Compensatory increase in synaptosomal aldehyde reductase activity in rat brain after chronic barbital treatment
The changes in brain aldehyde reductase (AIR) activities during long-term treatment of rats with barbital were studied. NADH-linked AIR activity in the synaptosomal fraction increased rapidly, and supernatant AIR activity rose later during barbital treatment. With respect to NADPH-linked AIR, two di...
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| Veröffentlicht in: | Biochemical pharmacology 1981-10, Vol.30 (19), p.2657-2662 |
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| creator | Minegishi, Akemi Fukumori, Ryuji Satoh, Tetsuo Kitagawa, Haruo |
| description | The changes in brain aldehyde reductase (AIR) activities during long-term treatment of rats with barbital were studied. NADH-linked AIR activity in the synaptosomal fraction increased rapidly, and supernatant AIR activity rose later during barbital treatment. With respect to NADPH-linked AIR, two distinct
K
m
values were observed for
p-nitrobenzaldehyde. The low-
K
m
enzyme had a higher
K
i
value than the high-
K
m
enzyme. Although the elevation of NADPH-linked AIR activity under the routine assay conditions was less remarkable,
V
max values of the low-
K
m
enzyme were greatly increased in both the synaptosomal and the supernatant fractions by chronic barbital treatment. In addition, the
K
i
value of low-
K
m
AIR in synaptosomes was greater in barbital-treated animals than in control animals.
K
m
values were unchanged in either fraction by chronic barbital treatment. These data suggest that chronic barbital treatment resulted in a compensatory increase in the activity of AIR with low
K
m
, which is less sensitive to barbital and utilizes either NADH or NADPH, in synaptosomes. |
| doi_str_mv | 10.1016/0006-2952(81)90534-7 |
| format | Article |
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K
m
values were observed for
p-nitrobenzaldehyde. The low-
K
m
enzyme had a higher
K
i
value than the high-
K
m
enzyme. Although the elevation of NADPH-linked AIR activity under the routine assay conditions was less remarkable,
V
max values of the low-
K
m
enzyme were greatly increased in both the synaptosomal and the supernatant fractions by chronic barbital treatment. In addition, the
K
i
value of low-
K
m
AIR in synaptosomes was greater in barbital-treated animals than in control animals.
K
m
values were unchanged in either fraction by chronic barbital treatment. These data suggest that chronic barbital treatment resulted in a compensatory increase in the activity of AIR with low
K
m
, which is less sensitive to barbital and utilizes either NADH or NADPH, in synaptosomes.</description><identifier>ISSN: 0006-2952</identifier><identifier>EISSN: 1873-2968</identifier><identifier>DOI: 10.1016/0006-2952(81)90534-7</identifier><identifier>PMID: 7028043</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Alcohol Oxidoreductases - metabolism ; Animals ; Barbital - pharmacology ; Barbiturates - pharmacology ; Brain - enzymology ; In Vitro Techniques ; Kinetics ; Male ; NADP - metabolism ; Rats ; Rats, Inbred Strains ; Synaptosomes - enzymology ; Time Factors</subject><ispartof>Biochemical pharmacology, 1981-10, Vol.30 (19), p.2657-2662</ispartof><rights>1981</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-c1bfe5bbcb9292f26232ea5f7dd73ebb9176cd1eea95efa78a89e1d11498c9d93</citedby><cites>FETCH-LOGICAL-c388t-c1bfe5bbcb9292f26232ea5f7dd73ebb9176cd1eea95efa78a89e1d11498c9d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-2952(81)90534-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7028043$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Minegishi, Akemi</creatorcontrib><creatorcontrib>Fukumori, Ryuji</creatorcontrib><creatorcontrib>Satoh, Tetsuo</creatorcontrib><creatorcontrib>Kitagawa, Haruo</creatorcontrib><title>Compensatory increase in synaptosomal aldehyde reductase activity in rat brain after chronic barbital treatment</title><title>Biochemical pharmacology</title><addtitle>Biochem Pharmacol</addtitle><description>The changes in brain aldehyde reductase (AIR) activities during long-term treatment of rats with barbital were studied. NADH-linked AIR activity in the synaptosomal fraction increased rapidly, and supernatant AIR activity rose later during barbital treatment. With respect to NADPH-linked AIR, two distinct
K
m
values were observed for
p-nitrobenzaldehyde. The low-
K
m
enzyme had a higher
K
i
value than the high-
K
m
enzyme. Although the elevation of NADPH-linked AIR activity under the routine assay conditions was less remarkable,
V
max values of the low-
K
m
enzyme were greatly increased in both the synaptosomal and the supernatant fractions by chronic barbital treatment. In addition, the
K
i
value of low-
K
m
AIR in synaptosomes was greater in barbital-treated animals than in control animals.
