Inhibition of lipid peroxidation, protein glycation and elevation of membrane ion pump activity by taurine in RBC exposed to high glucose
Background: Supplementation of taurine, a sulfur containing amino acid has been found to be beneficial in counteracting oxidative stress and in preventing experimental diabetic neuropathy, nephropathy and retinopathy. Taurine has its own capacity to prevent the suppression of membrane-bound Na +/K +...
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description | Background: Supplementation of taurine, a sulfur containing amino acid has been found to be beneficial in counteracting oxidative stress and in preventing experimental diabetic neuropathy, nephropathy and retinopathy. Taurine has its own capacity to prevent the suppression of membrane-bound Na
+/K
+ATPase activity and prevent Ca
2+ overload. This study was undertaken to test whether taurine can reduce lipid peroxidation and glycosylation and can increase the Na
+/K
+- and Ca
2+-ATPase activities in high glucose-treated red blood cells (RBC).
Methods: Washed normal human RBC were incubated in phosphate-buffered saline with normal (6 mmol/l) or high glucose concentrations (45 mmol/l), with and without 50–150 μmol/l taurine in a shaking water bath at 37 °C for 24 h. Lipid peroxidation, glycated hemoglobin, glucose utilization and Na
+/K
+- and Ca
2+-ATPase activities were determined in the glucose-treated human RBC.
Results: Taurine significantly lowered the level of glycated hemoglobin (GHb) and lipid peroxidation in RBC exposed to high glucose concentrations. Stimulation of glucose utilization by RBC was significant in the presence of taurine both in normal and high glucose-treated RBC. The activities of Na
+/K
+- and Ca
2+-ATPases in RBC membranes were significantly lowered in high glucose-treated RBC. Taurine treatment significantly prevented the reduction in activities of Na
+/K
+- and Ca
2+-ATPases activities in high glucose-treated RBC.
Conclusions: The results show that taurine is important for the physiological functions of RBCs and the effects of taurine on glucose-treated RBC may have potential therapeutic relevance in diabetes. |
doi_str_mv | 10.1016/S0009-8981(03)00337-1 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73665717</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0009898103003371</els_id><sourcerecordid>73665717</sourcerecordid><originalsourceid>FETCH-LOGICAL-c509t-e4c7b25ebf575284a809f562b2ec15a2179dfe960b04195c11402b272f749be43</originalsourceid><addsrcrecordid>eNqFkdtu1DAQhi0EotvCI4B8AyoSATux4_gKwQpKpUqVOFxbtjPpGiVxajur7iPw1ji7W3rZK2tmvjn4_xF6RckHSmj98SchRBaNbOg5qd4RUlWioE_QijaiKiomy6do9R85Qacx_skhIzV9jk4o40vAV-jv5bhxxiXnR-w73LvJtXiC4O9cq5fsezwFn8CN-Kbf2X0K67HF0MNW37cNMJigR8BLPM3DhLVNbuvSDpsdTnoObimO-MeXNYa7yUdocfJ44242ee5sc-IFetbpPsLL43uGfn_7-mv9vbi6vrhcf74qLCcyFcCsMCUH03HBy4bphsiO16UpwVKuSypk24GsiSGMSm4pZSQXRdkJJg2w6gy9PczN_7qdISY1uGih7_P9fo5KVHXNBRUZ5AfQBh9jgE5NwQ067BQlavFA7T1Qi8CKVGrvgaK57_VxwWwGaB-6jqJn4M0R0NHqvsvKWRcfOJ7Jmi6XfjpwkOXYOggqWgejhdYFsEm13j1yyj8v3aST</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73665717</pqid></control><display><type>article</type><title>Inhibition of lipid peroxidation, protein glycation and elevation of membrane ion pump activity by taurine in RBC exposed to high glucose</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Nandhini, Thirugnanam Anitha ; Anuradha, Carani Venkataraman</creator><creatorcontrib>Nandhini, Thirugnanam Anitha ; Anuradha, Carani Venkataraman</creatorcontrib><description>Background: Supplementation of taurine, a sulfur containing amino acid has been found to be beneficial in counteracting oxidative stress and in preventing experimental diabetic neuropathy, nephropathy and retinopathy. Taurine has its own capacity to prevent the suppression of membrane-bound Na
+/K
+ATPase activity and prevent Ca
2+ overload. This study was undertaken to test whether taurine can reduce lipid peroxidation and glycosylation and can increase the Na
+/K
+- and Ca
2+-ATPase activities in high glucose-treated red blood cells (RBC).
