An enzyme-linked immunoassay of testosterone
An enzyme-linked immunoassay of testosterone is described. The conjugation of testosterone with high specific activity horseradish peroxidase and a highly sensitive assay for this enzyme having previously been studied, here we describe the immobilization of the anti-testosterone antibody and the dev...
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| Veröffentlicht in: | Steroids 1977-07, Vol.30 (1), p.129-137 |
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| container_end_page | 137 |
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| container_issue | 1 |
| container_start_page | 129 |
| container_title | Steroids |
| container_volume | 30 |
| creator | Rajkowski, K.M. Cittanova, N. Urios, P. Jayle, M.F. |
| description | An enzyme-linked immunoassay of testosterone is described. The conjugation of testosterone with high specific activity horseradish peroxidase and a highly sensitive assay for this enzyme having previously been studied, here we describe the immobilization of the anti-testosterone antibody and the development of assay conditions permitting the determination of 50 pg to 1.5 ng testosterone in one working day. In this work attention has been paid to keeping the assay method as simple as possible. The method is discussed in terms of other enzyme-linked immunoassays for steroids. |
| doi_str_mv | 10.1016/0039-128X(77)90143-X |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0039-128X |
| ispartof | Steroids, 1977-07, Vol.30 (1), p.129-137 |
| issn | 0039-128X 1878-5867 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73690691 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Cross Reactions Enzyme-Linked Immunosorbent Assay Estradiol - immunology Gonadal Steroid Hormones - immunology Radioimmunoassay Testosterone - analysis Testosterone - immunology |
| title | An enzyme-linked immunoassay of testosterone |
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