Epidermal growth factor-stimulated phosphorylation of tyrosine residues on a 120 000 dalton protein in mouse liver plasma membrane subfractions
The addition of Epidermal growth factor (EGF) to mouse liver sinusoidal plasma membrane subfractions, isolated from the blood face of hepatocytes, resulted in a two to threefold increase of 32P incorporation from [γ- 32P ]ATP into a 120 000 dalton protein component. The EGF-stimulation of the phosph...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 1981-09, Vol.102 (2), p.602-609 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 609 |
|---|---|
| container_issue | 2 |
| container_start_page | 602 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 102 |
| creator | Ehrhart, Jean-Claude Creuzet, Claudine Rollet, Emmanuelle Loeb, Jacques |
| description | The addition of Epidermal growth factor (EGF) to mouse liver sinusoidal plasma membrane subfractions, isolated from the blood face of hepatocytes, resulted in a two to threefold increase of
32P incorporation from [γ-
32P ]ATP into a 120 000 dalton protein component. The EGF-stimulation of the phosphorylation of this component was also evidenced in plasma membranes derived from the lateral and biliary faces of hepatocytes. A major phosphoamino acid residue after EGF stimulation was identified as tyrosine. An EGF-enhancement of the phosphorylation of threonine and serine residues also appears to be detectable. |
| doi_str_mv | 10.1016/S0006-291X(81)80174-X |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73732799</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X8180174X</els_id><sourcerecordid>73732799</sourcerecordid><originalsourceid>FETCH-LOGICAL-c275t-717cedeb698c21586b15540a39c184cb50df317bb6b12dce18785f2e528b30913</originalsourceid><addsrcrecordid>eNqFUU1r3DAQFaUl3ab9CQGdSntwo5Etyz6VEtIPCOTQBPYm9DFuVOy1K8kJ-yv6lzvJLrkWJITmzcx7M4-xMxCfQEB7_lMI0Vayh-2HDj52AnRTbV-wDYheVBJE85JtnlNeszc5_xYCoGn7E3bS9lq1Um3Y38slBkyTHfmvND-UOz5YX-ZU5RKndbQFA1_u5kw37ekb5x2fB172ac5xhzxhjmHFzCluOUjBiZMHOxYKLGkuGHeczjSvGfkY7zHxZbR5snzCySVLPfLqhkSs1Du_Za8GO2Z8d3xP2e3Xy5uL79XV9bcfF1-uKi-1KpUG7TGga_vOS1Bd60CpRti699A13ikRhhq0cwTI4BE63alBopKdq0UP9Sl7f-hLGv-Q_mKmmD2OIwkiqUbXupa67ylRHRI9TZwTDmZJcbJpb0CYRyPMkxHmccumA_NkhNlS3dmRYHUThueq4-YJ_3zAkaa8j5hM9hF3NFVM6IsJc_wPwz_cOJs1</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73732799</pqid></control><display><type>article</type><title>Epidermal growth factor-stimulated phosphorylation of tyrosine residues on a 120 000 dalton protein in mouse liver plasma membrane subfractions</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Ehrhart, Jean-Claude ; Creuzet, Claudine ; Rollet, Emmanuelle ; Loeb, Jacques</creator><creatorcontrib>Ehrhart, Jean-Claude ; Creuzet, Claudine ; Rollet, Emmanuelle ; Loeb, Jacques</creatorcontrib><description>The addition of Epidermal growth factor (EGF) to mouse liver sinusoidal plasma membrane subfractions, isolated from the blood face of hepatocytes, resulted in a two to threefold increase of
32P incorporation from [γ-
32P ]ATP into a 120 000 dalton protein component. The EGF-stimulation of the phosphorylation of this component was also evidenced in plasma membranes derived from the lateral and biliary faces of hepatocytes. A major phosphoamino acid residue after EGF stimulation was identified as tyrosine. An EGF-enhancement of the phosphorylation of threonine and serine residues also appears to be detectable.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/S0006-291X(81)80174-X</identifier><identifier>PMID: 6975625</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Membrane - metabolism ; Electrophoresis, Polyacrylamide Gel ; Epidermal Growth Factor - pharmacology ; Female ; Liver - metabolism ; Membrane Proteins - metabolism ; Mice ; Molecular Weight ; Phosphorylation ; Tyrosine</subject><ispartof>Biochemical and biophysical research communications, 1981-09, Vol.102 (2), p.602-609</ispartof><rights>1981 Academic Press, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c275t-717cedeb698c21586b15540a39c184cb50df317bb6b12dce18785f2e528b30913</citedby><cites>FETCH-LOGICAL-c275t-717cedeb698c21586b15540a39c184cb50df317bb6b12dce18785f2e528b30913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X8180174X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6975625$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ehrhart, Jean-Claude</creatorcontrib><creatorcontrib>Creuzet, Claudine</creatorcontrib><creatorcontrib>Rollet, Emmanuelle</creatorcontrib><creatorcontrib>Loeb, Jacques</creatorcontrib><title>Epidermal growth factor-stimulated phosphorylation of tyrosine residues on a 120 000 dalton protein in mouse liver plasma membrane subfractions</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The addition of Epidermal growth factor (EGF) to mouse liver sinusoidal plasma membrane subfractions, isolated from the blood face of hepatocytes, resulted in a two to threefold increase of
32P incorporation from [γ-
