method for simultaneous determination of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 in human plasma by using two steps of high-performance liquid chromatography
A method for simultaneous determination of 25-hy droxyvitamin D2 (25-OH-D2) and 25-hydroxyvitamin D3 (25-OH-D3) in human plasma has been developed by using two steps of high-performance liquid chromatography (HPLC). Lipids extracted from 0.5ml of human plasma were first subjected to the preparative...
Gespeichert in:
| Veröffentlicht in: | Journal of Nutritional Science and Vitaminology 1981, Vol.27(1), pp.43-54 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 54 |
|---|---|
| container_issue | 1 |
| container_start_page | 43 |
| container_title | Journal of Nutritional Science and Vitaminology |
| container_volume | 27 |
| creator | Okano, T Mizuno, N Shida, S Takahashi, N Kobayashi, T Kuroda, E Kodama, S Matsuo, T |
| description | A method for simultaneous determination of 25-hy droxyvitamin D2 (25-OH-D2) and 25-hydroxyvitamin D3 (25-OH-D3) in human plasma has been developed by using two steps of high-performance liquid chromatography (HPLC). Lipids extracted from 0.5ml of human plasma were first subjected to the preparative HPLC using a Nucleosil 5C18 column (reversed-phase type) and a 25-OH-D fraction containing 25-OH D2 and 25-OH-D3 was separated. The separated fraction was subsequently subjected to the analytical HPLC using a Zorbax SIL column (straight phase type). Since the peaks corresponding to 25-OH-D2 and 25-OH-D3 were clearly separated from one another on the chromatogram of the analytical HPLC, the metabolites could be simultaneously determined by estimating the respective peak heights. When the fractions corresponding to the respective peaks were separately collected by repeatedly applying rather large quantities of human plasma and were subjected to gas chromatography-mass spectrometry (GC-MS), they were identified as containing 25-OH-D2 and 25-OH-D3, respectively. The proposed method was applied to plasma samples of human adults taking 400 I.U./day of vitamin D2 for 8 weeks and the values were 22.5±8.1 ng/ml for 25-OH-D3 and 11.5±1.8ng/ml for 25-OH-D2 (mean±S. D.), respectively. |
| doi_str_mv | 10.3177/jnsv.27.43 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73683032</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73683032</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4653-3f740c895870a9b07308ee02e46e958d96b13aa721f2a33fdca109b228e1ca383</originalsourceid><addsrcrecordid>eNptks1u1DAURiMEKtPChj3CKxZIGWxfz9hZoil_UiUW0LV1kziJR0mc2k5LnocXxUNGs2JzLfl8Opbu5yx7w-gWmJQfj2N43HK5FfAs2zClWC6l4M-zDQXKcqEofZldh3CkVBRKqKvsal9IISXbZH8GEztXk8Z5Euww9xFH4-ZAahONH-yI0bqRuIbwXd4ttXe_l0cbMRFyywmO9f8AkDS7ecCRTD2GAUm5kDnYsSXxyZEQzRROzs62XT4Zn15P2cqQ3j7MtiZV592A0bUep255lb1osA_m9fm8ye6_fP51-Jbf_fj6_fDpLq_Efgc5NFLQShU7JSkWJZVAlTGUG7E36bIu9iUDRMlZwxGgqStktCg5V4ZVCApusverd_LuYTYh6sGGyvT9uhItYa-AAk_BD2uw8i4Ebxo9eTugXzSj-tSIPjWiudQCUvjt2TqXg6kv0XMFiR9WfgwRW3Ph6KOtevNPxQoJJx1bh4ALrTr02ozJ8m61NOg0tt4Gff-TU5Y-ALACFIO_zgerhQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73683032</pqid></control><display><type>article</type><title>method for simultaneous determination of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 in human plasma by using two steps of high-performance liquid chromatography</title><source>MEDLINE</source><source>J-STAGE日本語サイト (Free Access)</source><source>EZB Electronic Journals Library</source><creator>Okano, T ; Mizuno, N ; Shida, S ; Takahashi, N ; Kobayashi, T ; Kuroda, E ; Kodama, S ; Matsuo, T</creator><creatorcontrib>Okano, T ; Mizuno, N ; Shida, S ; Takahashi, N ; Kobayashi, T ; Kuroda, E ; Kodama, S ; Matsuo, T</creatorcontrib><description>A method for simultaneous determination of 25-hy droxyvitamin D2 (25-OH-D2) and 25-hydroxyvitamin D3 (25-OH-D3) in human plasma has been developed by using two steps of high-performance liquid chromatography (HPLC). Lipids