Rapid changes in initiation-limited rates of protein synthesis in rat thymic lymphocytes correlate with energy charge
Depriving rat thymocytes of energy-providing substrates for 2 hr results in a 75–80% drop in rates of protein synthesis and a shift of ribosomes from active polysomes to inactive monomers and dimers. Glucose prevents these changes or, when added to starved cells, rapidly reverses them. Restoration o...
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| Veröffentlicht in: | Biochem. Biophys. Res. Commun.; (United States) 1977-11, Vol.79 (1), p.53-60 |
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| container_title | Biochem. Biophys. Res. Commun.; (United States) |
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| creator | Mendelsohn, Steven L. Nordeen, Steven K. Young, Donald A. |
| description | Depriving rat thymocytes of energy-providing substrates for 2 hr results in a 75–80% drop in rates of protein synthesis and a shift of ribosomes from active polysomes to inactive monomers and dimers. Glucose prevents these changes or, when added to starved cells, rapidly reverses them. Restoration of protein synthesis is associated with reversal of the 7% decline in the adenylate energy charge seen in starved cells. The data is consistent with the hypothesis that glucose increases initiation in starved cells, probably via effects on the balance of adenine nucleotides. Data with other substrates support this concept. The inability of glucose to fully restore energy charge in the presence of glucocorticoids or rotenone correlates with the limitation of protein synthesis. |
| doi_str_mv | 10.1016/0006-291X(77)90059-6 |
| format | Article |
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Glucose prevents these changes or, when added to starved cells, rapidly reverses them. Restoration of protein synthesis is associated with reversal of the 7% decline in the adenylate energy charge seen in starved cells. The data is consistent with the hypothesis that glucose increases initiation in starved cells, probably via effects on the balance of adenine nucleotides. Data with other substrates support this concept. 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Biophys. Res. Commun.; (United States)</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Depriving rat thymocytes of energy-providing substrates for 2 hr results in a 75–80% drop in rates of protein synthesis and a shift of ribosomes from active polysomes to inactive monomers and dimers. Glucose prevents these changes or, when added to starved cells, rapidly reverses them. Restoration of protein synthesis is associated with reversal of the 7% decline in the adenylate energy charge seen in starved cells. The data is consistent with the hypothesis that glucose increases initiation in starved cells, probably via effects on the balance of adenine nucleotides. Data with other substrates support this concept. The inability of glucose to fully restore energy charge in the presence of glucocorticoids or rotenone correlates with the limitation of protein synthesis.</description><subject>550200 - Biochemistry</subject><subject>Adenine Nucleotides - metabolism</subject><subject>ALDEHYDES</subject><subject>ANIMAL CELLS</subject><subject>ANIMALS</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BIOLOGICAL EFFECTS</subject><subject>BIOSYNTHESIS</subject><subject>CARBOHYDRATES</subject><subject>CELL CONSTITUENTS</subject><subject>Cell Fractionation</subject><subject>ENERGY BALANCE</subject><subject>Energy Metabolism</subject><subject>GLUCOSE</subject><subject>Glucose - metabolism</subject><subject>Glucose - pharmacology</subject><subject>HEXOSES</subject><subject>In Vitro Techniques</subject><subject>MAMMALS</subject><subject>METABOLISM</subject><subject>MONOSACCHARIDES</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANOIDS</subject><subject>Peptide Chain Initiation, Translational - drug effects</subject><subject>Polyribosomes - drug effects</subject><subject>Polyribosomes - metabolism</subject><subject>Polyribosomes - ultrastructure</subject><subject>Protein Biosynthesis</subject><subject>PROTEINS</subject><subject>RATS</subject><subject>RIBOSOMES</subject><subject>RODENTS</subject><subject>Rotenone - pharmacology</subject><subject>SACCHARIDES</subject><subject>SOMATIC CELLS</subject><subject>SYNTHESIS</subject><subject>T-Lymphocytes - metabolism</subject><subject>THYMOCYTES</subject><subject>VERTEBRATES</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kV-L1TAQxYO46t3Vb7APxQfRh-qkaZPmZUEW_ywsCKLgW8hNJ7eRNrkmuSv99qbbZR-FwDyc35nMnCHkksJ7CpR_AABeN5L-eivEOwnQyZo_ITsKEuqGQvuU7B6RF-Q8pd8AlLZcPifPGLCO9jty-q6PbqjMqP0BU-V8eS47nV3w9eRml3Goos5FC7Y6xpCxMGnxecTk7g1FrfK4zM5U0zIfx2CWFTchRpyKs_rr8lihx3hY1o_iAV-SM6unhK8e6gX5-fnTj-uv9e23LzfXH29rw3qWaw3UWiq6bt9JIYa2LMK5BdubPeutbhrZAVpmbctFIw1o2yLVjdAw9MB6wy7I661vSNmpZMo2ZjTBezRZ8Za1DJoCvdmgst2fE6asZpcMTpP2GE5JCSYawTtZwHYDTQwpRbTqGN2s46IoqPUiao1brXErIdT9RRQvtsuH_qf9jMOjaTtBka82GUsQdw7jOid6g4OL65hDcP_v_w8SS5xS</recordid><startdate>19771107</startdate><enddate>19771107</enddate><creator>Mendelsohn, Steven L.</creator><creator>Nordeen, Steven K.</creator><creator>Young, Donald A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19771107</creationdate><title>Rapid changes in initiation-limited rates of protein synthesis in rat thymic lymphocytes correlate with energy charge</title><author>Mendelsohn, Steven L. ; Nordeen, Steven K. ; Young, Donald A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-a01ff1755b5977d429166f0f8cb38fa22950ef3ff46729c0af4e1a27a0d8038c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>550200 - Biochemistry</topic><topic>Adenine Nucleotides - metabolism</topic><topic>ALDEHYDES</topic><topic>ANIMAL CELLS</topic><topic>ANIMALS</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BIOLOGICAL EFFECTS</topic><topic>BIOSYNTHESIS</topic><topic>CARBOHYDRATES</topic><topic>CELL CONSTITUENTS</topic><topic>Cell Fractionation</topic><topic>ENERGY BALANCE</topic><topic>Energy Metabolism</topic><topic>GLUCOSE</topic><topic>Glucose - metabolism</topic><topic>Glucose - pharmacology</topic><topic>HEXOSES</topic><topic>In Vitro Techniques</topic><topic>MAMMALS</topic><topic>METABOLISM</topic><topic>MONOSACCHARIDES</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANOIDS</topic><topic>Peptide Chain Initiation, Translational - drug effects</topic><topic>Polyribosomes - drug effects</topic><topic>Polyribosomes - metabolism</topic><topic>Polyribosomes - ultrastructure</topic><topic>Protein Biosynthesis</topic><topic>PROTEINS</topic><topic>RATS</topic><topic>RIBOSOMES</topic><topic>RODENTS</topic><topic>Rotenone - pharmacology</topic><topic>SACCHARIDES</topic><topic>SOMATIC CELLS</topic><topic>SYNTHESIS</topic><topic>T-Lymphocytes - metabolism</topic><topic>THYMOCYTES</topic><topic>VERTEBRATES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mendelsohn, Steven L.</creatorcontrib><creatorcontrib>Nordeen, Steven K.</creatorcontrib><creatorcontrib>Young, Donald A.</creatorcontrib><creatorcontrib>Univ. of Rochester, NY</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochem. 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Glucose prevents these changes or, when added to starved cells, rapidly reverses them. Restoration of protein synthesis is associated with reversal of the 7% decline in the adenylate energy charge seen in starved cells. The data is consistent with the hypothesis that glucose increases initiation in starved cells, probably via effects on the balance of adenine nucleotides. Data with other substrates support this concept. The inability of glucose to fully restore energy charge in the presence of glucocorticoids or rotenone correlates with the limitation of protein synthesis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>303518</pmid><doi>10.1016/0006-291X(77)90059-6</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | 550200 - Biochemistry Adenine Nucleotides - metabolism ALDEHYDES ANIMAL CELLS ANIMALS BASIC BIOLOGICAL SCIENCES BIOLOGICAL EFFECTS BIOSYNTHESIS CARBOHYDRATES CELL CONSTITUENTS Cell Fractionation ENERGY BALANCE Energy Metabolism GLUCOSE Glucose - metabolism Glucose - pharmacology HEXOSES In Vitro Techniques MAMMALS METABOLISM MONOSACCHARIDES ORGANIC COMPOUNDS ORGANOIDS Peptide Chain Initiation, Translational - drug effects Polyribosomes - drug effects Polyribosomes - metabolism Polyribosomes - ultrastructure Protein Biosynthesis PROTEINS RATS RIBOSOMES RODENTS Rotenone - pharmacology SACCHARIDES SOMATIC CELLS SYNTHESIS T-Lymphocytes - metabolism THYMOCYTES VERTEBRATES |
| title | Rapid changes in initiation-limited rates of protein synthesis in rat thymic lymphocytes correlate with energy charge |
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