Sources of error in neurotransmitter analysis
A compound having fluorescence characteristics similar to those of 5-hydroxytryptamine (5-HT), when reacted with ninhydrin, is present in several invertebrates ( Fasciola hepatica, Aplysia californica, Tritonia diomedia, and Hirudo medicinalis). However, this substance is not identical with 5-HT [ A...
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| Veröffentlicht in: | Analytical biochemistry 1977-11, Vol.83 (1), p.99-108 |
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| container_title | Analytical biochemistry |
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| creator | Tomosky-Sykes, T.K. Jardine, I. Mueller, J.F. Bueding, E. |
| description | A compound having fluorescence characteristics similar to those of 5-hydroxytryptamine (5-HT), when reacted with ninhydrin, is present in several invertebrates (
Fasciola hepatica, Aplysia californica, Tritonia diomedia, and
Hirudo medicinalis). However, this substance is not identical with 5-HT [
Andreini, G. C., Beretta, C., Faustini, R., and Gallina, G. (1970)
Experientia
26, 166–167]. We confirmed these findings for
Fasciola and also observed this substance in
Spirometra mansonoides and mouse brain. Using chromatography and amino acid analysis we identified this substance as lysine. While 5-HT is also demonstrable in
Spirometra, no 5-HT is present in
Fasciola. Although epinephrine and dopamine seem to be present in
Spirometra when the hydroxyindole technique of Laverty and Taylor [
Laverty, R., and Taylor, K. M. (1968)
Anal. Biochem.
22, 269–279] is used, neither amine is detectable in adult
Spirometra by mass spectrometric analysis. A relatively high concentration of tyrosine in
Spirometra could account for the apparent presence of dopamine. Therefore, lysine, tyrosine, and possibly other substances can be sources of error in the detection of biogenic amines in invertebrates. |
| doi_str_mv | 10.1016/0003-2697(77)90515-2 |
| format | Article |
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Fasciola hepatica, Aplysia californica, Tritonia diomedia, and
Hirudo medicinalis). However, this substance is not identical with 5-HT [
Andreini, G. C., Beretta, C., Faustini, R., and Gallina, G. (1970)
Experientia
26, 166–167]. We confirmed these findings for
Fasciola and also observed this substance in
Spirometra mansonoides and mouse brain. Using chromatography and amino acid analysis we identified this substance as lysine. While 5-HT is also demonstrable in
Spirometra, no 5-HT is present in
Fasciola. Although epinephrine and dopamine seem to be present in
Spirometra when the hydroxyindole technique of Laverty and Taylor [
Laverty, R., and Taylor, K. M. (1968)
Anal. Biochem.
22, 269–279] is used, neither amine is detectable in adult
Spirometra by mass spectrometric analysis. A relatively high concentration of tyrosine in
Spirometra could account for the apparent presence of dopamine. Therefore, lysine, tyrosine, and possibly other substances can be sources of error in the detection of biogenic amines in invertebrates.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(77)90515-2</identifier><identifier>PMID: 21601</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acids - analysis ; Animals ; Biogenic Amines - analysis ; Brain Chemistry ; Catecholamines - analysis ; Chromatography ; False Positive Reactions ; Fasciola - analysis ; Lysine - analysis ; Mass Spectrometry ; Mice ; Neurotransmitter Agents - analysis ; Ninhydrin ; Serotonin - analysis ; Sparganum - analysis ; Spectrometry, Fluorescence</subject><ispartof>Analytical biochemistry, 1977-11, Vol.83 (1), p.99-108</ispartof><rights>1977</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c355t-feadcb0d8d6c508ee019aa84a4df82256e74d9761f82121e4fa02af43cfc473a3</citedby><cites>FETCH-LOGICAL-c355t-feadcb0d8d6c508ee019aa84a4df82256e74d9761f82121e4fa02af43cfc473a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0003269777905152$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21601$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tomosky-Sykes, T.K.</creatorcontrib><creatorcontrib>Jardine, I.</creatorcontrib><creatorcontrib>Mueller, J.F.</creatorcontrib><creatorcontrib>Bueding, E.</creatorcontrib><title>Sources of error in neurotransmitter analysis</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A compound having fluorescence characteristics similar to those of 5-hydroxytryptamine (5-HT), when reacted with ninhydrin, is present in several invertebrates (
Fasciola hepatica, Aplysia californica, Tritonia diomedia, and
Hirudo medicinalis). However, this substance is not identical with 5-HT [
Andreini, G. C., Beretta, C., Faustini, R., and Gallina, G. (1970)
Experientia
26, 166–167]. We confirmed these findings for
Fasciola and also observed this substance in
Spirometra mansonoides and mouse brain. Using chromatography and amino acid analysis we identified this substance as lysine. While 5-HT is also demonstrable in
Spirometra, no 5-HT is present in
Fasciola. Although epinephrine and dopamine seem to be present in
Spirometra when the hydroxyindole technique of Laverty and Taylor [
Laverty, R., and Taylor, K. M. (1968)
Anal. Biochem.
