DIURESIS FROM STIMULATION OF LEFT ATRIAL RECEPTORS: INITIAL PURIFICATION STEPS FROM PLASMA OF THE CAUSATIVE AGENT
This paper reports the initial purification steps of a humoral agent which may mediate the diuresis resulting from stimulation of left atrial receptors in anaesthetized dogs. The agent was shown to be stabilized in plasma by acidification to pH 3·2. Acid acetone precipitation was found to be a conv...
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| Veröffentlicht in: | Experimental physiology 1981-10, Vol.66 (4), p.439-445 |
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| container_title | Experimental physiology |
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| creator | Knapp, Meryl F. Linden, R. J. Pearson, M. Joan Pither, J. M. Whitaker, Elaine M. |
| description | This paper reports the initial purification steps of a humoral agent which may mediate the diuresis resulting from stimulation
of left atrial receptors in anaesthetized dogs. The agent was shown to be stabilized in plasma by acidification to pH 3·2.
Acid acetone precipitation was found to be a convenient method as a first stage in the purification of the humoral agent in
plasma. Heat stability studies showed that the agent was stable in acidified plasma for up to 20 min of heating in a boiling
water bath. Precipitation of plasma proteins under these conditions made this another possible first stage in the purification
of the agent in plasma. Some general conclusions about the possible nature of the humoral agent are discussed. |
| doi_str_mv | 10.1113/expphysiol.1981.sp002586 |
| format | Article |
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of left atrial receptors in anaesthetized dogs. The agent was shown to be stabilized in plasma by acidification to pH 3·2.
Acid acetone precipitation was found to be a convenient method as a first stage in the purification of the humoral agent in
plasma. Heat stability studies showed that the agent was stable in acidified plasma for up to 20 min of heating in a boiling
water bath. Precipitation of plasma proteins under these conditions made this another possible first stage in the purification
of the agent in plasma. Some general conclusions about the possible nature of the humoral agent are discussed.</description><identifier>ISSN: 0958-0670</identifier><identifier>ISSN: 0144-8757</identifier><identifier>EISSN: 1469-445X</identifier><identifier>DOI: 10.1113/expphysiol.1981.sp002586</identifier><identifier>PMID: 6914678</identifier><language>eng</language><publisher>England: The Physiological Society</publisher><subject>Animals ; Blood Physiological Phenomena ; Diuresis ; Dogs ; Electric Stimulation ; Heart Atria - innervation ; Hot Temperature ; Hydrogen-Ion Concentration ; Sensory Receptor Cells - physiology</subject><ispartof>Experimental physiology, 1981-10, Vol.66 (4), p.439-445</ispartof><rights>1981 The Physiological Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4519-7c9ef14d051494501d54d716f5e614f3a9e1b82d265e636e7fb3c92e92f6c6e73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6914678$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Knapp, Meryl F.</creatorcontrib><creatorcontrib>Linden, R. J.</creatorcontrib><creatorcontrib>Pearson, M. Joan</creatorcontrib><creatorcontrib>Pither, J. M.</creatorcontrib><creatorcontrib>Whitaker, Elaine M.</creatorcontrib><title>DIURESIS FROM STIMULATION OF LEFT ATRIAL RECEPTORS: INITIAL PURIFICATION STEPS FROM PLASMA OF THE CAUSATIVE AGENT</title><title>Experimental physiology</title><addtitle>Q J Exp Physiol</addtitle><description>This paper reports the initial purification steps of a humoral agent which may mediate the diuresis resulting from stimulation
of left atrial receptors in anaesthetized dogs. The agent was shown to be stabilized in plasma by acidification to pH 3·2.