extracted from 0.5ml of human plasma were first subjected to the preparative HPLC using a Nucleosil 5C18 column (reversed-phase type) and a 25-OH-D fraction containing 25-OH D2 and 25-OH-D3 was separated. The separated fraction was subsequently subjected to the analytical HPLC using a Zorbax SIL column (straight phase type). Since the peaks corresponding to 25-OH-D2 and 25-OH-D3 were clearly separated from one another on the chromatogram of the analytical HPLC, the metabolites could be simultaneously determined by estimating the respective peak heights. When the fractions corresponding to the respective peaks were separately collected by repeatedly applying rather large quantities of human plasma and were subjected to gas chromatography-mass spectrometry (GC-MS), they were identified as containing 25-OH-D2 and 25-OH-D3, respectively. The proposed method was applied to plasma samples of human adults taking 400 I.U./day of vitamin D2 for 8 weeks and the values were 22.5±8.1 ng/ml for 25-OH-D3 and 11.5±1.8ng/ml for 25-OH-D2 (mean±S. D.), respectively.</description><identifier>ISSN: 0301-4800</identifier><identifier>EISSN: 1881-7742</identifier><identifier>DOI: 10.3177/jnsv.27.43</identifier><identifier>PMID: 6974771</identifier><language>eng</language><publisher>Japan: Center for Academic Publications Japan</publisher><subject>25-hydroxycholecalciferol ; 25-hydroxyergocalciferol ; 25-Hydroxyvitamin D 2 ; 25-hydroxyvitamin D2 ; 25-hydroxyvitamin D3 ; Adult ; Calcifediol ; Chromatography, High Pressure Liquid - methods ; Ergocalciferols - administration & dosage ; Ergocalciferols - analogs & derivatives ; Ergocalciferols - blood ; Female ; Gas Chromatography-Mass Spectrometry ; high-performance liquid chromatography ; human nutrition ; human plasma ; Humans ; Hydroxycholecalciferols - blood ; Lipids - blood ; Male ; Middle Aged ; nutrition physiology ; ultraviolet detector ; vitamin D2 ; vitamin D3</subject><ispartof>Journal of Nutritional Science and Vitaminology, 1981, Vol.27(1), pp.43-54</ispartof><rights>the Center for Academic Publications Japan</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4653-3f740c895870a9b07308ee02e46e958d96b13aa721f2a33fdca109b228e1ca383</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6974771$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Okano, T</creatorcontrib><creatorcontrib>Mizuno, N</creatorcontrib><creatorcontrib>Shida, S</creatorcontrib><creatorcontrib>Takahashi, N</creatorcontrib><creatorcontrib>Kobayashi, T</creatorcontrib><creatorcontrib>Kuroda, E</creatorcontrib><creatorcontrib>Kodama, S</creatorcontrib><creatorcontrib>Matsuo, T</creatorcontrib><title>method for simultaneous determination of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 in human plasma by using two steps of high-performance liquid chromatography</title><title>Journal of Nutritional Science and Vitaminology</title><addtitle>J Nutr Sci Vitaminol</addtitle><description>A method for simultaneous determination of 25-hy droxyvitamin D2 (25-OH-D2) and 25-hydroxyvitamin D3 (25-OH-D3) in human plasma has been developed by using two steps of high-performance liquid chromatography (HPLC). Lipids extracted from 0.5ml of human plasma were first subjected to the preparative HPLC using a Nucleosil 5C18 column (reversed-phase type) and a 25-OH-D fraction containing 25-OH D2 and 25-OH-D3 was separated. The separated fraction was subsequently subjected to the analytical HPLC using a Zorbax SIL column (straight phase type). Since the peaks corresponding to 25-OH-D2 and 25-OH-D3 were clearly separated from one another on the chromatogram of the analytical HPLC, the metabolites could be simultaneously determined by estimating the respective peak heights. When the fractions corresponding to the respective peaks were separately collected by repeatedly applying rather large quantities of human plasma and were subjected to gas chromatography-mass spectrometry (GC-MS), they were identified as containing 25-OH-D2 and 25-OH-D3, respectively. The proposed method was applied to plasma samples of human adults taking 400 I.U./day of vitamin D2 for 8 weeks and the values were 22.5±8.1 ng/ml for 25-OH-D3 and 11.5±1.8ng/ml for 25-OH-D2 (mean±S. D.), respectively.