Accuracy and reproducibility of a quantitative electrophoresis of plasma lipoproteins: interlaboratory comparison and verification by ultracentrifugation
Fifty-three samples of sera from normal subjects and from patients with type IIa, IIb and IV hyperlipoproteinemia were shipped from Hannover to the collaborative laboratories in Vienna and Mannheim, which participated in the quantification of their lipoprotein fractions based on polyanion precipitat...
Gespeichert in:
| Veröffentlicht in: | Clinica chimica acta 1981-11, Vol.116 (3), p.381-387 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 387 |
|---|---|
| container_issue | 3 |
| container_start_page | 381 |
| container_title | Clinica chimica acta |
| container_volume | 116 |
| creator | Bojanovski, Dubo Wippermann, Burkhard Molinari, Ewald Schuster, Jürgen Canzler, Helmut |
| description | Fifty-three samples of sera from normal subjects and from patients with type IIa, IIb and IV hyperlipoproteinemia were shipped from Hannover to the collaborative laboratories in Vienna and Mannheim, which participated in the quantification of their lipoprotein fractions based on polyanion precipitation of electrophoretically separated lipoproteins followed by densitometric measurement of alpha-, prebeta- and beta-lipoproteins. Total serum cholesterol and cholesterol calculated from quantified lipoprotein fractions were highly correlated, the correlation coefficients ranged between
r = 0.93–0.98. Furthermore, we found a high concordance comparing the beta-, prebeta- and alpha-cholesterol with data obtained from lipoprotein fractionation and subsequent cholesterol measurement by ultracentrifugation. The obtained correlation coefficients were always higher than
r = 0.91.
High accuracy and reproducibility of the quantitative lipoprotein electrophoresis justify its recommendation for wide use in the field of clinical chemistry. |
| doi_str_mv | 10.1016/0009-8981(81)90057-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73710712</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0009898181900577</els_id><sourcerecordid>73710712</sourcerecordid><originalsourceid>FETCH-LOGICAL-c272t-a267f6b3fb6477fe244ea848231fcc691cf314d0137722fc53692c03a6c3bf5d3</originalsourceid><addsrcrecordid>eNp9UU1rFTEUDaLU16f_QCEr0cXYfL1kxoVQirWFQje6DpnMjUYyk2mSeTA_xX9r3gddCoGQc8899-YchN5R8pkSKq8IIV3Tdi392NJPHSE71agXaENbxRsuOvYSbZ4pr9Flzn_qUxBJL9CF7MSuY2KD_l5buyRjV2ymASeYUxwW63sffFlxdNjgp8VMxRdT_B4wBLAlxfl3TJB9PjDmYPJocPBzrN0F_JS_YD8VSMH0MZkS04ptHGeTfI7TcdAeknfeVs0K9CteQqlLwFQqvPw6wm_QK2dChrfne4t-3n77cXPXPDx-v7-5fmgsU6w0hknlZM9dL4VSDpgQYFrRMk6dtbKj1nEqBkK5Uow5u-OyY5ZwIy3v3W7gW_ThpFuXf1ogFz36bCEEM0FcslZcUaIoq0RxItoUc07g9Jz8aNKqKdGHRPTBbn2wW9dzTKR2b9H7s_7SjzA8N50jqPWvpzrUT-49JJ2th8nC4FP1Wg_R_3_APxhpoAE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73710712</pqid></control><display><type>article</type><title>Accuracy and reproducibility of a quantitative electrophoresis of plasma lipoproteins: interlaboratory comparison and verification by ultracentrifugation</title><source>MEDLINE</source><source>ScienceDirect Freedom Collection (Elsevier)</source><creator>Bojanovski, Dubo ; Wippermann, Burkhard ; Molinari, Ewald ; Schuster, Jürgen ; Canzler, Helmut</creator><creatorcontrib>Bojanovski, Dubo ; Wippermann, Burkhard ; Molinari, Ewald ; Schuster, Jürgen ; Canzler, Helmut</creatorcontrib><description>Fifty-three samples of sera from normal subjects and from patients with type IIa, IIb and IV hyperlipoproteinemia were shipped from Hannover to the collaborative laboratories in Vienna and Mannheim, which participated in the quantification of their lipoprotein fractions based on polyanion precipitation of electrophoretically separated lipoproteins followed by densitometric measurement of alpha-, prebeta- and beta-lipoproteins. Total serum cholesterol and cholesterol calculated from quantified lipoprotein fractions were highly correlated, the correlation coefficients ranged between
r = 0.93–0.98. Furthermore, we found a high concordance comparing the beta-, prebeta- and alpha-cholesterol with data obtained from lipoprotein fractionation and subsequent cholesterol measurement by ultracentrifugation. The obtained correlation coefficients were always higher than
r = 0.91.