Acid acetone precipitation was found to be a convenient method as a first stage in the purification of the humoral agent in
plasma. Heat stability studies showed that the agent was stable in acidified plasma for up to 20 min of heating in a boiling
water bath. Precipitation of plasma proteins under these conditions made this another possible first stage in the purification
of the agent in plasma. Some general conclusions about the possible nature of the humoral agent are discussed.</description><subject>Animals</subject><subject>Blood Physiological Phenomena</subject><subject>Diuresis</subject><subject>Dogs</subject><subject>Electric Stimulation</subject><subject>Heart Atria - innervation</subject><subject>Hot Temperature</subject><subject>Hydrogen-Ion Concentration</subject><subject>Sensory Receptor Cells - physiology</subject><issn>0958-0670</issn><issn>0144-8757</issn><issn>1469-445X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkFFvmzAUha1pVZu2-wmT_LQ3MhuMwXtDzDSWSEBgqr1ZCZiGiQyCG3X59wWRtq99snzud87VPQBAjJYYY-en_t_3-7NpunaJmY-XpkfIdn36BSwwocwixP3zFSwQc30LUQ_dgFtj_iKEHeSTa3BN2Yh5_gIcf4si47nIYZQla5hLsS7iQIpkA5MIxjySMJCZCGKY8ZCnMsnyX1BshJyktMhEJMIZzyVPLylpHOTrYAqQKw7DoMhH5JHD4IFv5D24qret0d8u7x0oIi7DlRUnD2NWbJXExczySqZrTCrkYsKIi3DlksrDtHY1xaR2tkzjnW9XNh0Fh2qv3jklszWza1qOX-cO_Jhz-6E7nrR5VofGlLptt_90dzLKc3zkMW8C_Rksh86YQdeqH5rDdjgrjNTUtvpoW01tq7e2R-v3y47T7qCrd-Ol3nEezvOXptXnT-cqnq4mgVJCHPZxyb552r80g1azy3Rlo5_HbVQRNYGvYWCY9g</recordid><startdate>19811010</startdate><enddate>19811010</enddate><creator>Knapp, Meryl F.</creator><creator>Linden, R. J.</creator><creator>Pearson, M. Joan</creator><creator>Pither, J. M.</creator><creator>Whitaker, Elaine M.</creator><general>The Physiological Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19811010</creationdate><title>DIURESIS FROM STIMULATION OF LEFT ATRIAL RECEPTORS: INITIAL PURIFICATION STEPS FROM PLASMA OF THE CAUSATIVE AGENT</title><author>Knapp, Meryl F. ; Linden, R. J. ; Pearson, M. Joan ; Pither, J. M. ; Whitaker, Elaine M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4519-7c9ef14d051494501d54d716f5e614f3a9e1b82d265e636e7fb3c92e92f6c6e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Animals</topic><topic>Blood Physiological Phenomena</topic><topic>Diuresis</topic><topic>Dogs</topic><topic>Electric Stimulation</topic><topic>Heart Atria - innervation</topic><topic>Hot Temperature</topic><topic>Hydrogen-Ion Concentration</topic><topic>Sensory Receptor Cells - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Knapp, Meryl F.</creatorcontrib><creatorcontrib>Linden, R. J.</creatorcontrib><creatorcontrib>Pearson, M. Joan</creatorcontrib><creatorcontrib>Pither, J. M.</creatorcontrib><creatorcontrib>Whitaker, Elaine M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Knapp, Meryl F.</au><au>Linden, R. J.</au><au>Pearson, M. Joan</au><au>Pither, J. M.</au><au>Whitaker, Elaine M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DIURESIS FROM STIMULATION OF LEFT ATRIAL RECEPTORS: INITIAL PURIFICATION STEPS FROM PLASMA OF THE CAUSATIVE AGENT</atitle><jtitle>Experimental physiology</jtitle><addtitle>Q J Exp Physiol</addtitle><date>1981-10-10</date><risdate>1981</risdate><volume>66</volume><issue>4</issue><spage>439</spage><epage>445</epage><pages>439-445</pages><issn>0958-0670</issn><issn>0144-8757</issn><eissn>1469-445X</eissn><abstract>This paper reports the initial purification steps of a humoral agent which may mediate the diuresis resulting from stimulation
of left atrial receptors in anaesthetized dogs. The agent was shown to be stabilized in plasma by acidification to pH 3·2.
Acid acetone precipitation was found to be a convenient method as a first stage in the purification of the humoral agent in
plasma. Heat stability studies showed that the agent was stable in acidified plasma for up to 20 min of heating in a boiling
water bath. Precipitation of plasma proteins under these conditions made this another possible first stage in the purification
of the agent in plasma. Some general conclusions about the possible nature of the humoral agent are discussed.</abstract><cop>England</cop><pub>The Physiological Society</pub><pmid>6914678</pmid><doi>10.1113/expphysiol.1981.sp002586</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0958-0670 |
| ispartof | Experimental physiology, 1981-10, Vol.66 (4), p.439-445 |
| issn | 0958-0670 0144-8757 1469-445X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73807977 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Blood Physiological Phenomena Diuresis Dogs Electric Stimulation Heart Atria - innervation Hot Temperature Hydrogen-Ion Concentration Sensory Receptor Cells - physiology |
| title | DIURESIS FROM STIMULATION OF LEFT ATRIAL RECEPTORS: INITIAL PURIFICATION STEPS FROM PLASMA OF THE CAUSATIVE AGENT |
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