A fluorogenic method for the demonstration of human serum 5′-nucleotide phosphodiesterase isozymes after polyacrylamide gel electrophoresis
A rapid fluorogenic method for the demonstration of 5′-nucleotide phosphodiesterase in human serum has been developed. This method uses the substrate 4-methylumbelliferyl 5′-thymidylate impregnated in agarose gels or filter paper strips. Zymograms are developed in less than 30 min at 25°C, and the s...
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Veröffentlicht in: | Analytical biochemistry 1981-10, Vol.117 (1), p.24-27 |
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creator | Lo, K.W. Aoyagi, S. Tsou, K.C. |
description | A rapid fluorogenic method for the demonstration of 5′-nucleotide phosphodiesterase in human serum has been developed. This method uses the substrate 4-methylumbelliferyl 5′-thymidylate impregnated in agarose gels or filter paper strips. Zymograms are developed in less than 30 min at 25°C, and the sensitivity of this method has been compared with that of the indigogenic method. |
doi_str_mv | 10.1016/0003-2697(81)90685-0 |
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This method uses the substrate 4-methylumbelliferyl 5′-thymidylate impregnated in agarose gels or filter paper strips. Zymograms are developed in less than 30 min at 25°C, and the sensitivity of this method has been compared with that of the indigogenic method.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(81)90685-0</identifier><identifier>PMID: 6274229</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Electrophoresis, Polyacrylamide Gel - methods ; Fluorescence ; Humans ; Hymecromone - analogs & derivatives ; Hymecromone - chemical synthesis ; Isoenzymes - blood ; Phosphodiesterase I ; Phosphoric Diester Hydrolases - blood ; Umbelliferones</subject><ispartof>Analytical biochemistry, 1981-10, Vol.117 (1), p.24-27</ispartof><rights>1981</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-713888e53ecf226f0cdb4035892b57fb1b1615c7af0e7bb2cbe5bf25bc28a9923</citedby><cites>FETCH-LOGICAL-c357t-713888e53ecf226f0cdb4035892b57fb1b1615c7af0e7bb2cbe5bf25bc28a9923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(81)90685-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6274229$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lo, K.W.</creatorcontrib><creatorcontrib>Aoyagi, S.</creatorcontrib><creatorcontrib>Tsou, K.C.</creatorcontrib><title>A fluorogenic method for the demonstration of human serum 5′-nucleotide phosphodiesterase isozymes after polyacrylamide gel electrophoresis</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A rapid fluorogenic method for the demonstration of 5′-nucleotide phosphodiesterase in human serum has been developed. This method uses the substrate 4-methylumbelliferyl 5′-thymidylate impregnated in agarose gels or filter paper strips. Zymograms are developed in less than 30 min at 25°C, and the sensitivity of this method has been compared with that of the indigogenic method.</description><subject>Electrophoresis, Polyacrylamide Gel - methods</subject><subject>Fluorescence</subject><subject>Humans</subject><subject>Hymecromone - analogs & derivatives</subject><subject>Hymecromone - chemical synthesis</subject><subject>Isoenzymes - blood</subject><subject>Phosphodiesterase I</subject><subject>Phosphoric Diester Hydrolases - blood</subject><subject>Umbelliferones</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UcuKFDEUDaKM7egfKGQluijNo5NKNsIw-IIBN7oOSepmOpJU2qRKaFf-gD_jJ_klpu1mli4uF-455z7ORegpJa8oofI1IYQPTOrxhaIvNZFKDOQe2lCi5UA40ffR5o7yED1q7SshlG6FvEAXko1bxvQG_brCIa2llluYo8cZll2ZcCgVLzvAE-Qyt6XaJZYZl4B3a7YzblDXjMWfn7-HefUJyhInwPtdaT2mCG2Bahvg2MqPQ4aGbegVvC_pYH09JJuP_FtIGBL4pZYuq9Bie4weBJsaPDnnS_Tl3dvP1x-Gm0_vP15f3Qyei3EZRsqVUiA4-MCYDMRPbku4UJo5MQZHHZVU-NEGAqNzzDsQLjDhPFNWa8Yv0fNT330t39a-r8mxeUjJzlDWZkauuGRUd-L2RPS1tFYhmH2N2daDocQcv2COFpujxUZR8-8LhnTZs3P_1WWY7kRn2zv-5oRDP_J7hGqajzB7mGLthpipxP8P-Au_zZvo</recordid><startdate>198110</startdate><enddate>198110</enddate><creator>Lo, K.W.</creator><creator>Aoyagi, S.</creator><creator>Tsou, K.C.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198110</creationdate><title>A fluorogenic method for the demonstration of human serum 5′-nucleotide phosphodiesterase isozymes after polyacrylamide gel electrophoresis</title><author>Lo, K.W. ; Aoyagi, S. ; Tsou, K.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-713888e53ecf226f0cdb4035892b57fb1b1615c7af0e7bb2cbe5bf25bc28a9923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Electrophoresis, Polyacrylamide Gel - methods</topic><topic>Fluorescence</topic><topic>Humans</topic><topic>Hymecromone - analogs & derivatives</topic><topic>Hymecromone - chemical synthesis</topic><topic>Isoenzymes - blood</topic><topic>Phosphodiesterase I</topic><topic>Phosphoric Diester Hydrolases - blood</topic><topic>Umbelliferones</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lo, K.W.</creatorcontrib><creatorcontrib>Aoyagi, S.</creatorcontrib><creatorcontrib>Tsou, K.C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lo, K.W.</au><au>Aoyagi, S.</au><au>Tsou, K.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A fluorogenic method for the demonstration of human serum 5′-nucleotide phosphodiesterase isozymes after polyacrylamide gel electrophoresis</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1981-10</date><risdate>1981</risdate><volume>117</volume><issue>1</issue><spage>24</spage><epage>27</epage><pages>24-27</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>A rapid fluorogenic method for the demonstration of 5′-nucleotide phosphodiesterase in human serum has been developed. This method uses the substrate 4-methylumbelliferyl 5′-thymidylate impregnated in agarose gels or filter paper strips. Zymograms are developed in less than 30 min at 25°C, and the sensitivity of this method has been compared with that of the indigogenic method.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6274229</pmid><doi>10.1016/0003-2697(81)90685-0</doi><tpages>4</tpages></addata></record> |
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subjects | Electrophoresis, Polyacrylamide Gel - methods Fluorescence Humans Hymecromone - analogs & derivatives Hymecromone - chemical synthesis Isoenzymes - blood Phosphodiesterase I Phosphoric Diester Hydrolases - blood Umbelliferones |
title | A fluorogenic method for the demonstration of human serum 5′-nucleotide phosphodiesterase isozymes after polyacrylamide gel electrophoresis |
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