The effect of lymphocyte recovery on lymphocyte typing results
This study examines the relationship between percent recovery of lymphocytes and T- and B-cell typing results. Lymphocytes were recovered from heparinized whole blood by density gradient centrifugation. T- and B-cells were enumerated by spontaneous sheep rosetting and direct fluorescent antibody sta...
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| Veröffentlicht in: | American journal of clinical pathology 1981-12, Vol.76 (6), p.745-752 |
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| container_end_page | 752 |
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| container_issue | 6 |
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| container_title | American journal of clinical pathology |
| container_volume | 76 |
| creator | Hicks, M J Jones, J F Thies, A C Minnich, L L |
| description | This study examines the relationship between percent recovery of lymphocytes and T- and B-cell typing results. Lymphocytes were recovered from heparinized whole blood by density gradient centrifugation. T- and B-cells were enumerated by spontaneous sheep rosetting and direct fluorescent antibody staining for surface immunoglobulin, respectively. The findings indicate that at low recovery levels ( less than 80%) there is a significant increase in variability of lymphocyte typing results and a significant increase in IgG-bearing B-lymphocytes. Possible explanations for these findings are discussed. These results suggest that lymphocyte typing results should be interpreted in relation to recovery level and that recovery levels of greater than or equal to 80% are desirable. |
| doi_str_mv | 10.1093/ajcp/76.6.745 |
| format | Article |
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Lymphocytes were recovered from heparinized whole blood by density gradient centrifugation. T- and B-cells were enumerated by spontaneous sheep rosetting and direct fluorescent antibody staining for surface immunoglobulin, respectively. The findings indicate that at low recovery levels ( less than 80%) there is a significant increase in variability of lymphocyte typing results and a significant increase in IgG-bearing B-lymphocytes. Possible explanations for these findings are discussed. These results suggest that lymphocyte typing results should be interpreted in relation to recovery level and that recovery levels of greater than or equal to 80% are desirable.</description><identifier>ISSN: 0002-9173</identifier><identifier>EISSN: 1943-7722</identifier><identifier>DOI: 10.1093/ajcp/76.6.745</identifier><identifier>PMID: 7315792</identifier><language>eng</language><publisher>England</publisher><subject>Adolescent ; Adult ; Aged ; Cell Separation - methods ; Child ; Child, Preschool ; Humans ; Infant ; Infant, Newborn ; Leukocyte Count ; Lymphocytes ; Middle Aged ; Receptors, Antigen, B-Cell - analysis ; Rosette Formation ; Statistics as Topic</subject><ispartof>American journal of clinical pathology, 1981-12, Vol.76 (6), p.745-752</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c288t-1b1a84a76b0db42bf554ef67087c7a86ec99d534c25e84b5577ef150d5d18e143</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7315792$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hicks, M J</creatorcontrib><creatorcontrib>Jones, J F</creatorcontrib><creatorcontrib>Thies, A C</creatorcontrib><creatorcontrib>Minnich, L L</creatorcontrib><title>The effect of lymphocyte recovery on lymphocyte typing results</title><title>American journal of clinical pathology</title><addtitle>Am J Clin Pathol</addtitle><description>This study examines the relationship between percent recovery of lymphocytes and T- and B-cell typing results. Lymphocytes were recovered from heparinized whole blood by density gradient centrifugation. T- and B-cells were enumerated by spontaneous sheep rosetting and direct fluorescent antibody staining for surface immunoglobulin, respectively. The findings indicate that at low recovery levels ( less than 80%) there is a significant increase in variability of lymphocyte typing results and a significant increase in IgG-bearing B-lymphocytes. Possible explanations for these findings are discussed. These results suggest that lymphocyte typing results should be interpreted in relation to recovery level and that recovery levels of greater than or equal to 80% are desirable.