Distribution of cyclic-GMP-[guanosin monophosphate]-dependent protein kinase in various rat tissues and cell lines determined by a sensitive and specific radioimmunoassay

Cyclic‐GMP‐dependent protein kinase purified from bovine lung was radioiodinated by the Bolton‐Hunter procedure yielding a specific radioactivity of 2200 Ci/mmol of enzyme. Using a specific precipitating rabbit antiserum to the cyclic‐GMP‐dependent protein kinase, a sensitive radioimmunoassay was de...

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Veröffentlicht in:	European journal of biochemistry 1981-08, Vol.118 (2), p.339-346
1. Verfasser:	Walter, U. (Wuerzburg Univ. (Germany, F.R.). Inst. fuer Physiologische Chemie)
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Brain - enzymology Cattle Cell Line Chromatography, DEAE-Cellulose Cyclic GMP - metabolism Cyclic IMP - metabolism Glioma - enzymology Hybrid Cells - enzymology Lung - enzymology Myocardium - enzymology Neuroblastoma - enzymology Protein Kinases - metabolism Rabbits Radioimmunoassay - methods Rats Tissue Distribution
Online-Zugang:	Volltext
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