High accuracy and reproducibility of the quantitative lipoprotein electrophoresis justify its recommendation for wide use in the field of clinical chemistry.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/0009-8981(81)90057-7</identifier><identifier>PMID: 6945924</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Cholesterol - blood ; Cholesterol, HDL ; Cholesterol, LDL ; Cholesterol, VLDL ; Humans ; Hyperlipoproteinemias - blood ; Lipoproteins - blood ; Lipoproteins, HDL - blood ; Lipoproteins, LDL - blood ; Lipoproteins, VLDL - blood ; Quality Control</subject><ispartof>Clinica chimica acta, 1981-11, Vol.116 (3), p.381-387</ispartof><rights>1981</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-a267f6b3fb6477fe244ea848231fcc691cf314d0137722fc53692c03a6c3bf5d3</citedby><cites>FETCH-LOGICAL-c272t-a267f6b3fb6477fe244ea848231fcc691cf314d0137722fc53692c03a6c3bf5d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0009-8981(81)90057-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6945924$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bojanovski, Dubo</creatorcontrib><creatorcontrib>Wippermann, Burkhard</creatorcontrib><creatorcontrib>Molinari, Ewald</creatorcontrib><creatorcontrib>Schuster, Jürgen</creatorcontrib><creatorcontrib>Canzler, Helmut</creatorcontrib><title>Accuracy and reproducibility of a quantitative electrophoresis of plasma lipoproteins: interlaboratory comparison and verification by ultracentrifugation</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>Fifty-three samples of sera from normal subjects and from patients with type IIa, IIb and IV hyperlipoproteinemia were shipped from Hannover to the collaborative laboratories in Vienna and Mannheim, which participated in the quantification of their lipoprotein fractions based on polyanion precipitation of electrophoretically separated lipoproteins followed by densitometric measurement of alpha-, prebeta- and beta-lipoproteins. Total serum cholesterol and cholesterol calculated from quantified lipoprotein fractions were highly correlated, the correlation coefficients ranged between
r = 0.93–0.98. Furthermore, we found a high concordance comparing the beta-, prebeta- and alpha-cholesterol with data obtained from lipoprotein fractionation and subsequent cholesterol measurement by ultracentrifugation. The obtained correlation coefficients were always higher than
r = 0.91.
High accuracy and reproducibility of the quantitative lipoprotein electrophoresis justify its recommendation for wide use in the field of clinical chemistry.</description><subject>Cholesterol - blood</subject><subject>Cholesterol, HDL</subject><subject>Cholesterol, LDL</subject><subject>Cholesterol, VLDL</subject><subject>Humans</subject><subject>Hyperlipoproteinemias - blood</subject><subject>Lipoproteins - blood</subject><subject>Lipoproteins, HDL - blood</subject><subject>Lipoproteins, LDL - blood</subject><subject>Lipoproteins, VLDL - blood</subject><subject>Quality Control</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1rFTEUDaLU16f_QCEr0cXYfL1kxoVQirWFQje6DpnMjUYyk2mSeTA_xX9r3gddCoGQc8899-YchN5R8pkSKq8IIV3Tdi392NJPHSE71agXaENbxRsuOvYSbZ4pr9Flzn_qUxBJL9CF7MSuY2KD_l5buyRjV2ymASeYUxwW63sffFlxdNjgp8VMxRdT_B4wBLAlxfl3TJB9PjDmYPJocPBzrN0F_JS_YD8VSMH0MZkS04ptHGeTfI7TcdAeknfeVs0K9CteQqlLwFQqvPw6wm_QK2dChrfne4t-3n77cXPXPDx-v7-5fmgsU6w0hknlZM9dL4VSDpgQYFrRMk6dtbKj1nEqBkK5Uow5u-OyY5ZwIy3v3W7gW_ThpFuXf1ogFz36bCEEM0FcslZcUaIoq0RxItoUc07g9Jz8aNKqKdGHRPTBbn2wW9dzTKR2b9H7s_7SjzA8N50jqPWvpzrUT-49JJ2th8nC4FP1Wg_R_3_APxhpoAE</recordid><startdate>19811111</startdate><enddate>19811111</enddate><creator>Bojanovski, Dubo</creator><creator>Wippermann, Burkhard</creator><creator>Molinari, Ewald</creator><creator>Schuster, Jürgen</creator><creator>Canzler, Helmut</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19811111</creationdate><title>Accuracy and reproducibility of a quantitative electrophoresis of plasma lipoproteins: interlaboratory comparison and verification by ultracentrifugation</title><author>Bojanovski, Dubo ; Wippermann, Burkhard ; Molinari, Ewald ; Schuster, Jürgen ; Canzler, Helmut</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-a267f6b3fb6477fe244ea848231fcc691cf314d0137722fc53692c03a6c3bf5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Cholesterol - blood</topic><topic>Cholesterol, HDL</topic><topic>Cholesterol, LDL</topic><topic>Cholesterol, VLDL</topic><topic>Humans</topic><topic>Hyperlipoproteinemias - blood</topic><topic>Lipoproteins - blood</topic><topic>Lipoproteins, HDL - blood</topic><topic>Lipoproteins, LDL - blood</topic><topic>Lipoproteins, VLDL - blood</topic><topic>Quality Control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bojanovski, Dubo</creatorcontrib><creatorcontrib>Wippermann, Burkhard</creatorcontrib><creatorcontrib>Molinari, Ewald</creatorcontrib><creatorcontrib>Schuster, Jürgen</creatorcontrib><creatorcontrib>Canzler, Helmut</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bojanovski, Dubo</au><au>Wippermann, Burkhard</au><au>Molinari, Ewald</au><au>Schuster, Jürgen</au><au>Canzler, Helmut</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Accuracy and reproducibility of a quantitative electrophoresis of plasma lipoproteins: interlaboratory comparison and verification by ultracentrifugation</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1981-11-11</date><risdate>1981</risdate><volume>116</volume><issue>3</issue><spage>381</spage><epage>387</epage><pages>381-387</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>Fifty-three samples of sera from normal subjects and from patients with type IIa, IIb and IV hyperlipoproteinemia were shipped from Hannover to the collaborative laboratories in Vienna and Mannheim, which participated in the quantification of their lipoprotein fractions based on polyanion precipitation of electrophoretically separated lipoproteins followed by densitometric measurement of alpha-, prebeta- and beta-lipoproteins. Total serum cholesterol and cholesterol calculated from quantified lipoprotein fractions were highly correlated, the correlation coefficients ranged between
r = 0.93–0.98. Furthermore, we found a high concordance comparing the beta-, prebeta- and alpha-cholesterol with data obtained from lipoprotein fractionation and subsequent cholesterol measurement by ultracentrifugation. The obtained correlation coefficients were always higher than
r = 0.91.
High accuracy and reproducibility of the quantitative lipoprotein electrophoresis justify its recommendation for wide use in the field of clinical chemistry.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>6945924</pmid><doi>10.1016/0009-8981(81)90057-7</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-8981 |
| ispartof | Clinica chimica acta, 1981-11, Vol.116 (3), p.381-387 |
| issn | 0009-8981 1873-3492 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73710712 |
| source | MEDLINE; ScienceDirect Freedom Collection (Elsevier) |
| subjects | Cholesterol - blood Cholesterol, HDL Cholesterol, LDL Cholesterol, VLDL Humans Hyperlipoproteinemias - blood Lipoproteins - blood Lipoproteins, HDL - blood Lipoproteins, LDL - blood Lipoproteins, VLDL - blood Quality Control |
| title | Accuracy and reproducibility of a quantitative electrophoresis of plasma lipoproteins: interlaboratory comparison and verification by ultracentrifugation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T15%3A13%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Accuracy%20and%20reproducibility%20of%20a%20quantitative%20electrophoresis%20of%20plasma%20lipoproteins:%20interlaboratory%20comparison%20and%20verification%20by%20ultracentrifugation&rft.jtitle=Clinica%20chimica%20acta&rft.au=Bojanovski,%20Dubo&rft.date=1981-11-11&rft.volume=116&rft.issue=3&rft.spage=381&rft.epage=387&rft.pages=381-387&rft.issn=0009-8981&rft.eissn=1873-3492&rft_id=info:doi/10.1016/0009-8981(81)90057-7&rft_dat=%3Cproquest_cross%3E73710712%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=73710712&rft_id=info:pmid/6945924&rft_els_id=0009898181900577&rfr_iscdi=true |