Target-Dependent Development of the Vesicular Acetylcholine Transporter in Rodent Sweat Gland Innervation
Descriptive studies have delineated a developmental change in neurotransmitter phenotype from noradrenergic to cholinergic in the sympathetic innervation of sweat glands in rodent footpads. Transplantation and culture experiments provide evidence that interactions with the target tissue induce this...
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Veröffentlicht in: | Developmental biology 1998-07, Vol.199 (2), p.175-184 |
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description | Descriptive studies have delineated a developmental change in neurotransmitter phenotype from noradrenergic to cholinergic in the sympathetic innervation of sweat glands in rodent footpads. Transplantation and culture experiments provide evidence that interactions with the target tissue induce this change. Recent studies with an antiserum that recognizes the vesicular acetylcholine transporter (VAChT) suggest, however, that the development of cholinergic function in sympathetic neurons, including those that innervate sweat glands, occurs prior to and does not require target contact. To clarify these apparently contradictory findings, we directly compared the appearance of VAChT immunoreactivity in the sympathetic neurons that innervate sweat glands with the time that axons contact this target. We find that VAChT immunoreactivity is not detectable in either the axons or cell bodies of sweat gland neurons until several days after target innervation. Before and during VAChT acquisition, the developing sweat gland innervation contains vesicular stores of catecholamines. An analysis of mutant mice that lack sweat glands was undertaken to determine whether VAChT expression requires target interactions and revealed that VAChT does not appear in the absence of glands. These findings, together with previous studies, confirm the target dependence of cholinergic function in the sympathetic neurons that innervate sweat glands. |
doi_str_mv | 10.1006/dbio.1998.8929 |
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Transplantation and culture experiments provide evidence that interactions with the target tissue induce this change. Recent studies with an antiserum that recognizes the vesicular acetylcholine transporter (VAChT) suggest, however, that the development of cholinergic function in sympathetic neurons, including those that innervate sweat glands, occurs prior to and does not require target contact. To clarify these apparently contradictory findings, we directly compared the appearance of VAChT immunoreactivity in the sympathetic neurons that innervate sweat glands with the time that axons contact this target. We find that VAChT immunoreactivity is not detectable in either the axons or cell bodies of sweat gland neurons until several days after target innervation. Before and during VAChT acquisition, the developing sweat gland innervation contains vesicular stores of catecholamines. An analysis of mutant mice that lack sweat glands was undertaken to determine whether VAChT expression requires target interactions and revealed that VAChT does not appear in the absence of glands. These findings, together with previous studies, confirm the target dependence of cholinergic function in the sympathetic neurons that innervate sweat glands.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1006/dbio.1998.8929</identifier><identifier>PMID: 9698438</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acetylcholine - metabolism ; Adrenergic Fibers - metabolism ; Animals ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Immunohistochemistry ; Membrane Transport Proteins ; Mice ; Mice, Mutant Strains ; Mice, Transgenic ; Rats ; Rats, Sprague-Dawley ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sweat Glands - growth & development ; Sweat Glands - innervation ; Sweat Glands - metabolism ; Sympathetic Nervous System - growth & development ; Sympathetic Nervous System - metabolism ; Vesicular Acetylcholine Transport Proteins ; Vesicular Transport Proteins</subject><ispartof>Developmental biology, 1998-07, Vol.199 (2), p.175-184</ispartof><rights>1998 Academic Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-17ef4cf4ba3755a931a97fc2ef90cb439f2b1ccf8bed517c10e76219e07433763</citedby><cites>FETCH-LOGICAL-c379t-17ef4cf4ba3755a931a97fc2ef90cb439f2b1ccf8bed517c10e76219e07433763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/dbio.1998.8929$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9698438$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guidry, Guy</creatorcontrib><creatorcontrib>Landis, Story C.</creatorcontrib><title>Target-Dependent Development of the Vesicular Acetylcholine Transporter in Rodent Sweat Gland Innervation</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>Descriptive studies have delineated a developmental change in neurotransmitter phenotype from noradrenergic to cholinergic in the sympathetic innervation of sweat glands in rodent footpads. Transplantation and culture experiments provide evidence that interactions with the target tissue induce this change. Recent studies with an antiserum that recognizes the vesicular acetylcholine transporter (VAChT) suggest, however, that the development of cholinergic function in sympathetic neurons, including those that innervate sweat glands, occurs prior to and does not require target contact. To clarify these apparently contradictory findings, we directly compared the appearance of VAChT immunoreactivity in the sympathetic neurons that innervate sweat glands with the time that axons contact this target. We find that VAChT immunoreactivity is not detectable in either the axons or cell bodies of sweat gland neurons until several days after target innervation. Before and during VAChT acquisition, the developing sweat gland innervation contains vesicular stores of catecholamines. An analysis of mutant mice that lack sweat glands was undertaken to determine whether VAChT expression requires target interactions and revealed that VAChT does not appear in the absence of glands. These findings, together with previous studies, confirm the target dependence of cholinergic function in the sympathetic neurons that innervate sweat glands.