. Med., 1981, 133 (3), 293-300-A hot-water extract from delipidated Mycobacterium bovis strain BCG was fractionated by precipitation with ethanol. The chemical and biological properties of the subfractions thus obtained were investigated. A subfraction precipitated at 20% ethanol contained less neutral sugar and more ninhydrin-positive substance than other subfractions. Only the guinea pigs that were treated with 20% ethanol-precipitate showed no humoral antibody response to egg white albumin, despite positive skin reaction. The subfractions precipitated at low concentrations of ethanol exhibited a significant antitumor activity in ddY and C3H/He mice inoculated intraperitoneally with tumor cells. These results suggest that the hot-water extract from delipidated BCG consists of subfractions with different chemical and biological properties.</description><identifier>ISSN: 0040-8727</identifier><identifier>EISSN: 1349-3329</identifier><identifier>DOI: 10.1620/tjem.133.293</identifier><identifier>PMID: 7314081</identifier><language>eng</language><publisher>Japan: Tohoku University Medical Press</publisher><subject>adjuvant activity ; Adjuvants, Immunologic - analysis ; Animals ; Antineoplastic Agents - analysis ; BCG Vaccine - analysis ; BCG Vaccine - immunology ; BCG Vaccine - isolation & purification ; Guinea Pigs ; immunotherapy ; Lipids ; Male ; Mice ; Mycobacterium bovis BCG ; Neoplasms, Experimental - drug therapy</subject><ispartof>The Tohoku Journal of Experimental Medicine, 1981, Vol.133(3), pp.293-300</ispartof><rights>Tohoku University Medical Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7314081$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MOTOMIYA, MASAKICHI</creatorcontrib><creatorcontrib>SATO, HIROSI</creatorcontrib><creatorcontrib>ARAI, HIDEO</creatorcontrib><creatorcontrib>NAGAI, HIROMI</creatorcontrib><creatorcontrib>ITO, MASAYUKI</creatorcontrib><creatorcontrib>SATOH, KEN</creatorcontrib><creatorcontrib>KUMANO, NOBUKO</creatorcontrib><creatorcontrib>KONNO, KIYOSHI</creatorcontrib><title>Chemical and Biological Properties of Subfractions of Hot-Water Extract from Delipidated BCG</title><title>The Tohoku Journal of Experimental Medicine</title><addtitle>Tohoku J. Exp. Med.</addtitle><description>MOTOMIYA, M., SATO, H., ARAI, H., NAGAI, H., ITO, M., SATOH, K., KUMANO, N. and KONNO, K. Chemical and Biological Properties of Subfractions of Hot-Water Extract from Delipidated BCG. Tohoku J. exp. Med., 1981, 133 (3), 293-300-A hot-water extract from delipidated Mycobacterium bovis strain BCG was fractionated by precipitation with ethanol. The chemical and biological properties of the subfractions thus obtained were investigated. A subfraction precipitated at 20% ethanol contained less neutral sugar and more ninhydrin-positive substance than other subfractions. Only the guinea pigs that were treated with 20% ethanol-precipitate showed no humoral antibody response to egg white albumin, despite positive skin reaction. The subfractions precipitated at low concentrations of ethanol exhibited a significant antitumor activity in ddY and C3H/He mice inoculated intraperitoneally with tumor cells. These results suggest that the hot-water extract from delipidated BCG consists of subfractions with different chemical and biological properties.</description><subject>adjuvant activity</subject><subject>Adjuvants, Immunologic - analysis</subject><subject>Animals</subject><subject>Antineoplastic Agents - analysis</subject><subject>BCG Vaccine - analysis</subject><subject>BCG Vaccine - immunology</subject><subject>BCG Vaccine - isolation & purification</subject><subject>Guinea Pigs</subject><subject>immunotherapy</subject><subject>Lipids</subject><subject>Male</subject><subject>Mice</subject><subject>Mycobacterium bovis BCG</subject><subject>Neoplasms, Experimental - drug