Comparison of electrophoretic techniques for the analysis of human tear fluid proteins
Five electrophoretic techniques were tested for application to the analysis of human tear proteins: disc-, SDS- and gradient polyacrylamide gel electrophoresis, immunoelectrophoresis and isoelectric focusing. Protein patterns obtained from pooled normal tear fluid, tear fluid from keratoconjunctivit...
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| Veröffentlicht in: | Clinica chimica acta 1981-08, Vol.114 (2), p.207-218 |
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| container_title | Clinica chimica acta |
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| creator | Janssen, P.T. Van Bijsterveld, O.P. |
| description | Five electrophoretic techniques were tested for application to the analysis of human tear proteins: disc-, SDS- and gradient polyacrylamide gel electrophoresis, immunoelectrophoresis and isoelectric focusing. Protein patterns obtained from pooled normal tear fluid, tear fluid from keratoconjunctivitis sicca patients and from patients with chronic non-infectious conjunctivitis were compared. The four major components of human tear fluid were purified and their positions within the separation patterns were determined. The highest resolution and the clearest differences between the patterns of the three tear samples were obtained by SDS polyacrylamide gel electrophoresis. |
| doi_str_mv | 10.1016/0009-8981(81)90393-4 |
| format | Article |
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Protein patterns obtained from pooled normal tear fluid, tear fluid from keratoconjunctivitis sicca patients and from patients with chronic non-infectious conjunctivitis were compared. The four major components of human tear fluid were purified and their positions within the separation patterns were determined. 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Protein patterns obtained from pooled normal tear fluid, tear fluid from keratoconjunctivitis sicca patients and from patients with chronic non-infectious conjunctivitis were compared. The four major components of human tear fluid were purified and their positions within the separation patterns were determined. The highest resolution and the clearest differences between the patterns of the three tear samples were obtained by SDS polyacrylamide gel electrophoresis.</description><subject>Conjunctivitis - metabolism</subject><subject>Electrophoresis, Disc - methods</subject><subject>Electrophoresis, Polyacrylamide Gel - methods</subject><subject>Humans</subject><subject>Isoelectric Focusing</subject><subject>Keratoconjunctivitis - metabolism</subject><subject>Proteins - analysis</subject><subject>Proteins - isolation & purification</subject><subject>Sodium Dodecyl Sulfate</subject><subject>Tears - analysis</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLxDAQgIMo67r6DxR6Ej1U82iT9CLI4gsWvKjXkE2mNNI2a9IK--9N3cWjEAiZ-WYm8yF0TvANwYTfYoyrXFaSXElyXWFWsbw4QHMiBctZUdFDNP9DjtFJjJ_pWWBOZmgmqCxZwefoY-m7jQ4u-j7zdQYtmCH4TeMDDM5kA5imd18jxKz2IRsayHSv2210ccKbsdN9gnTI6nZ0NtsEP4Dr4yk6qnUb4Wx_L9D748Pb8jlfvT69LO9XuWGlGHJBObdWE6xLy9e4opymRQpCTamtNaWQxnJMpKG6pFZjrDkYUQtJuK1SjC3Q5a5vGjz9clCdiwbaVvfgx6gE44ITOYHFDjTBxxigVpvgOh22imA16VSTKzW5Uun86lRFKrvY9x_XHdi_or2_lL_b5SEt-e0gqGgc9AasC8mkst79P-AHn-mE4w</recordid><startdate>19810810</startdate><enddate>19810810</enddate><creator>Janssen, P.T.</creator><creator>Van Bijsterveld, O.P.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19810810</creationdate><title>Comparison of electrophoretic techniques for the analysis of human tear fluid proteins</title><author>Janssen, P.T. ; Van Bijsterveld, O.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-7266dda10a5d6b09262903412c5addc578cd6018c2a52da00a6ec7f7816d92a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Conjunctivitis - metabolism</topic><topic>Electrophoresis, Disc - methods</topic><topic>Electrophoresis, Polyacrylamide Gel - methods</topic><topic>Humans</topic><topic>Isoelectric Focusing</topic><topic>Keratoconjunctivitis - metabolism</topic><topic>Proteins - analysis</topic><topic>Proteins - isolation & purification</topic><topic>Sodium Dodecyl Sulfate</topic><topic>Tears - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Janssen, P.T.</creatorcontrib><creatorcontrib>Van Bijsterveld, O.P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Janssen, P.T.</au><au>Van Bijsterveld, O.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of electrophoretic techniques for the analysis of human tear fluid proteins</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1981-08-10</date><risdate>1981</risdate><volume>114</volume><issue>2</issue><spage>207</spage><epage>218</epage><pages>207-218</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>Five electrophoretic techniques were tested for application to the analysis of human tear proteins: disc-, SDS- and gradient polyacrylamide gel electrophoresis, immunoelectrophoresis and isoelectric focusing. Protein patterns obtained from pooled normal tear fluid, tear fluid from keratoconjunctivitis sicca patients and from patients with chronic non-infectious conjunctivitis were compared. The four major components of human tear fluid were purified and their positions within the separation patterns were determined. The highest resolution and the clearest differences between the patterns of the three tear samples were obtained by SDS polyacrylamide gel electrophoresis.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>7285346</pmid><doi>10.1016/0009-8981(81)90393-4</doi><tpages>12</tpages></addata></record> |
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| ispartof | Clinica chimica acta, 1981-08, Vol.114 (2), p.207-218 |
| issn | 0009-8981 1873-3492 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73676185 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Conjunctivitis - metabolism Electrophoresis, Disc - methods Electrophoresis, Polyacrylamide Gel - methods Humans Isoelectric Focusing Keratoconjunctivitis - metabolism Proteins - analysis Proteins - isolation & purification Sodium Dodecyl Sulfate Tears - analysis |
| title | Comparison of electrophoretic techniques for the analysis of human tear fluid proteins |
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