Effects of neonatal treatment with phytoestrogens, genistein and daidzein, on sex difference in female rat brain function: estrous cycle and lordosis

It is well known that neonatal exposure to estrogen induces masculinization or defeminization of the brain. In this study, the effects of neonatal treatment with two kinds of soybean isoflavone aglycone, genistein (GS) and daidzein (DZ), on the estrous cycle and lordosis behavior were investigated....

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Hormones and behavior 2003-08, Vol.44 (2), p.140-145, Article 140
Hauptverfasser: Kouki, Tom, Kishitake, Miki, Okamoto, Miho, Oosuka, Izumi, Takebe, Minoru, Yamanouchi, Korehito
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 145
container_issue 2
container_start_page 140
container_title Hormones and behavior
container_volume 44
creator Kouki, Tom
Kishitake, Miki
Okamoto, Miho
Oosuka, Izumi
Takebe, Minoru
Yamanouchi, Korehito
description It is well known that neonatal exposure to estrogen induces masculinization or defeminization of the brain. In this study, the effects of neonatal treatment with two kinds of soybean isoflavone aglycone, genistein (GS) and daidzein (DZ), on the estrous cycle and lordosis behavior were investigated. Female rats were injected subcutaneously with 1 mg GS, 1 mg DZ, 100 μg estradiol (E 2), or oil daily for 5 days from birth. As a result, vaginal opening was advanced in GS- or E 2-treated females. A vaginal smear check indicated that oil- or DZ-treated females showed a constant 4- or 5-day estrous cycle, whereas GS- or E 2-treated rats showed a persistent or prolonged estrus. Ovariectomy was performed in all females at 60 days of age. The ovaries in the GS- or E 2-treated groups were smaller than those in the oil- and DZ-treated groups and contained no corpora lutea. In the DZ group, although corpora lutea were seen, ovaries were smaller than that of control females. Behavioral tests were carried out after implantation of E 2-tubes. All of the oil- or DZ-treated females showed lordosis with a high lordosis quotient (LQ). On the other hand, as male rats, LQs were extremely low in the E 2-treated group, when compared to the oil-treated group. In the GS-treated group, the mean LQ was lower than that in the oil-treated group, but higher than those in the E 2-treated female or male groups. These results suggest that genistein acts as an estrogen in the sexual differentiation of the brain and causes defeminization of the brain in regulating lordosis and the estrous cycle in rats. In addition, neonatal daidzein also has some influence on ovarian function.
doi_str_mv 10.1016/S0018-506X(03)00122-3
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73657340</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0018506X03001223</els_id><sourcerecordid>19168291</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-c58fe571b449d69ae9f9c4db4e08316075d63a67f13759ea3597f067a10392053</originalsourceid><addsrcrecordid>eNqFkt9uFCEUxidGY9fqI2i40WjSURgGGPSiMU39kzTxQk28IywcLGYWtsDYru_h-8rsrm30pjeQQ37n8PF9NM1jgl8STPirzxiToWWYf3uO6YtadF1L7zQLgiVr-cDF3WZxjRw0D3L-UUvC-v5-c0Ao6WQ_8EXz-9Q5MCWj6FCAGHTRIyoJdFlBKOjSl3O0Pt-UCLmk-B1CPkJ19bmAD0gHi6z29lctjlAMKMMVsr6OTBAMoIo4WOkRUNIFLZOeD6Zgio_hNdqOnDIyG1OJedYYk43Z54fNPafHDI_2-2Hz9d3pl5MP7dmn9x9P3p61phd9aQ0bHDBBln0vLZcapJOmt8se8EAJx4JZTjUXjlDBJGjKpHCYC00wlR1m9LB5tpu7TvFiqnrUymcD46irF1NWgnImaI9vBYkkfOgkqSDbgSbFnBM4tU5-pdNGEazm4NQ2ODWnojBV2-AUrX1P9hdMyxXYm659UhV4ugd0Nnp0SQfj8w3HCOWdGCr35j8Bxhc9G16q_eO1jPxXxtW_Mo533VBd_-khqWz8HKX1qX4TZaO_5SF_AA_Iyeo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19168291</pqid></control><display><type>article</type><title>Effects of neonatal treatment with phytoestrogens, genistein and daidzein, on sex difference in female rat brain function: estrous cycle and lordosis</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Kouki, Tom ; Kishitake, Miki ; Okamoto, Miho ; Oosuka, Izumi ; Takebe, Minoru ; Yamanouchi, Korehito</creator><creatorcontrib>Kouki, Tom ; Kishitake, Miki ; Okamoto, Miho ; Oosuka, Izumi ; Takebe, Minoru ; Yamanouchi, Korehito</creatorcontrib><description>It is well known that neonatal exposure to estrogen induces masculinization or defeminization of the brain. In this study, the effects of neonatal treatment with two kinds of soybean isoflavone aglycone, genistein (GS) and daidzein (DZ), on the estrous cycle and lordosis behavior were investigated. Female rats were injected subcutaneously