High-Performance Liquid Chromatographic Analysis of Iodochlorhydroxyquin in Plasma
A simple isocratic high-performance liquid chromatographic procedure for the analysis of iodochlorhydroxyquin in human plasma is described. Protein was precipitated using perchloric acid, and the supernatant and precipitated protein fractions were extracted with ether. The ether phases were evaporat...
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| Veröffentlicht in: | Journal of pharmaceutical sciences 1981-08, Vol.70 (8), p.889-891 |
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| container_title | Journal of pharmaceutical sciences |
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| creator | Ezzedeen, F.W. Masoud, A.N. Stohs, S.J. |
| description | A simple isocratic high-performance liquid chromatographic procedure for the analysis of iodochlorhydroxyquin in human plasma is described. Protein was precipitated using perchloric acid, and the supernatant and precipitated protein fractions were extracted with ether. The ether phases were evaporated to dryness, reconstituted in mobile phase, and chromatographed. A reversed-phase microparticulate C18 column, a precolumn, and a UV detector at 256 nm were used. A mobile phase containing 80% methanol and 20% 0.05 M phosphoric acid was employed at a flow rate of 1 ml/min. Quantitation of iodochlorhydroxyquin in the 1-15-μg/ml range in human plasma was demonstrated with a coefficient of variance of 0.1-0.06. Hydrocortisone, which is used in combination with iodochlorhydroxyquin in ointments and creams, does not interfere in the assay. |
| doi_str_mv | 10.1002/jps.2600700814 |
| format | Article |
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Protein was precipitated using perchloric acid, and the supernatant and precipitated protein fractions were extracted with ether. The ether phases were evaporated to dryness, reconstituted in mobile phase, and chromatographed. A reversed-phase microparticulate C18 column, a precolumn, and a UV detector at 256 nm were used. A mobile phase containing 80% methanol and 20% 0.05 M phosphoric acid was employed at a flow rate of 1 ml/min. Quantitation of iodochlorhydroxyquin in the 1-15-μg/ml range in human plasma was demonstrated with a coefficient of variance of 0.1-0.06. Hydrocortisone, which is used in combination with iodochlorhydroxyquin in ointments and creams, does not interfere in the assay.</description><identifier>ISSN: 0022-3549</identifier><identifier>EISSN: 1520-6017</identifier><identifier>DOI: 10.1002/jps.2600700814</identifier><identifier>PMID: 6458695</identifier><language>eng</language><publisher>Washington: Elsevier Inc</publisher><subject>Anti-infectives-iodochlorhydroxyquin ; Anti-infectives—iodochlorhydroxyquin, high-performance liquid chromatographic analysis, plasma ; Chromatography, High Pressure Liquid ; Clioquinol - blood ; high-performance liquid chromatographic analysis ; High-performance liquid chromatography-analysis ; High-performance liquid chromatography—analysis, iodochlorhydroxyquin, plasma ; Humans ; iodochlorhydroxyquin ; Iodochlorhydroxyquin-high-performance liquid chromatographic analysis ; Iodochlorhydroxyquin—high-performance liquid chromatographic analysis, plasma ; plasma</subject><ispartof>Journal of pharmaceutical sciences, 1981-08, Vol.70 (8), p.889-891</ispartof><rights>1981 Wiley-Liss, Inc., A Wiley Company</rights><rights>Copyright © 1981 Wiley‐Liss, Inc., A Wiley Company</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4274-412653da24f4834c46ac44f704080fa312de5cc34a029e54c914d1490825e8fd3</citedby><cites>FETCH-LOGICAL-c4274-412653da24f4834c46ac44f704080fa312de5cc34a029e54c914d1490825e8fd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjps.2600700814$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjps.2600700814$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6458695$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ezzedeen, F.W.</creatorcontrib><creatorcontrib>Masoud, A.N.</creatorcontrib><creatorcontrib>Stohs, S.J.</creatorcontrib><title>High-Performance Liquid Chromatographic Analysis of Iodochlorhydroxyquin in Plasma</title><title>Journal of pharmaceutical sciences</title><addtitle>J. Pharm. Sci</addtitle><description>A simple isocratic high-performance liquid chromatographic procedure for the analysis of iodochlorhydroxyquin in human plasma is described. Protein was precipitated using perchloric acid, and the supernatant and precipitated protein fractions were extracted with ether. The ether phases were evaporated to dryness, reconstituted in mobile phase, and chromatographed. A reversed-phase microparticulate C18 column, a precolumn, and a UV detector at 256 nm were used. A mobile phase containing 80% methanol and 20% 0.05 M phosphoric acid was employed at a flow rate of 1 ml/min. Quantitation of iodochlorhydroxyquin in the 1-15-μg/ml range in human plasma was demonstrated with a coefficient of variance of 0.1-0.06. Hydrocortisone, which is used in combination with iodochlorhydroxyquin in ointments and creams, does not interfere in the assay.