</description><subject>25-hydroxycholecalciferol</subject><subject>25-hydroxyergocalciferol</subject><subject>25-Hydroxyvitamin D 2</subject><subject>25-hydroxyvitamin D2</subject><subject>25-hydroxyvitamin D3</subject><subject>Adult</subject><subject>Calcifediol</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Ergocalciferols - administration & dosage</subject><subject>Ergocalciferols - analogs & derivatives</subject><subject>Ergocalciferols - blood</subject><subject>Female</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>high-performance liquid chromatography</subject><subject>human nutrition</subject><subject>human plasma</subject><subject>Humans</subject><subject>Hydroxycholecalciferols - blood</subject><subject>Lipids - blood</subject><subject>Male</subject><subject>Middle Aged</subject><subject>nutrition physiology</subject><subject>ultraviolet detector</subject><subject>vitamin D2</subject><subject>vitamin D3</subject><issn>0301-4800</issn><issn>1881-7742</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptks1u1DAURiMEKtPChj3CKxZIGWxfz9hZoil_UiUW0LV1kziJR0mc2k5LnocXxUNGs2JzLfl8Opbu5yx7w-gWmJQfj2N43HK5FfAs2zClWC6l4M-zDQXKcqEofZldh3CkVBRKqKvsal9IISXbZH8GEztXk8Z5Euww9xFH4-ZAahONH-yI0bqRuIbwXd4ttXe_l0cbMRFyywmO9f8AkDS7ecCRTD2GAUm5kDnYsSXxyZEQzRROzs62XT4Zn15P2cqQ3j7MtiZV592A0bUep255lb1osA_m9fm8ye6_fP51-Jbf_fj6_fDpLq_Efgc5NFLQShU7JSkWJZVAlTGUG7E36bIu9iUDRMlZwxGgqStktCg5V4ZVCApusverd_LuYTYh6sGGyvT9uhItYa-AAk_BD2uw8i4Ebxo9eTugXzSj-tSIPjWiudQCUvjt2TqXg6kv0XMFiR9WfgwRW3Ph6KOtevNPxQoJJx1bh4ALrTr02ozJ8m61NOg0tt4Gff-TU5Y-ALACFIO_zgerhQ</recordid><startdate>1981</startdate><enddate>1981</enddate><creator>Okano, T</creator><creator>Mizuno, N</creator><creator>Shida, S</creator><creator>Takahashi, N</creator><creator>Kobayashi, T</creator><creator>Kuroda, E</creator><creator>Kodama, S</creator><creator>Matsuo, T</creator><general>Center for Academic Publications Japan</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1981</creationdate><title>method for simultaneous determination of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 in human plasma by using two steps of high-performance liquid chromatography</title><author>Okano, T ; Mizuno, N ; Shida, S ; Takahashi, N ; Kobayashi, T ; Kuroda, E ; Kodama, S ; Matsuo, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4653-3f740c895870a9b07308ee02e46e958d96b13aa721f2a33fdca109b228e1ca383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>25-hydroxycholecalciferol</topic><topic>25-hydroxyergocalciferol</topic><topic>25-Hydroxyvitamin D 2</topic><topic>25-hydroxyvitamin D2</topic><topic>25-hydroxyvitamin D3</topic><topic>Adult</topic><topic>Calcifediol</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Ergocalciferols - administration & dosage</topic><topic>Ergocalciferols - analogs & derivatives</topic><topic>Ergocalciferols - blood</topic><topic>Female</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>high-performance liquid chromatography</topic><topic>human nutrition</topic><topic>human plasma</topic><topic>Humans</topic><topic>Hydroxycholecalciferols - blood</topic><topic>Lipids - blood</topic><topic>Male</topic><topic>Middle Aged</topic><topic>nutrition physiology</topic><topic>ultraviolet detector</topic><topic>vitamin D2</topic><topic>vitamin D3</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Okano, T</creatorcontrib><creatorcontrib>Mizuno, N</creatorcontrib><creatorcontrib>Shida, S</creatorcontrib><creatorcontrib>Takahashi, N</creatorcontrib><creatorcontrib>Kobayashi, T</creatorcontrib><creatorcontrib>Kuroda, E</creatorcontrib><creatorcontrib>Kodama, S</creatorcontrib><creatorcontrib>Matsuo, T</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Nutritional