with 1 mg GS, 1 mg DZ, 100 μg estradiol (E 2), or oil daily for 5 days from birth. As a result, vaginal opening was advanced in GS- or E 2-treated females. A vaginal smear check indicated that oil- or DZ-treated females showed a constant 4- or 5-day estrous cycle, whereas GS- or E 2-treated rats showed a persistent or prolonged estrus. Ovariectomy was performed in all females at 60 days of age. The ovaries in the GS- or E 2-treated groups were smaller than those in the oil- and DZ-treated groups and contained no corpora lutea. In the DZ group, although corpora lutea were seen, ovaries were smaller than that of control females. Behavioral tests were carried out after implantation of E 2-tubes. All of the oil- or DZ-treated females showed lordosis with a high lordosis quotient (LQ). On the other hand, as male rats, LQs were extremely low in the E 2-treated group, when compared to the oil-treated group. In the GS-treated group, the mean LQ was lower than that in the oil-treated group, but higher than those in the E 2-treated female or male groups. These results suggest that genistein acts as an estrogen in the sexual differentiation of the brain and causes defeminization of the brain in regulating lordosis and the estrous cycle in rats. In addition, neonatal daidzein also has some influence on ovarian function.</description><identifier>ISSN: 0018-506X</identifier><identifier>EISSN: 1095-6867</identifier><identifier>DOI: 10.1016/S0018-506X(03)00122-3</identifier><identifier>PMID: 13129486</identifier><identifier>CODEN: HOBEAO</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Animals ; Animals, Newborn ; Behavioral psychophysiology ; Biological and medical sciences ; Brain ; daidzein ; Estradiol - physiology ; Estrogens, Non-Steroidal - pharmacology ; Estrous cycle ; Estrous Cycle - drug effects ; Female ; Fundamental and applied biological sciences. Psychology ; Genistein ; Genistein - pharmacology ; Hormones and behavior ; Isoflavones - pharmacology ; Lordosis ; Ovary - drug effects ; Phytoestrogen ; Phytoestrogens ; Plant Preparations ; Plants ; Posture - physiology ; Psychology. Psychoanalysis. Psychiatry ; Psychology. Psychophysiology ; Rat ; Rats ; Rats, Wistar ; Sex Characteristics ; Sex Differentiation - drug effects ; Sexual Behavior, Animal - drug effects ; Sexual differentiation</subject><ispartof>Hormones and behavior, 2003-08, Vol.44 (2), p.140-145, Article 140</ispartof><rights>2003 Elsevier Inc.</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-c58fe571b449d69ae9f9c4db4e08316075d63a67f13759ea3597f067a10392053</citedby><cites>FETCH-LOGICAL-c474t-c58fe571b449d69ae9f9c4db4e08316075d63a67f13759ea3597f067a10392053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0018506X03001223$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=15136278$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/13129486$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kouki, Tom</creatorcontrib><creatorcontrib>Kishitake, Miki</creatorcontrib><creatorcontrib>Okamoto, Miho</creatorcontrib><creatorcontrib>Oosuka, Izumi</creatorcontrib><creatorcontrib>Takebe, Minoru</creatorcontrib><creatorcontrib>Yamanouchi, Korehito</creatorcontrib><title>Effects of neonatal treatment with phytoestrogens, genistein and daidzein, on sex difference in female rat brain function: estrous cycle and lordosis</title><title>Hormones and behavior</title><addtitle>Horm Behav</addtitle><description>It is well known that neonatal exposure to estrogen induces masculinization or defeminization of the brain. In this study, the effects of neonatal treatment with two kinds of soybean isoflavone aglycone, genistein (GS) and daidzein (DZ), on the estrous cycle and lordosis behavior were investigated. Female rats were injected subcutaneously with 1 mg GS, 1 mg DZ, 100 μg estradiol (E 2), or oil daily for 5 days from birth. As a result, vaginal opening was advanced in GS- or E 2-treated females. A vaginal smear check indicated that oil- or DZ-treated females showed a constant 4- or 5-day estrous cycle, whereas GS- or E 2-treated rats showed a persistent or prolonged estrus. Ovariectomy was performed in all females at 60 days of age. The ovaries in the GS- or E 2-treated groups were smaller than those in the oil- and DZ-treated groups and contained no corpora lutea. In the DZ group, although corpora lutea were seen, ovaries were smaller than that of control females. Behavioral tests were carried out after implantation of E 2-tubes. All of the oil- or DZ-treated females showed lordosis with a high lordosis quotient (LQ). On the other hand, as male rats, LQs were extremely low in the E 2-treated group, when compared to the oil-treated group. In the GS-treated group, the mean LQ was lower than that in the oil-treated group, but higher than those in the E 2-treated female or male groups. These results suggest that genistein acts as an estrogen in the sexual differentiation of the brain and causes defeminization of the brain in regulating lordosis and the estrous cycle in rats. In addition, neonatal daidzein also has some influence on ovarian function.