Prostacyclin (PGI 2) release accompanying angiotensin conversion in rat mesenteric vasculature
The relationship between angiotensin conversion and release of prostaglandins (PGs) was studies in isolated, perfused, mesenteric vasculature of rats. PGs in the mesenteric effluent were detected by bovine coronary artery and rat stomach strip, and by radioimmunoassay of 6-oxo-PGF 1α and PGE 2. Angi...
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Veröffentlicht in: | European journal of pharmacology 1981-03, Vol.70 (2), p.129-137 |
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creator | Dusting, Gregory J. Mullins, Elizabeth M. Nolan, Roger D. |
description | The relationship between angiotensin conversion and release of prostaglandins (PGs) was studies in isolated, perfused, mesenteric vasculature of rats. PGs in the mesenteric effluent were detected by bovine coronary artery and rat stomach strip, and by radioimmunoassay of 6-oxo-PGF
1α and PGE
2. Angiotensin II (AII) was assayed simultaneously using rabbit aorta. Both angiotensin I (AI) and AII released a prostacyclin (PGI
2)-like substance, but little PGE
2 from the mesentery, AII being 5 times more potent. Indomethacin (2.8 μM) abolished angiotensin-induced release of PGI
2. Of the AI (0.2–1.5 nmol) injected into the perfusion medium, 3–8% was converted to AII in passage through the mesentery. Captopril (4 μM), infused through the mesentery, inhibited AI-induced PGI
2 release, but did not affect release induced by AII. Release of the PGI
2-like substance by higher doses of AI (2–20 nmol) in the presence of captopril was always accompanied by concomitant contraction of rabbit aorta, indicating residual conversion of AI. Infusion of (Sar
1, Ala
8)AII (3–30 nM) through the mesentery abolished PGI
2 release by both peptides. By choosing doses of AI and AII which produced equivalent amounts of AII in the mesenteric effluent, it appeared that AII generated locally in the mesenteric vasculature was a more effective stimulus for PGI
2 generation than AII in the perfusion fluid. There is no evidence for intrinsic activity of AI, and release of the PGI
2-like substance appears to be mediated through an AII receptor. |
doi_str_mv | 10.1016/0014-2999(81)90207-7 |
format | Article |
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1α and PGE
2. Angiotensin II (AII) was assayed simultaneously using rabbit aorta. Both angiotensin I (AI) and AII released a prostacyclin (PGI
2)-like substance, but little PGE
2 from the mesentery, AII being 5 times more potent. Indomethacin (2.8 μM) abolished angiotensin-induced release of PGI
2. Of the AI (0.2–1.5 nmol) injected into the perfusion medium, 3–8% was converted to AII in passage through the mesentery. Captopril (4 μM), infused through the mesentery, inhibited AI-induced PGI
2 release, but did not affect release induced by AII. Release of the PGI
2-like substance by higher doses of AI (2–20 nmol) in the presence of captopril was always accompanied by concomitant contraction of rabbit aorta, indicating residual conversion of AI. Infusion of (Sar
1, Ala
8)AII (3–30 nM) through the mesentery abolished PGI
2 release by both peptides. By choosing doses of AI and AII which produced equivalent amounts of AII in the mesenteric effluent, it appeared that AII generated locally in the mesenteric vasculature was a more effective stimulus for PGI
2 generation than AII in the perfusion fluid. There is no evidence for intrinsic activity of AI, and release of the PGI