Methods: Washed normal human RBC were incubated in phosphate-buffered saline with normal (6 mmol/l) or high glucose concentrations (45 mmol/l), with and without 50–150 μmol/l taurine in a shaking water bath at 37 °C for 24 h. Lipid peroxidation, glycated hemoglobin, glucose utilization and Na
+/K
+- and Ca
2+-ATPase activities were determined in the glucose-treated human RBC.
Results: Taurine significantly lowered the level of glycated hemoglobin (GHb) and lipid peroxidation in RBC exposed to high glucose concentrations. Stimulation of glucose utilization by RBC was significant in the presence of taurine both in normal and high glucose-treated RBC. The activities of Na
+/K
+- and Ca
2+-ATPases in RBC membranes were significantly lowered in high glucose-treated RBC. Taurine treatment significantly prevented the reduction in activities of Na
+/K
+- and Ca
2+-ATPases activities in high glucose-treated RBC.
Conclusions: The results show that taurine is important for the physiological functions of RBCs and the effects of taurine on glucose-treated RBC may have potential therapeutic relevance in diabetes.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/S0009-8981(03)00337-1</identifier><identifier>PMID: 14500045</identifier><identifier>CODEN: CCATAR</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Adult ; Associated diseases and complications ; Biological and medical sciences ; Blood Glucose - metabolism ; Blood Proteins - metabolism ; Ca 2+ATPase ; Calcium-Transporting ATPases - blood ; Diabetes. Impaired glucose tolerance ; Dose-Response Relationship, Drug ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Erythrocyte Membrane - metabolism ; Erythrocytes - drug effects ; Erythrocytes - metabolism ; Glucose - pharmacology ; Glycated hemoglobin ; Glycosylation ; Hemoglobins - metabolism ; Humans ; Hyperglycaemia ; Lipid peroxidation ; Lipid Peroxidation - drug effects ; Male ; Medical sciences ; Na +/K +ATPase ; Sodium-Potassium-Exchanging ATPase - blood ; Taurine ; Taurine - pharmacology</subject><ispartof>Clinica chimica acta, 2003-10, Vol.336 (1), p.129-135</ispartof><rights>2003 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c509t-e4c7b25ebf575284a809f562b2ec15a2179dfe960b04195c11402b272f749be43</citedby><cites>FETCH-LOGICAL-c509t-e4c7b25ebf575284a809f562b2ec15a2179dfe960b04195c11402b272f749be43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0009-8981(03)00337-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15145614$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14500045$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nandhini, Thirugnanam Anitha</creatorcontrib><creatorcontrib>Anuradha, Carani Venkataraman</creatorcontrib><title>Inhibition of lipid peroxidation, protein glycation and elevation of membrane ion pump activity by taurine in RBC exposed to high glucose</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>Background: Supplementation of taurine, a sulfur containing amino acid has been found to be beneficial in counteracting oxidative stress and in preventing experimental diabetic neuropathy, nephropathy and retinopathy. Taurine has its own capacity to prevent the suppression of membrane-bound Na
+/K
+ATPase activity and prevent Ca
2+ overload. This study was undertaken to test whether taurine can reduce lipid peroxidation and glycosylation and can increase the Na
+/K
+- and Ca
2+-ATPase activities in high glucose-treated red blood cells (RBC).