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Behavioral psychophysiology</subject><subject>Biological and medical sciences</subject><subject>Brain</subject><subject>daidzein</subject><subject>Estradiol - physiology</subject><subject>Estrogens, Non-Steroidal - pharmacology</subject><subject>Estrous cycle</subject><subject>Estrous Cycle - drug effects</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genistein</subject><subject>Genistein - pharmacology</subject><subject>Hormones and behavior</subject><subject>Isoflavones - pharmacology</subject><subject>Lordosis</subject><subject>Ovary - drug effects</subject><subject>Phytoestrogen</subject><subject>Phytoestrogens</subject><subject>Plant Preparations</subject><subject>Plants</subject><subject>Posture - physiology</subject><subject>Psychology. Psychoanalysis. Psychiatry</subject><subject>Psychology. Psychophysiology</subject><subject>Rat</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sex Characteristics</subject><subject>Sex Differentiation - drug effects</subject><subject>Sexual Behavior, Animal - drug effects</subject><subject>Sexual differentiation</subject><issn>0018-506X</issn><issn>1095-6867</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkt9uFCEUxidGY9fqI2i40WjSURgGGPSiMU39kzTxQk28IywcLGYWtsDYru_h-8rsrm30pjeQQ37n8PF9NM1jgl8STPirzxiToWWYf3uO6YtadF1L7zQLgiVr-cDF3WZxjRw0D3L-UUvC-v5-c0Ao6WQ_8EXz-9Q5MCWj6FCAGHTRIyoJdFlBKOjSl3O0Pt-UCLmk-B1CPkJ19bmAD0gHi6z29lctjlAMKMMVsr6OTBAMoIo4WOkRUNIFLZOeD6Zgio_hNdqOnDIyG1OJedYYk43Z54fNPafHDI_2-2Hz9d3pl5MP7dmn9x9P3p61phd9aQ0bHDBBln0vLZcapJOmt8se8EAJx4JZTjUXjlDBJGjKpHCYC00wlR1m9LB5tpu7TvFiqnrUymcD46irF1NWgnImaI9vBYkkfOgkqSDbgSbFnBM4tU5-pdNGEazm4NQ2ODWnojBV2-AUrX1P9hdMyxXYm659UhV4ugd0Nnp0SQfj8w3HCOWdGCr35j8Bxhc9G16q_eO1jPxXxtW_Mo533VBd_-khqWz8HKX1qX4TZaO_5SF_AA_Iyeo</recordid><startdate>20030801</startdate><enddate>20030801</enddate><creator>Kouki, Tom</creator><creator>Kishitake, Miki</creator><creator>Okamoto, Miho</creator><creator>Oosuka, Izumi</creator><creator>Takebe, Minoru</creator><creator>Yamanouchi, Korehito</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7X8</scope></search><sort><creationdate>20030801</creationdate><title>Effects of neonatal treatment with phytoestrogens, genistein and daidzein, on sex difference in female rat brain function: estrous cycle and lordosis</title><author>Kouki, Tom ; Kishitake, Miki ; Okamoto, Miho ; Oosuka, Izumi ; Takebe, Minoru ; Yamanouchi, Korehito</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-c58fe571b449d69ae9f9c4db4e08316075d63a67f13759ea3597f067a10392053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Behavioral psychophysiology</topic><topic>Biological and medical sciences</topic><topic>Brain</topic><topic>daidzein</topic><topic>Estradiol - physiology</topic><topic>Estrogens, Non-Steroidal - pharmacology</topic><topic>Estrous cycle</topic><topic>Estrous Cycle - drug effects</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genistein</topic><topic>Genistein - pharmacology</topic><topic>Hormones and behavior</topic><topic>Isoflavones - pharmacology</topic><topic>Lordosis</topic><topic>Ovary - drug effects</topic><topic>Phytoestrogen</topic><topic>Phytoestrogens</topic><topic>Plant Preparations</topic><topic>Plants</topic><topic>Posture - physiology</topic><topic>Psychology. Psychoanalysis. Psychiatry</topic><topic>Psychology. Psychophysiology</topic><topic>Rat</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Sex Characteristics</topic><topic>Sex Differentiation - drug effects</topic><topic>Sexual Behavior, Animal - drug effects</topic><topic>Sexual differentiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kouki, Tom</creatorcontrib><creatorcontrib>Kishitake, Miki</creatorcontrib><creatorcontrib>Okamoto, Miho</creatorcontrib><creatorcontrib>Oosuka, Izumi</creatorcontrib><creatorcontrib>Takebe, Minoru</creatorcontrib><creatorcontrib>Yamanouchi, Korehito</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Hormones