A visible phagemid system for the estimation of Cre-mediated recombination efficiency

Diversity in phage antibody libraries is important for obtaining useful high-affinity antibodies. The Cre-lox system is generally employed to increase the size of phage antibody libraries. However, estimation of library sizes after Cre-mediated recombination is difficult, since time-consuming nucleo...

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Veröffentlicht in:	Journal of immunological methods 2003-09, Vol.280 (1), p.165-173
Hauptverfasser:	Kwon, Myung-Hee, Lee, Myung-Shin, Hong, Seung-Ho, Kim, Kyongmin Hwang, Shin, Ho-Joon, Park, Sun, Lee, Chi-Hyung, Kim, Hyung-Il
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Antibodies, immunoglobulins Antibody library size Base Sequence Biological and medical sciences Cre-mediated recombination DNA, Recombinant - genetics Fundamental and applied biological sciences. Psychology Fundamental immunology Genes, Immunoglobulin Genetic Vectors Immunoglobulin Heavy Chains - genetics Immunoglobulin Light Chains - genetics Immunoglobulin Variable Region - genetics Integrases - genetics Mice Molecular immunology Monoclonal antibodies Peptide Library Recombination efficiency Recombination, Genetic Viral Proteins - genetics Visible phagemid system X-gal staining
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