32P ]ATP into a 120 000 dalton protein component. The EGF-stimulation of the phosphorylation of this component was also evidenced in plasma membranes derived from the lateral and biliary faces of hepatocytes. A major phosphoamino acid residue after EGF stimulation was identified as tyrosine. An EGF-enhancement of the phosphorylation of threonine and serine residues also appears to be detectable.</description><subject>Animals</subject><subject>Cell Membrane - metabolism</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Female</subject><subject>Liver - metabolism</subject><subject>Membrane Proteins - metabolism</subject><subject>Mice</subject><subject>Molecular Weight</subject><subject>Phosphorylation</subject><subject>Tyrosine</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1r3DAQFaUl3ab9CQGdSntwo5Etyz6VEtIPCOTQBPYm9DFuVOy1K8kJ-yv6lzvJLrkWJITmzcx7M4-xMxCfQEB7_lMI0Vayh-2HDj52AnRTbV-wDYheVBJE85JtnlNeszc5_xYCoGn7E3bS9lq1Um3Y38slBkyTHfmvND-UOz5YX-ZU5RKndbQFA1_u5kw37ekb5x2fB172ac5xhzxhjmHFzCluOUjBiZMHOxYKLGkuGHeczjSvGfkY7zHxZbR5snzCySVLPfLqhkSs1Du_Za8GO2Z8d3xP2e3Xy5uL79XV9bcfF1-uKi-1KpUG7TGga_vOS1Bd60CpRti699A13ikRhhq0cwTI4BE63alBopKdq0UP9Sl7f-hLGv-Q_mKmmD2OIwkiqUbXupa67ylRHRI9TZwTDmZJcbJpb0CYRyPMkxHmccumA_NkhNlS3dmRYHUThueq4-YJ_3zAkaa8j5hM9hF3NFVM6IsJc_wPwz_cOJs1</recordid><startdate>19810930</startdate><enddate>19810930</enddate><creator>Ehrhart, Jean-Claude</creator><creator>Creuzet, Claudine</creator><creator>Rollet, Emmanuelle</creator><creator>Loeb, Jacques</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19810930</creationdate><title>Epidermal growth factor-stimulated phosphorylation of tyrosine residues on a 120 000 dalton protein in mouse liver plasma membrane subfractions</title><author>Ehrhart, Jean-Claude ; Creuzet, Claudine ; Rollet, Emmanuelle ; Loeb, Jacques</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c275t-717cedeb698c21586b15540a39c184cb50df317bb6b12dce18785f2e528b30913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Animals</topic><topic>Cell Membrane - metabolism</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>Female</topic><topic>Liver - metabolism</topic><topic>Membrane Proteins - metabolism</topic><topic>Mice</topic><topic>Molecular Weight</topic><topic>Phosphorylation</topic><topic>Tyrosine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ehrhart, Jean-Claude</creatorcontrib><creatorcontrib>Creuzet, Claudine</creatorcontrib><creatorcontrib>Rollet, Emmanuelle</creatorcontrib><creatorcontrib>Loeb, Jacques</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ehrhart, Jean-Claude</au><au>Creuzet, Claudine</au><au>Rollet, Emmanuelle</au><au>Loeb, Jacques</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Epidermal growth factor-stimulated phosphorylation of tyrosine residues on a 120 000 dalton protein in mouse liver plasma membrane subfractions</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1981-09-30</date><risdate>1981</risdate><volume>102</volume><issue>2</issue><spage>602</spage><epage>609</epage><pages>602-609</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The addition of Epidermal growth factor (EGF) to mouse liver sinusoidal plasma membrane subfractions, isolated from the blood face of hepatocytes, resulted in a two to threefold increase of
32P incorporation from [γ-
32P ]ATP into a 120 000 dalton protein component. The EGF-stimulation of the phosphorylation of this component was also evidenced in plasma membranes derived from the lateral and biliary faces of hepatocytes. A major phosphoamino acid residue after EGF stimulation was identified as tyrosine. An EGF-enhancement of the phosphorylation of threonine and serine residues also appears to be detectable.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6975625</pmid><doi>10.1016/S0006-291X(81)80174-X</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 1981-09, Vol.102 (2), p.602-609 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73732799 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Cell Membrane - metabolism Electrophoresis, Polyacrylamide Gel Epidermal Growth Factor - pharmacology Female Liver - metabolism Membrane Proteins - metabolism Mice Molecular Weight Phosphorylation Tyrosine |
| title | Epidermal growth factor-stimulated phosphorylation of tyrosine residues on a 120 000 dalton protein in mouse liver plasma membrane subfractions |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T00%3A48%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Epidermal%20growth%20factor-stimulated%20phosphorylation%20of%20tyrosine%20residues%20on%20a%20120%20000%20dalton%20protein%20in%20mouse%20liver%20plasma%20membrane%20subfractions&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Ehrhart,%20Jean-Claude&rft.date=1981-09-30&rft.volume=102&rft.issue=2&rft.spage=602&rft.epage=609&rft.pages=602-609&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/S0006-291X(81)80174-X&rft_dat=%3Cproquest_cross%3E73732799%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=73732799&rft_id=info:pmid/6975625&rft_els_id=S0006291X8180174X&rfr_iscdi=true |