Science and Vitaminology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Okano, T</au><au>Mizuno, N</au><au>Shida, S</au><au>Takahashi, N</au><au>Kobayashi, T</au><au>Kuroda, E</au><au>Kodama, S</au><au>Matsuo, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>method for simultaneous determination of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 in human plasma by using two steps of high-performance liquid chromatography</atitle><jtitle>Journal of Nutritional Science and Vitaminology</jtitle><addtitle>J Nutr Sci Vitaminol</addtitle><date>1981</date><risdate>1981</risdate><volume>27</volume><issue>1</issue><spage>43</spage><epage>54</epage><pages>43-54</pages><issn>0301-4800</issn><eissn>1881-7742</eissn><abstract>A method for simultaneous determination of 25-hy droxyvitamin D2 (25-OH-D2) and 25-hydroxyvitamin D3 (25-OH-D3) in human plasma has been developed by using two steps of high-performance liquid chromatography (HPLC). Lipids extracted from 0.5ml of human plasma were first subjected to the preparative HPLC using a Nucleosil 5C18 column (reversed-phase type) and a 25-OH-D fraction containing 25-OH D2 and 25-OH-D3 was separated. The separated fraction was subsequently subjected to the analytical HPLC using a Zorbax SIL column (straight phase type). Since the peaks corresponding to 25-OH-D2 and 25-OH-D3 were clearly separated from one another on the chromatogram of the analytical HPLC, the metabolites could be simultaneously determined by estimating the respective peak heights. When the fractions corresponding to the respective peaks were separately collected by repeatedly applying rather large quantities of human plasma and were subjected to gas chromatography-mass spectrometry (GC-MS), they were identified as containing 25-OH-D2 and 25-OH-D3, respectively. The proposed method was applied to plasma samples of human adults taking 400 I.U./day of vitamin D2 for 8 weeks and the values were 22.5±8.1 ng/ml for 25-OH-D3 and 11.5±1.8ng/ml for 25-OH-D2 (mean±S. D.), respectively.</abstract><cop>Japan</cop><pub>Center for Academic Publications Japan</pub><pmid>6974771</pmid><doi>10.3177/jnsv.27.43</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0301-4800 |
| ispartof | Journal of Nutritional Science and Vitaminology, 1981, Vol.27(1), pp.43-54 |
| issn | 0301-4800 1881-7742 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73683032 |
| source | MEDLINE; J-STAGE日本語サイト (Free Access); EZB Electronic Journals Library |
| subjects | 25-hydroxycholecalciferol 25-hydroxyergocalciferol 25-Hydroxyvitamin D 2 25-hydroxyvitamin D2 25-hydroxyvitamin D3 Adult Calcifediol Chromatography, High Pressure Liquid - methods Ergocalciferols - administration & dosage Ergocalciferols - analogs & derivatives Ergocalciferols - blood Female Gas Chromatography-Mass Spectrometry high-performance liquid chromatography human nutrition human plasma Humans Hydroxycholecalciferols - blood Lipids - blood Male Middle Aged nutrition physiology ultraviolet detector vitamin D2 vitamin D3 |
| title | method for simultaneous determination of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 in human plasma by using two steps of high-performance liquid chromatography |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T19%3A46%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=method%20for%20simultaneous%20determination%20of%2025-hydroxyvitamin%20D2%20and%2025-hydroxyvitamin%20D3%20in%20human%20plasma%20by%20using%20two%20steps%20of%20high-performance%20liquid%20chromatography&rft.jtitle=Journal%20of%20Nutritional%20Science%20and%20Vitaminology&rft.au=Okano,%20T&rft.date=1981&rft.volume=27&rft.issue=1&rft.spage=43&rft.epage=54&rft.pages=43-54&rft.issn=0301-4800&rft.eissn=1881-7742&rft_id=info:doi/10.3177/jnsv.27.43&rft_dat=%3Cproquest_cross%3E73683032%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=73683032&rft_id=info:pmid/6974771&rfr_iscdi=true |