The rapid concentration and purification of influenza virus from allantoic fluid
Influenza virus was quantitatively recovered from infectious allantoic fluid by precipitation with 8 percent (wt/vol) polyethylene glycol 6000 (PEG-6000). Virus concentrated by PEG-6000 treatment was rapidly purified by gel permeation chromatography through controlled pore glass beads. The purity of...
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| Veröffentlicht in: | Archives of virology 1977-01, Vol.55 (1-2), p.107-119 |
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| container_end_page | 119 |
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| container_issue | 1-2 |
| container_start_page | 107 |
| container_title | Archives of virology |
| container_volume | 55 |
| creator | Heyward, J T Klimas, R A Stapp, M D Obijeski, J F |
| description | Influenza virus was quantitatively recovered from infectious allantoic fluid by precipitation with 8 percent (wt/vol) polyethylene glycol 6000 (PEG-6000). Virus concentrated by PEG-6000 treatment was rapidly purified by gel permeation chromatography through controlled pore glass beads. The purity of virus preparations achieved by this method, when judged by three independent criteria, was comparable to virus preparations purified by conventional density gradient procedures. |
| doi_str_mv | 10.1007/BF01314484 |
| format | Article |
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Virus concentrated by PEG-6000 treatment was rapidly purified by gel permeation chromatography through controlled pore glass beads. The purity of virus preparations achieved by this method, when judged by three independent criteria, was comparable to virus preparations purified by conventional density gradient procedures.</description><subject>Allantois</subject><subject>Animals</subject><subject>Body Fluids - microbiology</subject><subject>Chemical Precipitation</subject><subject>Chick Embryo</subject><subject>Chromatography, Gel</subject><subject>Extraembryonic Membranes</subject><subject>Glycoproteins - analysis</subject><subject>Influenza A virus - isolation & purification</subject><subject>Influenza A virus - ultrastructure</subject><subject>Microbiological Techniques</subject><subject>Polyethylene Glycols</subject><subject>Viral Proteins - analysis</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM1LxDAQxYP4ta5ePHvIyYNQTTppmhx1cVVY0MN6LmmaYKRNatIK-tfbpaKnGd785vF4CJ1Tck0JKW_u1oQCZUywPbSgDPJMlFLsowUBwjLBiThGJym9EzIJUByhQwA-PS7Qy_bN4Kh612AdvDZ-iGpwwWPlG9yP0VmnZyFY7LxtR-O_Ff50cUzYxtBh1bbKD8FpPB1dc4oOrGqTOfudS_S6vt-uHrPN88PT6naT6VzQIZO2ZkJLTgsDmjTUQqmBK2skAV0KVdNSFiBoXkuppkVIxbkppBAKcmsAluhy9u1j-BhNGqrOJW12YUwYU1VCCYxzNoFXM6hjSCkaW_XRdSp-VZRUu_aq__Ym-OLXdaw70_yhc13wAxUOaXs</recordid><startdate>19770101</startdate><enddate>19770101</enddate><creator>Heyward, J T</creator><creator>Klimas, R A</creator><creator>Stapp, M D</creator><creator>Obijeski, J F</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19770101</creationdate><title>The rapid concentration and purification of influenza virus from allantoic fluid</title><author>Heyward, J T ; Klimas, R A ; Stapp, M D ; Obijeski, J F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c281t-9fb48c9615e3c0d1f37c36afe903c78ab17953812b99a53889a66e5988a32fe33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Allantois</topic><topic>Animals</topic><topic>Body Fluids - microbiology</topic><topic>Chemical Precipitation</topic><topic>Chick Embryo</topic><topic>Chromatography, Gel</topic><topic>Extraembryonic Membranes</topic><topic>Glycoproteins - analysis</topic><topic>Influenza A virus - isolation & purification</topic><topic>Influenza A virus - ultrastructure</topic><topic>Microbiological Techniques</topic><topic>Polyethylene Glycols</topic><topic>Viral Proteins - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heyward, J T</creatorcontrib><creatorcontrib>Klimas, R A</creatorcontrib><creatorcontrib>Stapp, M D</creatorcontrib><creatorcontrib>Obijeski, J F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heyward, J T</au><au>Klimas, R A</au><au>Stapp, M D</au><au>Obijeski, J F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The rapid concentration and purification of influenza virus from allantoic fluid</atitle><jtitle>Archives of virology</jtitle><addtitle>Arch Virol</addtitle><date>1977-01-01</date><risdate>1977</risdate><volume>55</volume><issue>1-2</issue><spage>107</spage><epage>119</epage><pages>107-119</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>Influenza virus was quantitatively recovered from infectious allantoic fluid by precipitation with 8 percent (wt/vol) polyethylene glycol 6000 (PEG-6000). Virus concentrated by PEG-6000 treatment was rapidly purified by gel permeation chromatography through controlled pore glass beads. The purity of virus preparations achieved by this method, when judged by three independent criteria, was comparable to virus preparations purified by conventional density gradient procedures.</abstract><cop>Austria</cop><pmid>336007</pmid><doi>10.1007/BF01314484</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-8608 |
| ispartof | Archives of virology, 1977-01, Vol.55 (1-2), p.107-119 |
| issn | 0304-8608 1432-8798 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73734664 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Allantois Animals Body Fluids - microbiology Chemical Precipitation Chick Embryo Chromatography, Gel Extraembryonic Membranes Glycoproteins - analysis Influenza A virus - isolation & purification Influenza A virus - ultrastructure Microbiological Techniques Polyethylene Glycols Viral Proteins - analysis |
| title | The rapid concentration and purification of influenza virus from allantoic fluid |
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