Behavior of Mycoplasma hominis in a Human Diploid Cell Culture System
The behavior of Mycoplasma hominis in normal human embryonic lung fibroblast (HAIN‐55) cell cultures was investigated. Multiplication patterns of cell‐associated mycoplasmas and of extracellular mycoplasmas in the HAIN‐55 cultures depended upon the size of the inoculum. This relationship did not var...
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| Veröffentlicht in: | Microbiology and immunology 1981-06, Vol.25 (6), p.537-543 |
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| container_issue | 6 |
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| container_title | Microbiology and immunology |
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| creator | Sasaki, Tsuguo Shintani, Miharu Yamada, Kenichiro Okumura, Hideo Kihara, Kojo |
| description | The behavior of Mycoplasma hominis in normal human embryonic lung fibroblast (HAIN‐55) cell cultures was investigated. Multiplication patterns of cell‐associated mycoplasmas and of extracellular mycoplasmas in the HAIN‐55 cultures depended upon the size of the inoculum. This relationship did not vary with the number of days the cells had been cultured, nor with the number of HAIN‐55 cell passages. The maximum mycoplasmal growth was obtained with inoculum sizes of 105 to 106 colony‐forming units (CFU)/ml. The recovery of mycoplasmas decreased rapidly with inoculum size beyond 107 CFU/ml, and growth of the HAIN‐55 cells was inhibited. Growth of the cells was also inhibited by the addition of the cytoplasmic fraction of Mycoplasma hominis. |
| doi_str_mv | 10.1111/j.1348-0421.1981.tb00055.x |
| format | Article |
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Multiplication patterns of cell‐associated mycoplasmas and of extracellular mycoplasmas in the HAIN‐55 cultures depended upon the size of the inoculum. This relationship did not vary with the number of days the cells had been cultured, nor with the number of HAIN‐55 cell passages. The maximum mycoplasmal growth was obtained with inoculum sizes of 105 to 106 colony‐forming units (CFU)/ml. The recovery of mycoplasmas decreased rapidly with inoculum size beyond 107 CFU/ml, and growth of the HAIN‐55 cells was inhibited. Growth of the cells was also inhibited by the addition of the cytoplasmic fraction of Mycoplasma hominis.</description><identifier>ISSN: 0385-5600</identifier><identifier>EISSN: 1348-0421</identifier><identifier>DOI: 10.1111/j.1348-0421.1981.tb00055.x</identifier><identifier>PMID: 7278706</identifier><language>eng</language><publisher>Australia: Blackwell Publishing Ltd</publisher><subject>Cell Line ; Cell Membrane ; Cells, Cultured - microbiology ; Culture Media ; Cytoplasm ; Fibroblasts ; Humans ; Kinetics ; Mycoplasma - growth & development ; Mycoplasma - ultrastructure ; Mycoplasma hominis</subject><ispartof>Microbiology and immunology, 1981-06, Vol.25 (6), p.537-543</ispartof><rights>owned by Center for Academic Publications Japan (Publisher)</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4985-489e62d2800642d559177278cd15fe27d2b62a96ea2d3b6f32052c255c46042f3</citedby><cites>FETCH-LOGICAL-c4985-489e62d2800642d559177278cd15fe27d2b62a96ea2d3b6f32052c255c46042f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7278706$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sasaki, Tsuguo</creatorcontrib><creatorcontrib>Shintani, Miharu</creatorcontrib><creatorcontrib>Yamada, Kenichiro</creatorcontrib><creatorcontrib>Okumura, Hideo</creatorcontrib><creatorcontrib>Kihara, Kojo</creatorcontrib><title>Behavior of Mycoplasma hominis in a Human Diploid Cell Culture System</title><title>Microbiology and immunology</title><addtitle>Microbiology and Immunology</addtitle><description>The behavior of Mycoplasma hominis in normal human embryonic lung fibroblast (HAIN‐55) cell cultures was investigated. Multiplication patterns of cell‐associated mycoplasmas and of extracellular mycoplasmas in the HAIN‐55 cultures depended upon the size of the inoculum. This relationship did not vary with the number of days the cells had been cultured, nor with the number of HAIN‐55 cell passages. The maximum mycoplasmal growth was obtained with inoculum sizes of 105 to 106 colony‐forming units (CFU)/ml. The recovery of mycoplasmas decreased rapidly with inoculum size beyond 107 CFU/ml, and growth of the HAIN‐55 cells was inhibited. Growth of the cells was also inhibited by the addition of the cytoplasmic fraction of Mycoplasma hominis.