Evidence for participation of sperm proteinases in fertilization of the solitary ascidian, Halocynthia roretzi: Effects of protease inhibitors
Effects of 15 proteinase inhibitors and an inhibitor against aminopeptidases on fertilization of the solitary ascidian, Halocynthia roretzi were studied in search of lysins. Fertilization of intact eggs was blocked by three trypsin inhibitors, leupeptin, antipain, and soybean trypsin inhibitor, and...
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| Veröffentlicht in: | Developmental biology 1981-08, Vol.86 (1), p.117-121 |
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| container_title | Developmental biology |
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| creator | Hoshi, Motonori Numakunai, Takaharu Sawada, Hitoshi |
| description | Effects of 15 proteinase inhibitors and an inhibitor against aminopeptidases on fertilization of the solitary ascidian,
Halocynthia roretzi were studied in search of lysins. Fertilization of intact eggs was blocked by three trypsin inhibitors, leupeptin, antipain, and soybean trypsin inhibitor, and by two chymotrypsin inhibitors, chymostatin and potato proteinase inhibitor I. On the other hand, the fertilization of naked eggs was not blocked at all by leupeptin and was only partially blocked by chymostatin at the concentrations sufficient for blocking that of intact eggs. This indicates that spermatozoa utilize trypsin-like and chymotrypsin-like proteinases probably as lysins for penetrating through the chorion. The chymotrypsin-like activity appears to be also required for some step besides sperm penetration through the egg investments. |
| doi_str_mv | 10.1016/0012-1606(81)90322-5 |
| format | Article |
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Halocynthia roretzi were studied in search of lysins. Fertilization of intact eggs was blocked by three trypsin inhibitors, leupeptin, antipain, and soybean trypsin inhibitor, and by two chymotrypsin inhibitors, chymostatin and potato proteinase inhibitor I. On the other hand, the fertilization of naked eggs was not blocked at all by leupeptin and was only partially blocked by chymostatin at the concentrations sufficient for blocking that of intact eggs. This indicates that spermatozoa utilize trypsin-like and chymotrypsin-like proteinases probably as lysins for penetrating through the chorion. The chymotrypsin-like activity appears to be also required for some step besides sperm penetration through the egg investments.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1016/0012-1606(81)90322-5</identifier><identifier>PMID: 7026318</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Endopeptidases - physiology ; Female ; Fertilization - drug effects ; Male ; Protease Inhibitors - pharmacology ; Sperm-Ovum Interactions ; Spermatozoa - enzymology ; Urochordata - physiology</subject><ispartof>Developmental biology, 1981-08, Vol.86 (1), p.117-121</ispartof><rights>1981</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-6991e661e1cf6c35d6b0882d47f7e057553e8890e396c40e8f4836152ad5db893</citedby><cites>FETCH-LOGICAL-c423t-6991e661e1cf6c35d6b0882d47f7e057553e8890e396c40e8f4836152ad5db893</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0012160681903225$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7026318$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hoshi, Motonori</creatorcontrib><creatorcontrib>Numakunai, Takaharu</creatorcontrib><creatorcontrib>Sawada, Hitoshi</creatorcontrib><title>Evidence for participation of sperm proteinases in fertilization of the solitary ascidian, Halocynthia roretzi: Effects of protease inhibitors</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>Effects of 15 proteinase inhibitors and an inhibitor against aminopeptidases on fertilization of the solitary ascidian,
Halocynthia roretzi were studied in search of lysins. Fertilization of intact eggs was blocked by three trypsin inhibitors, leupeptin, antipain, and soybean trypsin inhibitor, and by two chymotrypsin inhibitors, chymostatin and potato proteinase inhibitor I. On the other hand, the fertilization of naked eggs was not blocked at all by leupeptin and was only partially blocked by chymostatin at the concentrations sufficient for blocking that of intact eggs. This indicates that spermatozoa utilize trypsin-like and chymotrypsin-like proteinases probably as lysins for penetrating through the chorion. The chymotrypsin-like activity appears to be also required for some step besides sperm penetration through the egg investments.</description><subject>Animals</subject><subject>Endopeptidases - physiology</subject><subject>Female</subject><subject>Fertilization - drug effects</subject><subject>Male</subject><subject>Protease Inhibitors - pharmacology</subject><subject>Sperm-Ovum Interactions</subject><subject>Spermatozoa - enzymology</subject><subject>Urochordata - physiology</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kVFrFDEUhYNY6lr9Bwp5EgWnJpNJJuODIGVthUJfFHwL2eSGvTI7GZNsof0R_mYz3WUffcrDPee7uecQ8oazS864-sQYbxuumHqv-YeBibZt5DOy4myQjVTdr-dkdZK8IC9z_s0YE1qLc3Les1YJrlfk7_oePUwOaIiJzjYVdDjbgnGiMdA8Q9rROcUCONkMmeJEA1TViI8nVdkCzXHEYtMDtdmhRzt9pDd2jO5hKlu0NMUE5RE_03UI4EpebE_YCq3MLW6wxJRfkbNgxwyvj-8F-flt_ePqprm9u_5-9fW2cV0rSqOGgYNSHLgLygnp1YZp3fquDz0w2UspQOuBgRiU6xjo0GmhuGytl36jB3FB3h249Q9_9pCL2WF2MI52grjPphdKDh3vqrA7CF2KOScIZk64q3cazsxSg1kyNkvGRnPzVIOR1fb2yN9vduBPpmPudf7lMId65D1CMjW1pQaPqcZjfMT_L_gHTlOZVQ</recordid><startdate>198108</startdate><enddate>198108</enddate><creator>Hoshi, Motonori</creator><creator>Numakunai, Takaharu</creator><creator>Sawada, Hitoshi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198108</creationdate><title>Evidence for participation of sperm proteinases in fertilization of the solitary ascidian, Halocynthia roretzi: Effects of protease inhibitors</title><author>Hoshi, Motonori ; Numakunai, Takaharu ; Sawada, Hitoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-6991e661e1cf6c35d6b0882d47f7e057553e8890e396c40e8f4836152ad5db893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Animals</topic><topic>Endopeptidases - physiology</topic><topic>Female</topic><topic>Fertilization - drug effects</topic><topic>Male</topic><topic>Protease Inhibitors - pharmacology</topic><topic>Sperm-Ovum Interactions</topic><topic>Spermatozoa - enzymology</topic><topic>Urochordata - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hoshi, Motonori</creatorcontrib><creatorcontrib>Numakunai, Takaharu</creatorcontrib><creatorcontrib>Sawada, Hitoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hoshi, Motonori</au><au>Numakunai, Takaharu</au><au>Sawada, Hitoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence for participation of sperm proteinases in fertilization of the solitary ascidian, Halocynthia roretzi: Effects of protease inhibitors</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>1981-08</date><risdate>1981</risdate><volume>86</volume><issue>1</issue><spage>117</spage><epage>121</epage><pages>117-121</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><abstract>Effects of 15 proteinase inhibitors and an inhibitor against aminopeptidases on fertilization of the solitary ascidian,
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| fulltext | fulltext |
| identifier | ISSN: 0012-1606 |
| ispartof | Developmental biology, 1981-08, Vol.86 (1), p.117-121 |
| issn | 0012-1606 1095-564X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73659414 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Endopeptidases - physiology Female Fertilization - drug effects Male Protease Inhibitors - pharmacology Sperm-Ovum Interactions Spermatozoa - enzymology Urochordata - physiology |
| title | Evidence for participation of sperm proteinases in fertilization of the solitary ascidian, Halocynthia roretzi: Effects of protease inhibitors |
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