Destabilized inheritance of pSC101 and other Escherichia coli plasmids by DpiA, a novel two‐component system regulator
We identified a gene (dpiAdestabilizer of plasmid inheritance) which, when overexpressed in Escherichia coli, destabilizes the inheritance of pSC101 and other iteron‐containing plasmids as disparate as mini‐F and RK6 but not the inheritance of P1, RSF1010 and ColD. These effects of DpiA, which funct...
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| Veröffentlicht in: | Molecular microbiology 1998-07, Vol.29 (1), p.49-59 |
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| creator | Ingmer, Hanne Miller, Christine A. Cohen, Stanley N. |
| description | We identified a gene (dpiAdestabilizer of plasmid inheritance) which, when overexpressed in Escherichia coli, destabilizes the inheritance of pSC101 and other iteron‐containing plasmids as disparate as mini‐F and RK6 but not the inheritance of P1, RSF1010 and ColD. These effects of DpiA, which functions like an effector protein for a previously undescribed two‐component signal transduction system, were reduced by mutations known to promote pSC101 replication and partitioning. dpiB, a gene encoding the putative histidine kinase of this two‐component system, is located immediately 5′ to dpiA and adjacent to a DpiA‐induced target promoter that transcribes genes having homology to citrate lyase operon genes, citC, citD and citE, of Klebsiella pneumoniae. Disruption of dpiB reversed or reduced the effect of DpiA overproduction on pSC101 inheritance. A second DpiA target, the promoter for a gene (appY ) implicated in E. coli's response to anaerobiosis, is repressed by DpiA. A mutation in dpiA at a site commonly conserved and phosphorylated in two‐component system effector proteins abolished the effects of DpiA overproduction on pSC101 inheritance and negative regulation of appY expression. Our findings suggest a possible mechanism by which environmental and/or cellular stimuli may influence plasmid inheritance. |
| doi_str_mv | 10.1046/j.1365-2958.1998.00895.x |
| format | Article |
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These effects of DpiA, which functions like an effector protein for a previously undescribed two‐component signal transduction system, were reduced by mutations known to promote pSC101 replication and partitioning. dpiB, a gene encoding the putative histidine kinase of this two‐component system, is located immediately 5′ to dpiA and adjacent to a DpiA‐induced target promoter that transcribes genes having homology to citrate lyase operon genes, citC, citD and citE, of Klebsiella pneumoniae. Disruption of dpiB reversed or reduced the effect of DpiA overproduction on pSC101 inheritance. A second DpiA target, the promoter for a gene (appY ) implicated in E. coli's response to anaerobiosis, is repressed by DpiA. A mutation in dpiA at a site commonly conserved and phosphorylated in two‐component system effector proteins abolished the effects of DpiA overproduction on pSC101 inheritance and negative regulation of appY expression. Our findings suggest a possible mechanism by which environmental and/or cellular stimuli may influence plasmid inheritance.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1046/j.1365-2958.1998.00895.x</identifier><identifier>PMID: 9701802</identifier><language>eng</language><publisher>Oxford BSL: Blackwell Science Ltd, UK</publisher><subject>Amino Acid Sequence ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Base Sequence ; Chromosome Mapping ; Chromosomes, Bacterial ; DNA, Bacterial ; Escherichia coli Proteins ; Gene Expression Regulation, Bacterial ; Genes, Bacterial ; Histidine Kinase ; Molecular Sequence Data ; Mutation ; Phosphorylation ; Plasmids ; Protein Kinases - genetics ; Protein Kinases - metabolism ; Sequence Analysis, DNA ; Transcription Factors</subject><ispartof>Molecular microbiology, 1998-07, Vol.29 (1), p.49-59</ispartof><rights>Blackwell Science Ltd, Oxford</rights><rights>Copyright Blackwell Scientific Publications Ltd. 