Nonspecific activation of murine spleen cells in vitro by a synthetic immunoadjuvant ( N-acetyl-muramyl- l-alanyl- d-isoglutamine)
We reported previously that synthetic N-acetyl-muramyl- l-alanyl- d-isoglutamine (MDP) displayed marked adjuvant activity but was devoid of mitogenicity in vitro. The data reported here establish that, under different cultural conditions, thymidine uptake and blast cells can be increased by MDP in s...
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| Veröffentlicht in: | Cellular immunology 1977-11, Vol.34 (1), p.49-56 |
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| container_title | Cellular immunology |
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| creator | Damais, Chantal Parant, Monique Chedid, Louis |
| description | We reported previously that synthetic
N-acetyl-muramyl-
l-alanyl-
d-isoglutamine (MDP) displayed marked adjuvant activity but was devoid of mitogenicity
in vitro. The data reported here establish that, under different cultural conditions, thymidine uptake and blast cells can be increased by MDP in spleen cells of DBA/2 and Balb/c mouse strains. Optimal responses were obtained on culture in a serum-free medium supplemented with 2-mercaptoethanol for 4 or 5 days. This effect was also obtained with spleen cells of Balb/c nude mice. When the synthetic MDP was compared to a natural water-soluble adjuvant (neo-WSA), extracted from
Mycobacterium smegmatis cells, both were found to stimulate [
3H] thymidine incorporation by mouse spleen cells. However, with the neo-WSA, the effect peaked on Day 2 and was weak or absent on Days 4 and 5. When the cells were cultured in a medium containing fetal calf serum, neo-WSA activation was completely abolished, while MDP-mediated stimulation was decreased. |
| doi_str_mv | 10.1016/0008-8749(77)90228-3 |
| format | Article |
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N-acetyl-muramyl-
l-alanyl-
d-isoglutamine (MDP) displayed marked adjuvant activity but was devoid of mitogenicity
in vitro. The data reported here establish that, under different cultural conditions, thymidine uptake and blast cells can be increased by MDP in spleen cells of DBA/2 and Balb/c mouse strains. Optimal responses were obtained on culture in a serum-free medium supplemented with 2-mercaptoethanol for 4 or 5 days. This effect was also obtained with spleen cells of Balb/c nude mice. When the synthetic MDP was compared to a natural water-soluble adjuvant (neo-WSA), extracted from
Mycobacterium smegmatis cells, both were found to stimulate [
3H] thymidine incorporation by mouse spleen cells. However, with the neo-WSA, the effect peaked on Day 2 and was weak or absent on Days 4 and 5. When the cells were cultured in a medium containing fetal calf serum, neo-WSA activation was completely abolished, while MDP-mediated stimulation was decreased.</description><identifier>ISSN: 0008-8749</identifier><identifier>EISSN: 1090-2163</identifier><identifier>DOI: 10.1016/0008-8749(77)90228-3</identifier><identifier>PMID: 562238</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Adjuvants, Immunologic - pharmacology ; Animals ; Cattle ; Cell Extracts - pharmacology ; Female ; Fetus - immunology ; Glycopeptides - pharmacology ; Lymphocyte Activation ; Mercaptoethanol - pharmacology ; Mice ; Mice, Inbred AKR ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mice, Inbred DBA ; Mice, Nude ; Mycobacterium - immunology ; Pregnancy ; Spleen - immunology ; Thymidine - metabolism ; Time Factors</subject><ispartof>Cellular immunology, 1977-11, Vol.34 (1), p.49-56</ispartof><rights>1977</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-63c5740ae5d40742a1d3982008449c2e3456f7e4079cecb36eebe2665b90a4993</citedby><cites>FETCH-LOGICAL-c337t-63c5740ae5d40742a1d3982008449c2e3456f7e4079cecb36eebe2665b90a4993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0008874977902283$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/562238$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Damais, Chantal</creatorcontrib><creatorcontrib>Parant, Monique</creatorcontrib><creatorcontrib>Chedid, Louis</creatorcontrib><title>Nonspecific activation of murine spleen cells in vitro by a synthetic immunoadjuvant ( N-acetyl-muramyl- l-alanyl- d-isoglutamine)</title><title>Cellular immunology</title><addtitle>Cell Immunol</addtitle><description>We reported previously that synthetic
N-acetyl-muramyl-
l-alanyl-
d-isoglutamine (MDP) displayed marked adjuvant activity but was devoid of mitogenicity
in vitro. The data reported here establish that, under different cultural conditions, thymidine uptake and blast cells can be increased by MDP in spleen cells of DBA/2 and Balb/c mouse strains. Optimal responses were obtained on culture in a serum-free medium supplemented with 2-mercaptoethanol for 4 or 5 days. This effect was also obtained with spleen cells of Balb/c nude mice. When the synthetic MDP was compared to a natural water-soluble adjuvant (neo-WSA), extracted from
Mycobacterium smegmatis cells, both were found to stimulate [