Methods: Washed normal human RBC were incubated in phosphate-buffered saline with normal (6 mmol/l) or high glucose concentrations (45 mmol/l), with and without 50–150 μmol/l taurine in a shaking water bath at 37 °C for 24 h. Lipid peroxidation, glycated hemoglobin, glucose utilization and Na
+/K
+- and Ca
2+-ATPase activities were determined in the glucose-treated human RBC.
Results: Taurine significantly lowered the level of glycated hemoglobin (GHb) and lipid peroxidation in RBC exposed to high glucose concentrations. Stimulation of glucose utilization by RBC was significant in the presence of taurine both in normal and high glucose-treated RBC. The activities of Na
+/K
+- and Ca
2+-ATPases in RBC membranes were significantly lowered in high glucose-treated RBC. Taurine treatment significantly prevented the reduction in activities of Na
+/K
+- and Ca
2+-ATPases activities in high glucose-treated RBC.
Conclusions: The results show that taurine is important for the physiological functions of RBCs and the effects of taurine on glucose-treated RBC may have potential therapeutic relevance in diabetes.</description><subject>Adult</subject><subject>Associated diseases and complications</subject><subject>Biological and medical sciences</subject><subject>Blood Glucose - metabolism</subject><subject>Blood Proteins - metabolism</subject><subject>Ca 2+ATPase</subject><subject>Calcium-Transporting ATPases - blood</subject><subject>Diabetes. Impaired glucose tolerance</subject><subject>Dose-Response Relationship, Drug</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>Erythrocyte Membrane - metabolism</subject><subject>Erythrocytes - drug effects</subject><subject>Erythrocytes - metabolism</subject><subject>Glucose - pharmacology</subject><subject>Glycated hemoglobin</subject><subject>Glycosylation</subject><subject>Hemoglobins - metabolism</subject><subject>Humans</subject><subject>Hyperglycaemia</subject><subject>Lipid peroxidation</subject><subject>Lipid Peroxidation - drug effects</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Na +/K +ATPase</subject><subject>Sodium-Potassium-Exchanging ATPase - blood</subject><subject>Taurine</subject><subject>Taurine - pharmacology</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkdtu1DAQhi0EotvCI4B8AyoSATux4_gKwQpKpUqVOFxbtjPpGiVxajur7iPw1ji7W3rZK2tmvjn4_xF6RckHSmj98SchRBaNbOg5qd4RUlWioE_QijaiKiomy6do9R85Qacx_skhIzV9jk4o40vAV-jv5bhxxiXnR-w73LvJtXiC4O9cq5fsezwFn8CN-Kbf2X0K67HF0MNW37cNMJigR8BLPM3DhLVNbuvSDpsdTnoObimO-MeXNYa7yUdocfJ44242ee5sc-IFetbpPsLL43uGfn_7-mv9vbi6vrhcf74qLCcyFcCsMCUH03HBy4bphsiO16UpwVKuSypk24GsiSGMSm4pZSQXRdkJJg2w6gy9PczN_7qdISY1uGih7_P9fo5KVHXNBRUZ5AfQBh9jgE5NwQ067BQlavFA7T1Qi8CKVGrvgaK57_VxwWwGaB-6jqJn4M0R0NHqvsvKWRcfOJ7Jmi6XfjpwkOXYOggqWgejhdYFsEm13j1yyj8v3aST</recordid><startdate>20031001</startdate><enddate>20031001</enddate><creator>Nandhini, Thirugnanam Anitha</creator><creator>Anuradha, Carani Venkataraman</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20031001</creationdate><title>Inhibition of lipid peroxidation, protein glycation and elevation of membrane ion pump activity by taurine in RBC exposed to high glucose</title><author>Nandhini, Thirugnanam Anitha ; Anuradha, Carani Venkataraman</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c509t-e4c7b25ebf575284a809f562b2ec15a2179dfe960b04195c11402b272f749be43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adult</topic><topic>Associated diseases and complications</topic><topic>Biological and medical sciences</topic><topic>Blood Glucose - metabolism</topic><topic>Blood Proteins - metabolism</topic><topic>Ca 2+ATPase</topic><topic>Calcium-Transporting ATPases - blood</topic><topic>Diabetes. Impaired glucose tolerance</topic><topic>Dose-Response Relationship, Drug</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>Erythrocyte Membrane - metabolism</topic><topic>Erythrocytes - drug effects</topic><topic>Erythrocytes - metabolism</topic><topic>Glucose - pharmacology</topic><topic>Glycated hemoglobin</topic><topic>Glycosylation</topic><topic>Hemoglobins - metabolism</topic><topic>Humans</topic><topic>Hyperglycaemia</topic><topic>Lipid peroxidation</topic><topic>Lipid Peroxidation - drug effects</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Na +/K +ATPase</topic><topic>Sodium-Potassium-Exchanging ATPase - blood</topic><topic>Taurine</topic><topic>Taurine - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nandhini, Thirugnanam Anitha</creatorcontrib><creatorcontrib>Anuradha, Carani Venkataraman</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nandhini, Thirugnanam Anitha</au><au>Anuradha, Carani Venkataraman</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of lipid peroxidation, protein glycation and elevation of membrane ion pump activity by taurine in RBC exposed to high glucose</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>2003-10-01</date><risdate>2003</risdate><volume>336</volume><issue>1</issue><spage>129</spage><epage>135</epage><pages>129-135</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><coden>CCATAR</coden><abstract>Background: Supplementation of taurine, a sulfur containing amino acid has been found to be beneficial in counteracting oxidative stress and in preventing experimental diabetic neuropathy, nephropathy and retinopathy. Taurine has its own capacity to prevent the suppression of membrane-bound Na
+/K
+ATPase activity and prevent Ca
2+ overload. This study was undertaken to test whether taurine can reduce lipid peroxidation and glycosylation and can increase the Na
+/K
+- and Ca
2+-ATPase activities in high glucose-treated red blood cells (RBC).
Methods: Washed normal human RBC were incubated in phosphate-buffered saline with normal (6 mmol/l) or high glucose concentrations (45 mmol/l), with and without 50–150 μmol/l taurine in a shaking water bath at 37 °C for 24 h. Lipid peroxidation, glycated hemoglobin, glucose utilization and Na
+/K
+- and Ca
2+-ATPase activities were determined in the glucose-treated human RBC.
Results: Taurine significantly lowered the level of glycated hemoglobin (GHb) and lipid peroxidation in RBC exposed to high glucose concentrations. Stimulation of glucose utilization by RBC was significant in the presence of taurine both in normal and high glucose-treated RBC. The activities of Na
+/K
+- and Ca
2+-ATPases in RBC membranes were significantly lowered in high glucose-treated RBC. Taurine treatment significantly prevented the reduction in activities of Na
+/K
+- and Ca
2+-ATPases activities in high glucose-treated RBC.
Conclusions: The results show that taurine is important for the physiological functions of RBCs and the effects of taurine on glucose-treated RBC may have potential therapeutic relevance in diabetes.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>14500045</pmid><doi>10.1016/S0009-8981(03)00337-1</doi><tpages>7</tpages></addata></record> |
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source | MEDLINE; Elsevier ScienceDirect Journals Complete |
subjects | Adult Associated diseases and complications Biological and medical sciences Blood Glucose - metabolism Blood Proteins - metabolism Ca 2+ATPase Calcium-Transporting ATPases - blood Diabetes. Impaired glucose tolerance Dose-Response Relationship, Drug Endocrine pancreas. Apud cells (diseases) Endocrinopathies Erythrocyte Membrane - metabolism Erythrocytes - drug effects Erythrocytes - metabolism Glucose - pharmacology Glycated hemoglobin Glycosylation Hemoglobins - metabolism Humans Hyperglycaemia Lipid peroxidation Lipid Peroxidation - drug effects Male Medical sciences Na +/K +ATPase Sodium-Potassium-Exchanging ATPase - blood Taurine Taurine - pharmacology |
title | Inhibition of lipid peroxidation, protein glycation and elevation of membrane ion pump activity by taurine in RBC exposed to high glucose |
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