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Cell Separation - methods</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Leukocyte Count</subject><subject>Lymphocytes</subject><subject>Middle Aged</subject><subject>Receptors, Antigen, B-Cell - analysis</subject><subject>Rosette Formation</subject><subject>Statistics as Topic</subject><issn>0002-9173</issn><issn>1943-7722</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkEtLw0AUhQdRaq0uXQpZuUs77zvZCFLqAwpu6npIJndsS9LEmUTIvzelRVwdOOfjLD5C7hmdM5qJRb537QL0XM9BqgsyZZkUKQDnl2RKKeVpxkBck5sY95QybqickAkIpiDjU_K02WKC3qPrksYn1VC328YNHSYBXfODYUiaw_-6G9rd4WtcY1918ZZc-byKeHfOGfl8WW2Wb-n64_V9-bxOHTemS1nBciNz0AUtC8kLr5REr4EacJAbjS7LSiWk4wqNLJQCQM8ULVXJDDIpZuTx9NuG5rvH2Nl6Fx1WVX7Apo8WBJhMKzaC6Ql0oYkxoLdt2NV5GCyj9ujLHn1Z0Fbb0dfIP5yP-6LG8o8-CxK_ovpm4g</recordid><startdate>198112</startdate><enddate>198112</enddate><creator>Hicks, M J</creator><creator>Jones, J F</creator><creator>Thies, A C</creator><creator>Minnich, L L</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198112</creationdate><title>The effect of lymphocyte recovery on lymphocyte typing results</title><author>Hicks, M J ; Jones, J F ; Thies, A C ; Minnich, L L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c288t-1b1a84a76b0db42bf554ef67087c7a86ec99d534c25e84b5577ef150d5d18e143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Cell Separation - methods</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Leukocyte Count</topic><topic>Lymphocytes</topic><topic>Middle Aged</topic><topic>Receptors, Antigen, B-Cell - analysis</topic><topic>Rosette Formation</topic><topic>Statistics as Topic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hicks, M J</creatorcontrib><creatorcontrib>Jones, J F</creatorcontrib><creatorcontrib>Thies, A C</creatorcontrib><creatorcontrib>Minnich, L L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hicks, M J</au><au>Jones, J F</au><au>Thies, A C</au><au>Minnich, L L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of lymphocyte recovery on lymphocyte typing results</atitle><jtitle>American journal of clinical pathology</jtitle><addtitle>Am J Clin Pathol</addtitle><date>1981-12</date><risdate>1981</risdate><volume>76</volume><issue>6</issue><spage>745</spage><epage>752</epage><pages>745-752</pages><issn>0002-9173</issn><eissn>1943-7722</eissn><abstract>This study examines the relationship between percent recovery of lymphocytes and T- and B-cell typing results. Lymphocytes were recovered from heparinized whole blood by density gradient centrifugation. T- and B-cells were enumerated by spontaneous sheep rosetting and direct fluorescent antibody staining for surface immunoglobulin, respectively. The findings indicate that at low recovery levels ( less than 80%) there is a significant increase in variability of lymphocyte typing results and a significant increase in IgG-bearing B-lymphocytes. Possible explanations for these findings are discussed. These results suggest that lymphocyte typing results should be interpreted in relation to recovery level and that recovery levels of greater than or equal to 80% are desirable.</abstract><cop>England</cop><pmid>7315792</pmid><doi>10.1093/ajcp/76.6.745</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0002-9173 |
| ispartof | American journal of clinical pathology, 1981-12, Vol.76 (6), p.745-752 |
| issn | 0002-9173 1943-7722 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73789651 |
| source | MEDLINE; Oxford University Press Archive |
| subjects | Adolescent Adult Aged Cell Separation - methods Child Child, Preschool Humans Infant Infant, Newborn Leukocyte Count Lymphocytes Middle Aged Receptors, Antigen, B-Cell - analysis Rosette Formation Statistics as Topic |
| title | The effect of lymphocyte recovery on lymphocyte typing results |
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