Enhanced expression of the gene for beta-glycosidase of Thermus caldophilus GK24 and synthesis of galacto-oligosaccharides by the enzyme

The gene (bglT) encoding Tca beta-glycosidase (Thermus caldophilus GK24 beta-glycosidase) was overexpressed under the control of the trp promoter on a high-copy-number plasmid, pTRPES, in Escherichia coli W3110. The purified Tca beta-glycosidase enzyme was used in a galactosyl-transfer reaction to s...

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Veröffentlicht in:	Biotechnology and applied biochemistry 2003-10, Vol.38 (2), p.131-136
Hauptverfasser:	Choi, J.J, Oh, E.J, Lee, Y.J, Suh, D.S, Lee, J.H, Lee, S.W, Shin, H.T, Kwon, S.T
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals beta-glucosidase beta-Glucosidase - genetics beta-Glucosidase - isolation & purification beta-Glucosidase - metabolism Biological and medical sciences Biotechnology Enzyme Activation Fundamental and applied biological sciences. Psychology galactose Galactose - metabolism galactosyl-transfer activity gene expression genetic engineering Genetic Vectors Hydrogen-Ion Concentration oligosaccharides Oligosaccharides - biosynthesis Plasmids - genetics Temperature Thermus - enzymology Thermus - genetics Thermus caldophilus GK24 β-glycosidase Time Factors
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