22, 269–279] is used, neither amine is detectable in adult
Spirometra by mass spectrometric analysis. A relatively high concentration of tyrosine in
Spirometra could account for the apparent presence of dopamine. Therefore, lysine, tyrosine, and possibly other substances can be sources of error in the detection of biogenic amines in invertebrates.</description><subject>Amino Acids - analysis</subject><subject>Animals</subject><subject>Biogenic Amines - analysis</subject><subject>Brain Chemistry</subject><subject>Catecholamines - analysis</subject><subject>Chromatography</subject><subject>False Positive Reactions</subject><subject>Fasciola - analysis</subject><subject>Lysine - analysis</subject><subject>Mass Spectrometry</subject><subject>Mice</subject><subject>Neurotransmitter Agents - analysis</subject><subject>Ninhydrin</subject><subject>Serotonin - analysis</subject><subject>Sparganum - analysis</subject><subject>Spectrometry, Fluorescence</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LwzAYx4M4dE4_gR56Ej1Un6Rp0l4EGb7BwIN6DlnyBCJdM5NW2Le33caOnh7-_F_g-RFyReGOAhX3AFDkTNTyRsrbGkpa5uyITCnUIocC6mMyPUROyVlK3wCU8lKckAmjAuiU5B-hjwZTFlyGMYaY-TZrsY-hi7pNK991GDPd6maTfDonE6ebhBf7OyNfz0-f89d88f7yNn9c5KYoyy53qK1Zgq2sMCVUiEBrrSuuuXUVY6VAyW0tBR0UZRS508C044VxhstCFzNyvdtdx_DTY-rUyieDTaNbDH1SspC0ohUbgnwXNDGkFNGpdfQrHTeKghoZqRGAGgEoKdWWkRprl_v9frlCeyhtoQzuw87F4cVfj1El47E1aH1E0ykb_P_zf5iXdVY</recordid><startdate>197711</startdate><enddate>197711</enddate><creator>Tomosky-Sykes, T.K.</creator><creator>Jardine, I.</creator><creator>Mueller, J.F.</creator><creator>Bueding, E.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197711</creationdate><title>Sources of error in neurotransmitter analysis</title><author>Tomosky-Sykes, T.K. ; Jardine, I. ; Mueller, J.F. ; Bueding, E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c355t-feadcb0d8d6c508ee019aa84a4df82256e74d9761f82121e4fa02af43cfc473a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Amino Acids - analysis</topic><topic>Animals</topic><topic>Biogenic Amines - analysis</topic><topic>Brain Chemistry</topic><topic>Catecholamines - analysis</topic><topic>Chromatography</topic><topic>False Positive Reactions</topic><topic>Fasciola - analysis</topic><topic>Lysine - analysis</topic><topic>Mass Spectrometry</topic><topic>Mice</topic><topic>Neurotransmitter Agents - analysis</topic><topic>Ninhydrin</topic><topic>Serotonin - analysis</topic><topic>Sparganum - analysis</topic><topic>Spectrometry, Fluorescence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tomosky-Sykes, T.K.</creatorcontrib><creatorcontrib>Jardine, I.</creatorcontrib><creatorcontrib>Mueller, J.F.</creatorcontrib><creatorcontrib>Bueding, E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tomosky-Sykes, T.K.</au><au>Jardine, I.</au><au>Mueller, J.F.</au><au>Bueding, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sources of error in neurotransmitter analysis</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1977-11</date><risdate>1977</risdate><volume>83</volume><issue>1</issue><spage>99</spage><epage>108</epage><pages>99-108</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>A compound having fluorescence characteristics similar to those of 5-hydroxytryptamine (5-HT), when reacted with ninhydrin, is present in several invertebrates (
Fasciola hepatica, Aplysia californica, Tritonia diomedia, and
Hirudo medicinalis). However, this substance is not identical with 5-HT [
Andreini, G. C., Beretta, C., Faustini, R., and Gallina, G. (1970)
Experientia
26, 166–167]. We confirmed these findings for
Fasciola and also observed this substance in
Spirometra mansonoides and mouse brain. Using chromatography and amino acid analysis we identified this substance as lysine. While 5-HT is also demonstrable in
Spirometra, no 5-HT is present in
Fasciola. Although epinephrine and dopamine seem to be present in
Spirometra when the hydroxyindole technique of Laverty and Taylor [
Laverty, R., and Taylor, K. M. (1968)
Anal. Biochem.
22, 269–279] is used, neither amine is detectable in adult
Spirometra by mass spectrometric analysis. A relatively high concentration of tyrosine in
Spirometra could account for the apparent presence of dopamine. Therefore, lysine, tyrosine, and possibly other substances can be sources of error in the detection of biogenic amines in invertebrates.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>21601</pmid><doi>10.1016/0003-2697(77)90515-2</doi><tpages>10</tpages></addata></record> |
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| ispartof | Analytical biochemistry, 1977-11, Vol.83 (1), p.99-108 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acids - analysis Animals Biogenic Amines - analysis Brain Chemistry Catecholamines - analysis Chromatography False Positive Reactions Fasciola - analysis Lysine - analysis Mass Spectrometry Mice Neurotransmitter Agents - analysis Ninhydrin Serotonin - analysis Sparganum - analysis Spectrometry, Fluorescence |
| title | Sources of error in neurotransmitter analysis |
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