therapy</subject><issn>0040-8727</issn><issn>1349-3329</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1rGzEQhkVoSZ00t1wDe-qp60oarbR7bNx8FAItJKWXgpClUbJm13IkGdp_X61tfM1lxPA-PCNeQi4ZnTPJ6Ze8wnHOAOa8gxMyYyC6GoB378iMUkHrVnH1gZyltKIUBFXylJwqYIK2bEb-LF5w7K0ZKrN21XUfhvC8W3_GsMGYe0xV8NXjdumjsbkP691-H3L922SM1c3fPAWVj2GsvuHQb3pXguJa3H0k770ZEl4c3nPy6_bmaXFfP_y4-774-lDbRqpcowXJvHLcSS49RRQNtVC-1y7BWcmhw9bwppUOVMP40nIvvWh4g5Y511o4J5_23k0Mr1tMWY99sjgMZo1hm7SCVjCh2Jsga0CUCxP4eQ_aGFKK6PUm9qOJ_zSjempdT63r0rourRf86uDdLkd0R_hQc8lv9_kqZfOMx9yUgu2AOxnrinUSwmEW8RGwLyZqXMN_sIyV2w</recordid><startdate>19810101</startdate><enddate>19810101</enddate><creator>MOTOMIYA, MASAKICHI</creator><creator>SATO, HIROSI</creator><creator>ARAI, HIDEO</creator><creator>NAGAI, HIROMI</creator><creator>ITO, MASAYUKI</creator><creator>SATOH, KEN</creator><creator>KUMANO, NOBUKO</creator><creator>KONNO, KIYOSHI</creator><general>Tohoku University Medical Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19810101</creationdate><title>Chemical and Biological Properties of Subfractions of Hot-Water Extract from Delipidated BCG</title><author>MOTOMIYA, MASAKICHI ; SATO, HIROSI ; ARAI, HIDEO ; NAGAI, HIROMI ; ITO, MASAYUKI ; SATOH, KEN ; KUMANO, NOBUKO ; KONNO, KIYOSHI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c567t-ec361f7d2d626f0ee450c30818b3dc6239e8a2586d37512bc2f6f4525ec1dd8c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>adjuvant activity</topic><topic>Adjuvants, Immunologic - analysis</topic><topic>Animals</topic><topic>Antineoplastic Agents - analysis</topic><topic>BCG Vaccine - analysis</topic><topic>BCG Vaccine - immunology</topic><topic>BCG Vaccine - isolation & purification</topic><topic>Guinea Pigs</topic><topic>immunotherapy</topic><topic>Lipids</topic><topic>Male</topic><topic>Mice</topic><topic>Mycobacterium bovis BCG</topic><topic>Neoplasms, Experimental - drug therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MOTOMIYA, MASAKICHI</creatorcontrib><creatorcontrib>SATO, HIROSI</creatorcontrib><creatorcontrib>ARAI, HIDEO</creatorcontrib><creatorcontrib>NAGAI, HIROMI</creatorcontrib><creatorcontrib>ITO, MASAYUKI</creatorcontrib><creatorcontrib>SATOH, KEN</creatorcontrib><creatorcontrib>KUMANO, NOBUKO</creatorcontrib><creatorcontrib>KONNO, KIYOSHI</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Tohoku Journal of Experimental Medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MOTOMIYA, MASAKICHI</au><au>SATO, HIROSI</au><au>ARAI, HIDEO</au><au>NAGAI, HIROMI</au><au>ITO, MASAYUKI</au><au>SATOH, KEN</au><au>KUMANO, NOBUKO</au><au>KONNO, KIYOSHI</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chemical and Biological Properties of Subfractions of Hot-Water Extract from Delipidated BCG</atitle><jtitle>The Tohoku Journal of Experimental Medicine</jtitle><addtitle>Tohoku J. Exp. Med.</addtitle><date>1981-01-01</date><risdate>1981</risdate><volume>133</volume><issue>3</issue><spage>293</spage><epage>300</epage><pages>293-300</pages><issn>0040-8727</issn><eissn>1349-3329</eissn><abstract>MOTOMIYA, M., SATO, H., ARAI, H., NAGAI, H., ITO, M., SATOH, K., KUMANO, N. and KONNO, K. Chemical and Biological Properties of Subfractions of Hot-Water Extract from Delipidated BCG. Tohoku J. exp. Med., 1981, 133 (3), 293-300-A hot-water extract from delipidated Mycobacterium bovis strain BCG was fractionated by precipitation with ethanol. The chemical and biological properties of the subfractions thus obtained were investigated. A subfraction precipitated at 20% ethanol contained less neutral sugar and more ninhydrin-positive substance than other subfractions. Only the guinea pigs that were treated with 20% ethanol-precipitate showed no humoral antibody response to egg white albumin, despite positive skin reaction. The subfractions precipitated at low concentrations of ethanol exhibited a significant antitumor activity in ddY and C3H/He mice inoculated intraperitoneally with tumor cells. These results suggest that the hot-water extract from delipidated BCG consists of subfractions with different chemical and biological properties.</abstract><cop>Japan</cop><pub>Tohoku University Medical Press</pub><pmid>7314081</pmid><doi>10.1620/tjem.133.293</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | adjuvant activity Adjuvants, Immunologic - analysis Animals Antineoplastic Agents - analysis BCG Vaccine - analysis BCG Vaccine - immunology BCG Vaccine - isolation & purification Guinea Pigs immunotherapy Lipids Male Mice Mycobacterium bovis BCG Neoplasms, Experimental - drug therapy |
| title | Chemical and Biological Properties of Subfractions of Hot-Water Extract from Delipidated BCG |
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