K
m
values were unchanged in either fraction by chronic barbital treatment. These data suggest that chronic barbital treatment resulted in a compensatory increase in the activity of AIR with low
K
m
, which is less sensitive to barbital and utilizes either NADH or NADPH, in synaptosomes.</description><subject>Alcohol Oxidoreductases - metabolism</subject><subject>Animals</subject><subject>Barbital - pharmacology</subject><subject>Barbiturates - pharmacology</subject><subject>Brain - enzymology</subject><subject>In Vitro Techniques</subject><subject>Kinetics</subject><subject>Male</subject><subject>NADP - metabolism</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Synaptosomes - enzymology</subject><subject>Time Factors</subject><issn>0006-2952</issn><issn>1873-2968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1rGzEUFKUldd3-gxT2VNLDNvrwrqRLIJikLQR6Sc7iSXpLFHZXjiQH_O-jrU2O7el9zcyDGULOGf3BKOsvKaV9y3XHLxT7rmknNq18R1ZMSVHXvXpPVm-Qj-RTzk_LqHp2Rs4k5YpuxIrEbZx2OGcoMR2aMLuEkLE2TT7MsCsxxwnGBkaPjwePTUK_d2WBgCvhJZSF1CQojU1QOxgKpsY9pjgH11hINpTKL1W2TDiXz-TDAGPGL6e6Jg-3N_fbX-3dn5-_t9d3rRNKldYxO2BnrbOaaz7wnguO0A3SeynQWs1k7zxDBN3hAFKB0sg8YxutnPZarMm3o-4uxec95mKmkB2OI8wY99lIIakQuv8vkHWCcdqLCtwcgS7FnBMOZpfCBOlgGDVLIGax1yxuG8XM30DqmzX5etLf2wn9G-mUQL1fHe9Y3XgJmEx2AWeHPiR0xfgY_v3gFS8MnT0</recordid><startdate>19811001</startdate><enddate>19811001</enddate><creator>Minegishi, Akemi</creator><creator>Fukumori, Ryuji</creator><creator>Satoh, Tetsuo</creator><creator>Kitagawa, Haruo</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19811001</creationdate><title>Compensatory increase in synaptosomal aldehyde reductase activity in rat brain after chronic barbital treatment</title><author>Minegishi, Akemi ; Fukumori, Ryuji ; Satoh, Tetsuo ; Kitagawa, Haruo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-c1bfe5bbcb9292f26232ea5f7dd73ebb9176cd1eea95efa78a89e1d11498c9d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Alcohol Oxidoreductases - metabolism</topic><topic>Animals</topic><topic>Barbital - pharmacology</topic><topic>Barbiturates - pharmacology</topic><topic>Brain - enzymology</topic><topic>In Vitro Techniques</topic><topic>Kinetics</topic><topic>Male</topic><topic>NADP - metabolism</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Synaptosomes - enzymology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Minegishi, Akemi</creatorcontrib><creatorcontrib>Fukumori, Ryuji</creatorcontrib><creatorcontrib>Satoh, Tetsuo</creatorcontrib><creatorcontrib>Kitagawa, Haruo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Minegishi, Akemi</au><au>Fukumori, Ryuji</au><au>Satoh, Tetsuo</au><au>Kitagawa, Haruo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Compensatory increase in synaptosomal aldehyde reductase activity in rat brain after chronic barbital treatment</atitle><jtitle>Biochemical pharmacology</jtitle><addtitle>Biochem Pharmacol</addtitle><date>1981-10-01</date><risdate>1981</risdate><volume>30</volume><issue>19</issue><spage>2657</spage><epage>2662</epage><pages>2657-2662</pages><issn>0006-2952</issn><eissn>1873-2968</eissn><abstract>The changes in brain aldehyde reductase (AIR) activities during long-term treatment of rats with barbital were studied. NADH-linked AIR activity in the synaptosomal fraction increased rapidly, and supernatant AIR activity rose later during barbital treatment. With respect to NADPH-linked AIR, two distinct
K
m
values were observed for
p-nitrobenzaldehyde. The low-
K
m
enzyme had a higher
K
i
value than the high-
K
m
enzyme. Although the elevation of NADPH-linked AIR activity under the routine assay conditions was less remarkable,
V
max values of the low-
K
m
enzyme were greatly increased in both the synaptosomal and the supernatant fractions by chronic barbital treatment. In addition, the
K
i
value of low-
K
m
AIR in synaptosomes was greater in barbital-treated animals than in control animals.
K
m
values were unchanged in either fraction by chronic barbital treatment. These data suggest that chronic barbital treatment resulted in a compensatory increase in the activity of AIR with low
K
m
, which is less sensitive to barbital and utilizes either NADH or NADPH, in synaptosomes.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>7028043</pmid><doi>10.1016/0006-2952(81)90534-7</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical pharmacology, 1981-10, Vol.30 (19), p.2657-2662 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Alcohol Oxidoreductases - metabolism Animals Barbital - pharmacology Barbiturates - pharmacology Brain - enzymology In Vitro Techniques Kinetics Male NADP - metabolism Rats Rats, Inbred Strains Synaptosomes - enzymology Time Factors |
| title | Compensatory increase in synaptosomal aldehyde reductase activity in rat brain after chronic barbital treatment |
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