2-like substance appears to be mediated through an AII receptor.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/0014-2999(81)90207-7</identifier><identifier>PMID: 7021169</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>(Sar 1, Ala 8)angiotensin II ; Angiotensin ; Angiotensin I - metabolism ; Angiotensin II - biosynthesis ; Angiotensins - metabolism ; Angiotensins - pharmacology ; Animals ; Captopril ; Captopril - pharmacology ; Converting enzyme ; Epoprostenol - metabolism ; In Vitro Techniques ; Male ; Mesenteric Arteries - drug effects ; Mesenteric Arteries - metabolism ; Mesenteric vasculature ; Muscle, Smooth, Vascular - metabolism ; Prostacyclin (PGI 2) ; Prostaglandins - metabolism ; Rats ; Saralasin - pharmacology</subject><ispartof>European journal of pharmacology, 1981-03, Vol.70 (2), p.129-137</ispartof><rights>1981</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-af3363f89f5c2328220a1222ce7f993b640c10131154fcc52ec65650f1a67cef3</citedby><cites>FETCH-LOGICAL-c357t-af3363f89f5c2328220a1222ce7f993b640c10131154fcc52ec65650f1a67cef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-2999(81)90207-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7021169$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dusting, Gregory J.</creatorcontrib><creatorcontrib>Mullins, Elizabeth M.</creatorcontrib><creatorcontrib>Nolan, Roger D.</creatorcontrib><title>Prostacyclin (PGI 2) release accompanying angiotensin conversion in rat mesenteric vasculature</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>The relationship between angiotensin conversion and release of prostaglandins (PGs) was studies in isolated, perfused, mesenteric vasculature of rats. PGs in the mesenteric effluent were detected by bovine coronary artery and rat stomach strip, and by radioimmunoassay of 6-oxo-PGF
1α and PGE
2. Angiotensin II (AII) was assayed simultaneously using rabbit aorta. Both angiotensin I (AI) and AII released a prostacyclin (PGI
2)-like substance, but little PGE
2 from the mesentery, AII being 5 times more potent. Indomethacin (2.8 μM) abolished angiotensin-induced release of PGI
2. Of the AI (0.2–1.5 nmol) injected into the perfusion medium, 3–8% was converted to AII in passage through the mesentery. Captopril (4 μM), infused through the mesentery, inhibited AI-induced PGI
2 release, but did not affect release induced by AII. Release of the PGI
2-like substance by higher doses of AI (2–20 nmol) in the presence of captopril was always accompanied by concomitant contraction of rabbit aorta, indicating residual conversion of AI. Infusion of (Sar
1, Ala
8)AII (3–30 nM) through the mesentery abolished PGI
2 release by both peptides. By choosing doses of AI and AII which produced equivalent amounts of AII in the mesenteric effluent, it appeared that AII generated locally in the mesenteric vasculature was a more effective stimulus for PGI
2 generation than AII in the perfusion fluid. There is no evidence for intrinsic activity of AI, and release of the PGI
2-like substance appears to be mediated through an AII receptor.</description><subject>(Sar 1, Ala 8)angiotensin II</subject><subject>Angiotensin</subject><subject>Angiotensin I - metabolism</subject><subject>Angiotensin II - biosynthesis</subject><subject>Angiotensins - metabolism</subject><subject>Angiotensins - pharmacology</subject><subject>Animals</subject><subject>Captopril</subject><subject>Captopril - pharmacology</subject><subject>Converting enzyme</subject><subject>Epoprostenol - metabolism</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Mesenteric Arteries - drug effects</subject><subject>Mesenteric Arteries - metabolism</subject><subject>Mesenteric vasculature</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Prostacyclin (PGI 2)</subject><subject>Prostaglandins - metabolism</subject><subject>Rats</subject><subject>Saralasin - pharmacology</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LAzEQxYMotVa_gcKepD2s5k93s7kIUrQWCvagV0M6nZTIbrYmu4V-e7e29OhpGOa9N7wfIbeMPjDK8kdK2TjlSqlhwUaKcipTeUb6rJAqpZLxc9I_SS7JVYzflNJM8axHepJyxnLVJ1-LUMfGwA5K55PhYjpL-CgJWKKJmBiAutoYv3N-nRi_dnWDPnZCqP0WQ3S1T7otmCapMKJvMDhItiZCW5qmDXhNLqwpI94c54B8vr58TN7S-ft0NnmepyAy2aTGCpELWyibARe84JwaxjkHlFYpsczHFLrOgrFsbAEyjpBneUYtM7kEtGJA7g-5m1D_tBgbXbkIWJbGY91GLUUuOVNFJxwfhND1jgGt3gRXmbDTjOo9Vr1npvfMdMH0H9bOPSB3x_x2WeHqZDpy7O5Phzt2JbcOg47g0AOuXEBo9Kp2_z_4BVZahu8</recordid><startdate>19810312</startdate><enddate>19810312</enddate><creator>Dusting, Gregory J.</creator><creator>Mullins, Elizabeth M.</creator><creator>Nolan, Roger D.