and behavior</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kouki, Tom</au><au>Kishitake, Miki</au><au>Okamoto, Miho</au><au>Oosuka, Izumi</au><au>Takebe, Minoru</au><au>Yamanouchi, Korehito</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of neonatal treatment with phytoestrogens, genistein and daidzein, on sex difference in female rat brain function: estrous cycle and lordosis</atitle><jtitle>Hormones and behavior</jtitle><addtitle>Horm Behav</addtitle><date>2003-08-01</date><risdate>2003</risdate><volume>44</volume><issue>2</issue><spage>140</spage><epage>145</epage><pages>140-145</pages><artnum>140</artnum><issn>0018-506X</issn><eissn>1095-6867</eissn><coden>HOBEAO</coden><abstract>It is well known that neonatal exposure to estrogen induces masculinization or defeminization of the brain. In this study, the effects of neonatal treatment with two kinds of soybean isoflavone aglycone, genistein (GS) and daidzein (DZ), on the estrous cycle and lordosis behavior were investigated. Female rats were injected subcutaneously with 1 mg GS, 1 mg DZ, 100 μg estradiol (E 2), or oil daily for 5 days from birth. As a result, vaginal opening was advanced in GS- or E 2-treated females. A vaginal smear check indicated that oil- or DZ-treated females showed a constant 4- or 5-day estrous cycle, whereas GS- or E 2-treated rats showed a persistent or prolonged estrus. Ovariectomy was performed in all females at 60 days of age. The ovaries in the GS- or E 2-treated groups were smaller than those in the oil- and DZ-treated groups and contained no corpora lutea. In the DZ group, although corpora lutea were seen, ovaries were smaller than that of control females. Behavioral tests were carried out after implantation of E 2-tubes. All of the oil- or DZ-treated females showed lordosis with a high lordosis quotient (LQ). On the other hand, as male rats, LQs were extremely low in the E 2-treated group, when compared to the oil-treated group. In the GS-treated group, the mean LQ was lower than that in the oil-treated group, but higher than those in the E 2-treated female or male groups. These results suggest that genistein acts as an estrogen in the sexual differentiation of the brain and causes defeminization of the brain in regulating lordosis and the estrous cycle in rats. In addition, neonatal daidzein also has some influence on ovarian function.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>13129486</pmid><doi>10.1016/S0018-506X(03)00122-3</doi><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0018-506X
ispartof Hormones and behavior, 2003-08, Vol.44 (2), p.140-145, Article 140
issn 0018-506X
1095-6867
language eng
recordid cdi_proquest_miscellaneous_73657340
source MEDLINE; Elsevier ScienceDirect Journals Complete
subjects Animals
Animals, Newborn
Behavioral psychophysiology
Biological and medical sciences
Brain
daidzein
Estradiol - physiology
Estrogens, Non-Steroidal - pharmacology
Estrous cycle
Estrous Cycle - drug effects
Female
Fundamental and applied biological sciences. Psychology
Genistein
Genistein - pharmacology
Hormones and behavior
Isoflavones - pharmacology
Lordosis
Ovary - drug effects
Phytoestrogen
Phytoestrogens
Plant Preparations
Plants
Posture - physiology
Psychology. Psychoanalysis. Psychiatry
Psychology. Psychophysiology
Rat
Rats
Rats, Wistar
Sex Characteristics
Sex Differentiation - drug effects
Sexual Behavior, Animal - drug effects
Sexual differentiation
title Effects of neonatal treatment with phytoestrogens, genistein and daidzein, on sex difference in female rat brain function: estrous cycle and lordosis
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T11%3A44%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20neonatal%20treatment%20with%20phytoestrogens,%20genistein%20and%20daidzein,%20on%20sex%20difference%20in%20female%20rat%20brain%20function:%20estrous%20cycle%20and%20lordosis&rft.jtitle=Hormones%20and%20behavior&rft.au=Kouki,%20Tom&rft.date=2003-08-01&rft.volume=44&rft.issue=2&rft.spage=140&rft.epage=145&rft.pages=140-145&rft.artnum=140&rft.issn=0018-506X&rft.eissn=1095-6867&rft.coden=HOBEAO&rft_id=info:doi/10.1016/S0018-506X(03)00122-3&rft_dat=%3Cproquest_cross%3E19168291%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19168291&rft_id=info:pmid/13129486&rft_els_id=S0018506X03001223&rfr_iscdi=true