</description><subject>Cell Line</subject><subject>Cell Membrane</subject><subject>Cells, Cultured - microbiology</subject><subject>Culture Media</subject><subject>Cytoplasm</subject><subject>Fibroblasts</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Mycoplasma - growth & development</subject><subject>Mycoplasma - ultrastructure</subject><subject>Mycoplasma hominis</subject><issn>0385-5600</issn><issn>1348-0421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVUctOwzAQtBAIyuMTkCwOiEuCvX4lnIDylCgcCuJouYkjXJKmxA20f49Dqx5B7MUrzex4dgehI0piGup0HFPGk4hwoDFNExrPRoQQIeL5BuqtoU3UIywRkZCE7KBd78eEgIKEb6NtBSpRRPbQ9aV9M5-ubnBd4MEiq6el8ZXBb3XlJs5jN8EG37WVmeArNy1rl-O-LUvcb8tZ21g8XPiZrfbRVmFKbw9W7x56ubl-7t9FD0-39_2LhyjjaXDCk9RKyCEhRHLIhUip6pxkORWFBZXDSIJJpTWQs5EsGBABGQiRcRk2KtgeOl7qTpv6o7V-pivns-DHTGzdeq2YDNsy-JNIBVeKCR6IJ78TeQqSc0FVoJ4tqVlTe9_YQk8bV5lmoSnRXS56rLvj6-74ustFr3LR8zB8uPqnHVU2X4-uggj4-RL_cqVd_ENZD-4HP22QiJYSLkQyX0uY5l1LxZTQr4-3Wgyv6FBR0MC-AU9AqV4</recordid><startdate>198106</startdate><enddate>198106</enddate><creator>Sasaki, Tsuguo</creator><creator>Shintani, Miharu</creator><creator>Yamada, Kenichiro</creator><creator>Okumura, Hideo</creator><creator>Kihara, Kojo</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>198106</creationdate><title>Behavior of Mycoplasma hominis in a Human Diploid Cell Culture System</title><author>Sasaki, Tsuguo ; Shintani, Miharu ; Yamada, Kenichiro ; Okumura, Hideo ; Kihara, Kojo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4985-489e62d2800642d559177278cd15fe27d2b62a96ea2d3b6f32052c255c46042f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Cell Line</topic><topic>Cell Membrane</topic><topic>Cells, Cultured - microbiology</topic><topic>Culture Media</topic><topic>Cytoplasm</topic><topic>Fibroblasts</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Mycoplasma - growth & development</topic><topic>Mycoplasma - ultrastructure</topic><topic>Mycoplasma hominis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sasaki, Tsuguo</creatorcontrib><creatorcontrib>Shintani, Miharu</creatorcontrib><creatorcontrib>Yamada, Kenichiro</creatorcontrib><creatorcontrib>Okumura, Hideo</creatorcontrib><creatorcontrib>Kihara, Kojo</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiology and immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sasaki, Tsuguo</au><au>Shintani, Miharu</au><au>Yamada, Kenichiro</au><au>Okumura, Hideo</au><au>Kihara, Kojo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Behavior of Mycoplasma hominis in a Human Diploid Cell Culture System</atitle><jtitle>Microbiology and immunology</jtitle><addtitle>Microbiology and Immunology</addtitle><date>1981-06</date><risdate>1981</risdate><volume>25</volume><issue>6</issue><spage>537</spage><epage>543</epage><pages>537-543</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><abstract>The behavior of Mycoplasma hominis in normal human embryonic lung fibroblast (HAIN‐55) cell cultures was investigated. Multiplication patterns of cell‐associated mycoplasmas and of extracellular mycoplasmas in the HAIN‐55 cultures depended upon the size of the inoculum. This relationship did not vary with the number of days the cells had been cultured, nor with the number of HAIN‐55 cell passages. The maximum mycoplasmal growth was obtained with inoculum sizes of 105 to 106 colony‐forming units (CFU)/ml. The recovery of mycoplasmas decreased rapidly with inoculum size beyond 107 CFU/ml, and growth of the HAIN‐55 cells was inhibited. Growth of the cells was also inhibited by the addition of the cytoplasmic fraction of Mycoplasma hominis.</abstract><cop>Australia</cop><pub>Blackwell Publishing Ltd</pub><pmid>7278706</pmid><doi>10.1111/j.1348-0421.1981.tb00055.x</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Microbiology and immunology, 1981-06, Vol.25 (6), p.537-543 |
| issn | 0385-5600 1348-0421 |
| language | eng |
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| source | J-STAGE Free; MEDLINE; Open Access Titles of Japan; Alma/SFX Local Collection |
| subjects | Cell Line Cell Membrane Cells, Cultured - microbiology Culture Media Cytoplasm Fibroblasts Humans Kinetics Mycoplasma - growth & development Mycoplasma - ultrastructure Mycoplasma hominis |
| title | Behavior of Mycoplasma hominis in a Human Diploid Cell Culture System |
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