</description><subject>Anti-infectives-iodochlorhydroxyquin</subject><subject>Anti-infectives—iodochlorhydroxyquin, high-performance liquid chromatographic analysis, plasma</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Clioquinol - blood</subject><subject>high-performance liquid chromatographic analysis</subject><subject>High-performance liquid chromatography-analysis</subject><subject>High-performance liquid chromatography—analysis, iodochlorhydroxyquin, plasma</subject><subject>Humans</subject><subject>iodochlorhydroxyquin</subject><subject>Iodochlorhydroxyquin-high-performance liquid chromatographic analysis</subject><subject>Iodochlorhydroxyquin—high-performance liquid chromatographic analysis, plasma</subject><subject>plasma</subject><issn>0022-3549</issn><issn>1520-6017</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtPGzEURi3UiobHll2lWXU36bV9PY8liiDQRm1UqLq0jO1hDDNxsBNg_n2NJqJigSpZ8uKe75PvMSEnFKYUgH29W8cpKwBKgIriHplQwSAvgJYfyCQBLOcC60_kIMY7AChAiH2yX6CoilpMyK8Ld9vmSxsaH3q10jZbuIetM9msDb5XG38b1Lp1OjtdqW6ILma-yS698brtfGgHE_zzkAKrLJ1lp2KvjsjHRnXRHu_uQ_L7_Ox6dpEvfs4vZ6eLXCMrMUfKCsGNYthgxVFjoTRiUwJCBY3ilBkrtOaogNVWoK4pGoo1VEzYqjH8kHwZe9fBP2xt3MjeRW27Tq2s30ZZ8rIqac0TOB1BHXyMwTZyHVyvwiApyBeJMkmU_ySmwOdd8_amt-YV31lL83qcP7nODv9pk9-WV2-68zHr4sY-v2ZVuJdFerGQf37M5fn1d5gLfiWXia9G3iaVj84GGbWz6aOMC1ZvpPHuvTX-AndZn3g</recordid><startdate>198108</startdate><enddate>198108</enddate><creator>Ezzedeen, F.W.</creator><creator>Masoud, A.N.</creator><creator>Stohs, S.J.</creator><general>Elsevier Inc</general><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198108</creationdate><title>High-Performance Liquid Chromatographic Analysis of Iodochlorhydroxyquin in Plasma</title><author>Ezzedeen, F.W. ; Masoud, A.N. ; Stohs, S.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4274-412653da24f4834c46ac44f704080fa312de5cc34a029e54c914d1490825e8fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Anti-infectives-iodochlorhydroxyquin</topic><topic>Anti-infectives—iodochlorhydroxyquin, high-performance liquid chromatographic analysis, plasma</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Clioquinol - blood</topic><topic>high-performance liquid chromatographic analysis</topic><topic>High-performance liquid chromatography-analysis</topic><topic>High-performance liquid chromatography—analysis, iodochlorhydroxyquin, plasma</topic><topic>Humans</topic><topic>iodochlorhydroxyquin</topic><topic>Iodochlorhydroxyquin-high-performance liquid chromatographic analysis</topic><topic>Iodochlorhydroxyquin—high-performance liquid chromatographic analysis, plasma</topic><topic>plasma</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ezzedeen, F.W.</creatorcontrib><creatorcontrib>Masoud, A.N.</creatorcontrib><creatorcontrib>Stohs, S.J.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ezzedeen, F.W.</au><au>Masoud, A.N.</au><au>Stohs, S.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-Performance Liquid Chromatographic Analysis of Iodochlorhydroxyquin in Plasma</atitle><jtitle>Journal of pharmaceutical sciences</jtitle><addtitle>J. Pharm. Sci</addtitle><date>1981-08</date><risdate>1981</risdate><volume>70</volume><issue>8</issue><spage>889</spage><epage>891</epage><pages>889-891</pages><issn>0022-3549</issn><eissn>1520-6017</eissn><abstract>A simple isocratic high-performance liquid chromatographic procedure for the analysis of iodochlorhydroxyquin in human plasma is described. Protein was precipitated using perchloric acid, and the supernatant and precipitated protein fractions were extracted with ether. The ether phases were evaporated to dryness, reconstituted in mobile phase, and chromatographed. A reversed-phase microparticulate C18 column, a precolumn, and a UV detector at 256 nm were used. A mobile phase containing 80% methanol and 20% 0.05 M phosphoric acid was employed at a flow rate of 1 ml/min. Quantitation of iodochlorhydroxyquin in the 1-15-μg/ml range in human plasma was demonstrated with a coefficient of variance of 0.1-0.06. Hydrocortisone, which is used in combination with iodochlorhydroxyquin in ointments and creams, does not interfere in the assay.</abstract><cop>Washington</cop><pub>Elsevier Inc</pub><pmid>6458695</pmid><doi>10.1002/jps.2600700814</doi><tpages>3</tpages></addata></record> |
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| identifier | ISSN: 0022-3549 |
| ispartof | Journal of pharmaceutical sciences, 1981-08, Vol.70 (8), p.889-891 |
| issn | 0022-3549 1520-6017 |
| language | eng |
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| source | MEDLINE; Wiley Journals; Alma/SFX Local Collection |
| subjects | Anti-infectives-iodochlorhydroxyquin Anti-infectives—iodochlorhydroxyquin, high-performance liquid chromatographic analysis, plasma Chromatography, High Pressure Liquid Clioquinol - blood high-performance liquid chromatographic analysis High-performance liquid chromatography-analysis High-performance liquid chromatography—analysis, iodochlorhydroxyquin, plasma Humans iodochlorhydroxyquin Iodochlorhydroxyquin-high-performance liquid chromatographic analysis Iodochlorhydroxyquin—high-performance liquid chromatographic analysis, plasma plasma |
| title | High-Performance Liquid Chromatographic Analysis of Iodochlorhydroxyquin in Plasma |
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