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These effects of DpiA, which functions like an effector protein for a previously undescribed two‐component signal transduction system, were reduced by mutations known to promote pSC101 replication and partitioning. dpiB, a gene encoding the putative histidine kinase of this two‐component system, is located immediately 5′ to dpiA and adjacent to a DpiA‐induced target promoter that transcribes genes having homology to citrate lyase operon genes, citC, citD and citE, of Klebsiella pneumoniae. Disruption of dpiB reversed or reduced the effect of DpiA overproduction on pSC101 inheritance. A second DpiA target, the promoter for a gene (appY ) implicated in E. coli's response to anaerobiosis, is repressed by DpiA. A mutation in dpiA at a site commonly conserved and phosphorylated in two‐component system effector proteins abolished the effects of DpiA overproduction on pSC101 inheritance and negative regulation of appY expression. Our findings suggest a possible mechanism by which environmental and/or cellular stimuli may influence plasmid inheritance.</description><subject>Amino Acid Sequence</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Base Sequence</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Bacterial</subject><subject>DNA, Bacterial</subject><subject>Escherichia coli Proteins</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes, Bacterial</subject><subject>Histidine Kinase</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Phosphorylation</subject><subject>Plasmids</subject><subject>Protein Kinases - genetics</subject><subject>Protein Kinases - metabolism</subject><subject>Sequence Analysis, DNA</subject><subject>Transcription Factors</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFu1DAQhi0EKkvhEZAsDpxI8MSxY0tcqm2BSq04ABI3y3FmqVdJHOyk3eXEI_CMPAkJu-qBC5xmpP-bXxp9hFBgObBSvt7mwKXICi1UDlqrnDGlRb57QFb3wUOyYlqwjKviy2PyJKUtY8CZ5CfkRFcMFCtWZHeOabS1b_13bKjvbzD60fYOadjQ4eMaGFDbNzSMc0IvklsAd-MtdaH1dGht6nyTaL2n54M_e0Ut7cMttnS8C79-_HShG0KP_UjTPo3Y0Yhfp9aOIT4ljza2TfjsOE_J57cXn9bvs6sP7y7XZ1eZKxkX2caCdo2tCpDolOPYyJIL0AiIaLWUjFssUOi6VLIRolAAUnGp6soqtA0_JS8PvUMM36b5WdP55LBtbY9hSqbiijPGxD9BkKViQlcz-OIvcBum2M9PGNBSQAFazZA6QC6GlCJuzBB9Z-PeADOLQrM1iymzmDKLQvNHodnNp8-P_VPdYXN_eHQ2528O-Z1vcf_fveb6-nJe-G8wAqtR</recordid><startdate>199807</startdate><enddate>199807</enddate><creator>Ingmer, Hanne</creator><creator>Miller, Christine A.</creator><creator>Cohen, Stanley N.</creator><general>Blackwell Science Ltd, UK</general><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199807</creationdate><title>Destabilized inheritance of pSC101 and other Escherichia coli plasmids by DpiA, a novel two‐component system regulator</title><author>Ingmer, Hanne ; 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These effects of DpiA, which functions like an effector protein for a previously undescribed two‐component signal transduction system, were reduced by mutations known to promote pSC101 replication and partitioning. dpiB, a gene encoding the putative histidine kinase of this two‐component system, is located immediately 5′ to dpiA and adjacent to a DpiA‐induced target promoter that transcribes genes having homology to citrate lyase operon genes, citC, citD and citE, of Klebsiella pneumoniae. Disruption of dpiB reversed or reduced the effect of DpiA overproduction on pSC101 inheritance. A second DpiA target, the promoter for a gene (appY ) implicated in E. coli's response to anaerobiosis, is repressed by DpiA. A mutation in dpiA at a site commonly conserved and phosphorylated in two‐component system effector proteins abolished the effects of DpiA overproduction on pSC101 inheritance and negative regulation of appY expression. 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| ispartof | Molecular microbiology, 1998-07, Vol.29 (1), p.49-59 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence Chromosome Mapping Chromosomes, Bacterial DNA, Bacterial Escherichia coli Proteins Gene Expression Regulation, Bacterial Genes, Bacterial Histidine Kinase Molecular Sequence Data Mutation Phosphorylation Plasmids Protein Kinases - genetics Protein Kinases - metabolism Sequence Analysis, DNA Transcription Factors |
| title | Destabilized inheritance of pSC101 and other Escherichia coli plasmids by DpiA, a novel two‐component system regulator |
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