</description><subject>Acetylcholine - metabolism</subject><subject>Adrenergic Fibers - metabolism</subject><subject>Animals</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Immunohistochemistry</subject><subject>Membrane Transport Proteins</subject><subject>Mice</subject><subject>Mice, Mutant Strains</subject><subject>Mice, Transgenic</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sweat Glands - growth & development</subject><subject>Sweat Glands - innervation</subject><subject>Sweat Glands - metabolism</subject><subject>Sympathetic Nervous System - growth & development</subject><subject>Sympathetic Nervous System - metabolism</subject><subject>Vesicular Acetylcholine Transport Proteins</subject><subject>Vesicular Transport Proteins</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kDFv2zAQhYkiReK4XbsF4JRNLilKIjkaduoECFCgdYtuBEUdGwYyqZC0A__7SrGRrdMd8N69u_sQ-kLJghLSfO1aFxZUSrEQspQf0IwSWRd1U_25QDNCaFnQhjRX6DqlZ0IIE4JdokvZSFExMUNuq-NfyMUaBvAd-IzXcIA-DLupDxbnJ8C_ITmz73XESwP52Jun0DsPeBu1T0OIGSJ2Hv8IbwE_X0FnvOm17_CD9xAPOrvgP6GPVvcJPp_rHP36drdd3ReP3zcPq-VjYRiXuaAcbGVs1WrG61pLRrXk1pRgJTFtxaQtW2qMFS10NeWGEuBNSSUQXjHGGzZHt6fcIYaXPaSsdi4Z6Md7IOyT4kyMi2g1Ghcno4khpQhWDdHtdDwqStTEVk1s1cRWTWzHgZtz8r7dQfduP8McdXHSYXzv4CCqZBx4A52LYLLqgvtf9D8hcop-</recordid><startdate>19980715</startdate><enddate>19980715</enddate><creator>Guidry, Guy</creator><creator>Landis, Story C.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980715</creationdate><title>Target-Dependent Development of the Vesicular Acetylcholine Transporter in Rodent Sweat Gland Innervation</title><author>Guidry, Guy ; Landis, Story C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-17ef4cf4ba3755a931a97fc2ef90cb439f2b1ccf8bed517c10e76219e07433763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Acetylcholine - metabolism</topic><topic>Adrenergic Fibers - metabolism</topic><topic>Animals</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Immunohistochemistry</topic><topic>Membrane Transport Proteins</topic><topic>Mice</topic><topic>Mice, Mutant Strains</topic><topic>Mice, Transgenic</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sweat Glands - growth & development</topic><topic>Sweat Glands - innervation</topic><topic>Sweat Glands - metabolism</topic><topic>Sympathetic Nervous System - growth & development</topic><topic>Sympathetic Nervous System - metabolism</topic><topic>Vesicular Acetylcholine Transport Proteins</topic><topic>Vesicular Transport Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guidry, Guy</creatorcontrib><creatorcontrib>Landis, Story C.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guidry, Guy</au><au>Landis, Story C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Target-Dependent Development of the Vesicular Acetylcholine Transporter in Rodent Sweat Gland Innervation</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>1998-07-15</date><risdate>1998</risdate><volume>199</volume><issue>2</issue><spage>175</spage><epage>184</epage><pages>175-184</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><abstract>Descriptive studies have delineated a developmental change in neurotransmitter phenotype from noradrenergic to cholinergic in the sympathetic innervation of sweat glands in rodent footpads. 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An analysis of mutant mice that lack sweat glands was undertaken to determine whether VAChT expression requires target interactions and revealed that VAChT does not appear in the absence of glands. These findings, together with previous studies, confirm the target dependence of cholinergic function in the sympathetic neurons that innervate sweat glands.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9698438</pmid><doi>10.1006/dbio.1998.8929</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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source | MEDLINE; Elsevier ScienceDirect Journals Complete; Free E-Journal (出版社公開部分のみ) |
subjects | Acetylcholine - metabolism Adrenergic Fibers - metabolism Animals Carrier Proteins - genetics Carrier Proteins - metabolism Immunohistochemistry Membrane Transport Proteins Mice Mice, Mutant Strains Mice, Transgenic Rats Rats, Sprague-Dawley RNA, Messenger - genetics RNA, Messenger - metabolism Sweat Glands - growth & development Sweat Glands - innervation Sweat Glands - metabolism Sympathetic Nervous System - growth & development Sympathetic Nervous System - metabolism Vesicular Acetylcholine Transport Proteins Vesicular Transport Proteins |
title | Target-Dependent Development of the Vesicular Acetylcholine Transporter in Rodent Sweat Gland Innervation |
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