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19810312</creationdate><title>Prostacyclin (PGI 2) release accompanying angiotensin conversion in rat mesenteric vasculature</title><author>Dusting, Gregory J. ; Mullins, Elizabeth M. ; Nolan, Roger D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-af3363f89f5c2328220a1222ce7f993b640c10131154fcc52ec65650f1a67cef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>(Sar 1, Ala 8)angiotensin II</topic><topic>Angiotensin</topic><topic>Angiotensin I - metabolism</topic><topic>Angiotensin II - biosynthesis</topic><topic>Angiotensins - metabolism</topic><topic>Angiotensins - pharmacology</topic><topic>Animals</topic><topic>Captopril</topic><topic>Captopril - pharmacology</topic><topic>Converting enzyme</topic><topic>Epoprostenol - metabolism</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Mesenteric Arteries - drug effects</topic><topic>Mesenteric Arteries - metabolism</topic><topic>Mesenteric vasculature</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Prostacyclin (PGI 2)</topic><topic>Prostaglandins - metabolism</topic><topic>Rats</topic><topic>Saralasin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dusting, Gregory J.</creatorcontrib><creatorcontrib>Mullins, Elizabeth M.</creatorcontrib><creatorcontrib>Nolan, Roger D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dusting, Gregory J.</au><au>Mullins, Elizabeth M.</au><au>Nolan, Roger D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prostacyclin (PGI 2) release accompanying angiotensin conversion in rat mesenteric vasculature</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>1981-03-12</date><risdate>1981</risdate><volume>70</volume><issue>2</issue><spage>129</spage><epage>137</epage><pages>129-137</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><abstract>The relationship between angiotensin conversion and release of prostaglandins (PGs) was studies in isolated, perfused, mesenteric vasculature of rats. PGs in the mesenteric effluent were detected by bovine coronary artery and rat stomach strip, and by radioimmunoassay of 6-oxo-PGF
1α and PGE
2. Angiotensin II (AII) was assayed simultaneously using rabbit aorta. Both angiotensin I (AI) and AII released a prostacyclin (PGI
2)-like substance, but little PGE
2 from the mesentery, AII being 5 times more potent. Indomethacin (2.8 μM) abolished angiotensin-induced release of PGI
2. Of the AI (0.2–1.5 nmol) injected into the perfusion medium, 3–8% was converted to AII in passage through the mesentery. Captopril (4 μM), infused through the mesentery, inhibited AI-induced PGI
2 release, but did not affect release induced by AII. Release of the PGI
2-like substance by higher doses of AI (2–20 nmol) in the presence of captopril was always accompanied by concomitant contraction of rabbit aorta, indicating residual conversion of AI. Infusion of (Sar
1, Ala
8)AII (3–30 nM) through the mesentery abolished PGI
2 release by both peptides. By choosing doses of AI and AII which produced equivalent amounts of AII in the mesenteric effluent, it appeared that AII generated locally in the mesenteric vasculature was a more effective stimulus for PGI
2 generation than AII in the perfusion fluid. There is no evidence for intrinsic activity of AI, and release of the PGI
2-like substance appears to be mediated through an AII receptor.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>7021169</pmid><doi>10.1016/0014-2999(81)90207-7</doi><tpages>9</tpages></addata></record> |
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subjects | (Sar 1, Ala 8)angiotensin II Angiotensin Angiotensin I - metabolism Angiotensin II - biosynthesis Angiotensins - metabolism Angiotensins - pharmacology Animals Captopril Captopril - pharmacology Converting enzyme Epoprostenol - metabolism In Vitro Techniques Male Mesenteric Arteries - drug effects Mesenteric Arteries - metabolism Mesenteric vasculature Muscle, Smooth, Vascular - metabolism Prostacyclin (PGI 2) Prostaglandins - metabolism Rats Saralasin - pharmacology |
title | Prostacyclin (PGI 2) release accompanying angiotensin conversion in rat mesenteric vasculature |
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