Nucleotide binding and phosphorylation in microtubule assembly in vitro

Two non-hydrolyzable analogs of GTP, guanylyl-β,γ-methylene diphosphonate and guanylyl imidodiphosphate, have been found to induce rapid and efficient microtubule assembly in vitro by binding at the exchangeable site (E-site) on tubulin. Characterization of microtubule polymerization by several crit...

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Veröffentlicht in:	Journal of molecular biology 1977-10, Vol.115 (4), p.643-673
Hauptverfasser:	Penningroth, Stephen M., Kirschner, Marc W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Allosteric Site Glycoproteins - biosynthesis Guanosine Monophosphate - analogs & derivatives Guanosine Triphosphate - metabolism Guanylyl Imidodiphosphate - metabolism Hydrolysis Kinetics Magnesium Microscopy, Electron Microtubules - metabolism Microtubules - ultrastructure Nucleoside-Diphosphate Kinase - metabolism Tubulin - biosynthesis
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