Spectrophotometric titration of a single carboxyl group at the active site of ribonuclease T1

Low-pH-induced difference spectra for ribonuclease T1, which were determined using a reference solution at pH 6, consisted of a shorter wavelength component from 270 to 285 nm that relfected an ionization having a pKa of 3.54 and a longer wavelength component above 285 nm that reflected an ionizatio...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Biochemistry (Easton) 1977-10, Vol.16 (21), p.4568-4571
1. Verfasser:	Walz, Frederick G
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding Sites Glutamates Hydrogen-Ion Concentration Osmolar Concentration Ribonuclease T1 Ribonucleases Spectrophotometry, Ultraviolet Temperature
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!