Restrictive distribution of asymmetric C-bands in the chromosome complement of the rhesus ( Macaca mulatto)
Lymphocyte chromosomes from a cercopithecoid species, Macaca mulatta, were studied for the occurrence of lateral asymmetry in constitutive heterochromatin. The technique consisted of growing the lymphocytes for one cell cycle in BrdUrd, staining with 33258 Hoechst, exposing them to UV light, treatin...
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| Veröffentlicht in: | Experimental cell research 1981-10, Vol.135 (2), p.424-427 |
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| creator | Ghosh, P.K. Nand, Reita Rani, Rajni |
| description | Lymphocyte chromosomes from a cercopithecoid species,
Macaca mulatta, were studied for the occurrence of lateral asymmetry in constitutive heterochromatin. The technique consisted of growing the lymphocytes for one cell cycle in BrdUrd, staining with 33258 Hoechst, exposing them to UV light, treating them with 2 SSC and staining with Giemsa. This procedure revealed asymmetric staining in the region of constitutive heterochromatin of the nucleolar organizer marker chromosome (no. 13 of the complement). In these chromosomes, the darkly staining region was confined at any given point to a single chromatid, while the corresponding region on the sister chromatid was lightly stained. This pattern of asymmetric staining in the constitutive heterochromatic region was not observed in any other chromosome of
Macaca mulatta. The lateral asymmetry of constitutive heterochromatin in this species is presumed to reflect the strand bias in the distribution of thymine in the alphoid DNA fractions. |
| doi_str_mv | 10.1016/0014-4827(81)90182-8 |
| format | Article |
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Macaca mulatta, were studied for the occurrence of lateral asymmetry in constitutive heterochromatin. The technique consisted of growing the lymphocytes for one cell cycle in BrdUrd, staining with 33258 Hoechst, exposing them to UV light, treating them with 2 SSC and staining with Giemsa. This procedure revealed asymmetric staining in the region of constitutive heterochromatin of the nucleolar organizer marker chromosome (no. 13 of the complement). In these chromosomes, the darkly staining region was confined at any given point to a single chromatid, while the corresponding region on the sister chromatid was lightly stained. This pattern of asymmetric staining in the constitutive heterochromatic region was not observed in any other chromosome of
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Macaca mulatta, were studied for the occurrence of lateral asymmetry in constitutive heterochromatin. The technique consisted of growing the lymphocytes for one cell cycle in BrdUrd, staining with 33258 Hoechst, exposing them to UV light, treating them with 2 SSC and staining with Giemsa. This procedure revealed asymmetric staining in the region of constitutive heterochromatin of the nucleolar organizer marker chromosome (no. 13 of the complement). In these chromosomes, the darkly staining region was confined at any given point to a single chromatid, while the corresponding region on the sister chromatid was lightly stained. This pattern of asymmetric staining in the constitutive heterochromatic region was not observed in any other chromosome of
Macaca mulatta. The lateral asymmetry of constitutive heterochromatin in this species is presumed to reflect the strand bias in the distribution of thymine in the alphoid DNA fractions.</description><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Chromatids - ultrastructure</subject><subject>Chromosome Banding</subject><subject>Chromosomes - ultrastructure</subject><subject>Functional Laterality</subject><subject>Heterochromatin - ultrastructure</subject><subject>Lymphocytes - ultrastructure</subject><subject>Macaca - genetics</subject><subject>Macaca mulatta - genetics</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE-LFDEQxYMo6-zqN1DISXYPrZV0ppO5CDLoKqwIoueQPxUm2umMSXphv73dzrBHqUMVvPeqqB8hrxi8ZcCGdwBMdEJxea3YzQ6Y4p16QjYMdtBxwflTsnm0PCeXtf4CAKXYcEEuZA-qB9iQ39-xthJdi_dIfVxnO7eYJ5oDNfUhJVxluu-smXylcaLtgNQdSk655rSMOR1HTDi1NbKK5YB1rvSafjVuKZrm0bSWb16QZ8GMFV-e-xX5-enjj_3n7u7b7Zf9h7vO9TC0rudbH3iQ6Jwy0gbkO8Atwy0qFNYPzDLOPVP94FFwaaUI1jsrkYlggvH9FXlz2nss-c-8_KdTrA7H0UyY56plL0HJnViM4mR0JddaMOhjicmUB81Ar4z1ClCvALVi-h9jrZbY6_P-2Sb0j6Ez1EV_f9JxefI-YtHVRZwc-ljQNe1z_P-BvxYsjVQ</recordid><startdate>198110</startdate><enddate>198110</enddate><creator>Ghosh, P.K.</creator><creator>Nand, Reita</creator><creator>Rani, Rajni</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198110</creationdate><title>Restrictive distribution of asymmetric C-bands in the chromosome complement of the rhesus ( Macaca mulatto)</title><author>Ghosh, P.K. ; Nand, Reita ; Rani, Rajni</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c306t-325df2f7ecc8a7bfe290e51e5e8e4bd61b122d1836de427b74fbdcb7e14fafad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Chromatids - ultrastructure</topic><topic>Chromosome Banding</topic><topic>Chromosomes - ultrastructure</topic><topic>Functional Laterality</topic><topic>Heterochromatin - ultrastructure</topic><topic>Lymphocytes - ultrastructure</topic><topic>Macaca - genetics</topic><topic>Macaca mulatta - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ghosh, P.K.</creatorcontrib><creatorcontrib>Nand, Reita</creatorcontrib><creatorcontrib>Rani, Rajni</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ghosh, P.K.</au><au>Nand, Reita</au><au>Rani, Rajni</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Restrictive distribution of asymmetric C-bands in the chromosome complement of the rhesus ( Macaca mulatto)</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1981-10</date><risdate>1981</risdate><volume>135</volume><issue>2</issue><spage>424</spage><epage>427</epage><pages>424-427</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>Lymphocyte chromosomes from a cercopithecoid species,
Macaca mulatta, were studied for the occurrence of lateral asymmetry in constitutive heterochromatin. The technique consisted of growing the lymphocytes for one cell cycle in BrdUrd, staining with 33258 Hoechst, exposing them to UV light, treating them with 2 SSC and staining with Giemsa. This procedure revealed asymmetric staining in the region of constitutive heterochromatin of the nucleolar organizer marker chromosome (no. 13 of the complement). In these chromosomes, the darkly staining region was confined at any given point to a single chromatid, while the corresponding region on the sister chromatid was lightly stained. This pattern of asymmetric staining in the constitutive heterochromatic region was not observed in any other chromosome of
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| ispartof | Experimental cell research, 1981-10, Vol.135 (2), p.424-427 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Cells, Cultured Chromatids - ultrastructure Chromosome Banding Chromosomes - ultrastructure Functional Laterality Heterochromatin - ultrastructure Lymphocytes - ultrastructure Macaca - genetics Macaca mulatta - genetics |
| title | Restrictive distribution of asymmetric C-bands in the chromosome complement of the rhesus ( Macaca mulatto) |
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