Arachidonic acid 15-lipoxygenase from rabbit peritoneal polymorphonuclear leukocytes. Partial purification and properties

Arachidonic acid 15-lipoxygenase was purified from rabbit peritoneal polymorphonuclear leukocytes. The enzyme was recovered in the cytosol fraction after sonication and purified about 250-fold by acetone precipitation, column chromatography on CM52, Sephadex G-150, and hydroxyapatite. The enzyme cat...

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Veröffentlicht in:	The Journal of biological chemistry 1981-09, Vol.256 (18), p.9583-9592
Hauptverfasser:	Narumiya, S, Salmon, J A, Cottee, F H, Weatherley, B C, Flower, R J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Arachidonate Lipoxygenases Arachidonic Acid Arachidonic Acids - blood Cations, Divalent Chromatography, High Pressure Liquid Kinetics leukocytes lipoxygenase Lipoxygenase - blood Lipoxygenase - isolation & purification Mass Spectrometry Molecular Weight Neutrophils - enzymology purification Rabbits
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