3H] thymidine incorporation by mouse spleen cells. However, with the neo-WSA, the effect peaked on Day 2 and was weak or absent on Days 4 and 5. When the cells were cultured in a medium containing fetal calf serum, neo-WSA activation was completely abolished, while MDP-mediated stimulation was decreased.</description><subject>Adjuvants, Immunologic - pharmacology</subject><subject>Animals</subject><subject>Cattle</subject><subject>Cell Extracts - pharmacology</subject><subject>Female</subject><subject>Fetus - immunology</subject><subject>Glycopeptides - pharmacology</subject><subject>Lymphocyte Activation</subject><subject>Mercaptoethanol - pharmacology</subject><subject>Mice</subject><subject>Mice, Inbred AKR</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred DBA</subject><subject>Mice, Nude</subject><subject>Mycobacterium - immunology</subject><subject>Pregnancy</subject><subject>Spleen - immunology</subject><subject>Thymidine - metabolism</subject><subject>Time Factors</subject><issn>0008-8749</issn><issn>1090-2163</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtvFSEYhonxdqz-gy5YmXaBchsYNiZNo9WkqRtdE4b5Rmlm4AjMSc7WXy7jabrs6iN5L-R9EDpn9AOjTH2klPak19JcaH1pKOc9Ec_QjlFDCWdKPEe7R8tr9KaUe0oZk4a-Qi87xbnod-jvXYplDz5MwWPnazi4GlLEacLLmkMEXPYzQMQe5rngEPEh1JzwcMQOl2Osv6G2ZFiWNSY33q8HFyu-wHfEeajHmbQWt7SLZ-JmF7fXSEJJv-a1uqV9cPkWvZjcXODdwz1DP798_nH9ldx-v_l2fXVLvBC6EiV8pyV10I2SaskdG4XpeRsopfEchOzUpKFpxoMfhAIYgCvVDYY6aYw4Q-9Pvfuc_qxQql1C2Wa5CGktVgtl-k52zShPRp9TKRkmu89hcfloGbUbebthtRtWq7X9T96KFjt_6F-HBcbH0Al1kz-dZGgbDwGyLT5A9DCGDL7aMYWn-_8Bc2iTdQ</recordid><startdate>197711</startdate><enddate>197711</enddate><creator>Damais, Chantal</creator><creator>Parant, Monique</creator><creator>Chedid, Louis</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197711</creationdate><title>Nonspecific activation of murine spleen cells in vitro by a synthetic immunoadjuvant ( N-acetyl-muramyl- l-alanyl- d-isoglutamine)</title><author>Damais, Chantal ; Parant, Monique ; Chedid, Louis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-63c5740ae5d40742a1d3982008449c2e3456f7e4079cecb36eebe2665b90a4993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Adjuvants, Immunologic - pharmacology</topic><topic>Animals</topic><topic>Cattle</topic><topic>Cell Extracts - pharmacology</topic><topic>Female</topic><topic>Fetus - immunology</topic><topic>Glycopeptides - pharmacology</topic><topic>Lymphocyte Activation</topic><topic>Mercaptoethanol - pharmacology</topic><topic>Mice</topic><topic>Mice, Inbred AKR</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred DBA</topic><topic>Mice, Nude</topic><topic>Mycobacterium - immunology</topic><topic>Pregnancy</topic><topic>Spleen - immunology</topic><topic>Thymidine - metabolism</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Damais, Chantal</creatorcontrib><creatorcontrib>Parant, Monique</creatorcontrib><creatorcontrib>Chedid, Louis</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Damais, Chantal</au><au>Parant, Monique</au><au>Chedid, Louis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nonspecific activation of murine spleen cells in vitro by a synthetic immunoadjuvant ( N-acetyl-muramyl- l-alanyl- d-isoglutamine)</atitle><jtitle>Cellular immunology</jtitle><addtitle>Cell Immunol</addtitle><date>1977-11</date><risdate>1977</risdate><volume>34</volume><issue>1</issue><spage>49</spage><epage>56</epage><pages>49-56</pages><issn>0008-8749</issn><eissn>1090-2163</eissn><abstract>We reported previously that synthetic
N-acetyl-muramyl-
l-alanyl-
d-isoglutamine (MDP) displayed marked adjuvant activity but was devoid of mitogenicity
in vitro. The data reported here establish that, under different cultural conditions, thymidine uptake and blast cells can be increased by MDP in spleen cells of DBA/2 and Balb/c mouse strains. Optimal responses were obtained on culture in a serum-free medium supplemented with 2-mercaptoethanol for 4 or 5 days. This effect was also obtained with spleen cells of Balb/c nude mice. When the synthetic MDP was compared to a natural water-soluble adjuvant (neo-WSA), extracted from
Mycobacterium smegmatis cells, both were found to stimulate [
3H] thymidine incorporation by mouse spleen cells. However, with the neo-WSA, the effect peaked on Day 2 and was weak or absent on Days 4 and 5. When the cells were cultured in a medium containing fetal calf serum, neo-WSA activation was completely abolished, while MDP-mediated stimulation was decreased.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>562238</pmid><doi>10.1016/0008-8749(77)90228-3</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Cellular immunology, 1977-11, Vol.34 (1), p.49-56 |
| issn | 0008-8749 1090-2163 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adjuvants, Immunologic - pharmacology Animals Cattle Cell Extracts - pharmacology Female Fetus - immunology Glycopeptides - pharmacology Lymphocyte Activation Mercaptoethanol - pharmacology Mice Mice, Inbred AKR Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred DBA Mice, Nude Mycobacterium - immunology Pregnancy Spleen - immunology Thymidine - metabolism Time Factors |
| title | Nonspecific activation of murine spleen cells in vitro by a synthetic immunoadjuvant ( N-acetyl-muramyl- l-alanyl- d-isoglutamine) |
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