Bcl-2 prevents abnormal mitochondrial proliferation during etoposide-induced apoptosis
At the late stage of etoposide-induced apoptosis in HL-60 cells, marked by condensation of chromatin, mitochondria increase in numbers. There is also a drastic increase in mitochondrial DNA content. This increase in mitochondrial numbers and DNA content is an indicator of mitochondrial proliferation...
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| Veröffentlicht in: | Experimental cell research 2003-10, Vol.289 (2), p.275-281 |
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| creator | Eliseev, Roman A Gunter, Karlene K Gunter, Thomas E |
| description | At the late stage of etoposide-induced apoptosis in HL-60 cells, marked by condensation of chromatin, mitochondria increase in numbers. There is also a drastic increase in mitochondrial DNA content. This increase in mitochondrial numbers and DNA content is an indicator of mitochondrial proliferation during apoptosis. These proliferating mitochondria exhibit abnormal morphology and are impaired, which is demonstrated by decrease in mitochondrial membrane potential and ATP content. The described apoptosis-induced abnormal mitochondrial proliferation was inhibited by overexpression of Bcl-2 protein, which also diminishes mitochondrial impairment. The increase in mitochondrial DNA levels correlated with elevated expression of one of the regulators of mitochondrial DNA replication, mtSSB. Our data suggest that proliferation of mitochondria may be an integral part of a cascade of apoptotic events. |
| doi_str_mv | 10.1016/S0014-4827(03)00278-7 |
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There is also a drastic increase in mitochondrial DNA content. This increase in mitochondrial numbers and DNA content is an indicator of mitochondrial proliferation during apoptosis. These proliferating mitochondria exhibit abnormal morphology and are impaired, which is demonstrated by decrease in mitochondrial membrane potential and ATP content. The described apoptosis-induced abnormal mitochondrial proliferation was inhibited by overexpression of Bcl-2 protein, which also diminishes mitochondrial impairment. The increase in mitochondrial DNA levels correlated with elevated expression of one of the regulators of mitochondrial DNA replication, mtSSB. 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| subjects | Active Transport, Cell Nucleus - drug effects Active Transport, Cell Nucleus - genetics Adenosine Triphosphate - metabolism Apoptosis - drug effects Apoptosis - genetics DNA, Mitochondrial - drug effects DNA, Mitochondrial - genetics DNA-Binding Proteins - drug effects DNA-Binding Proteins - metabolism Etoposide - pharmacology HL-60 Cells Humans Membrane Potentials - drug effects Membrane Potentials - genetics Mitochondria - drug effects Mitochondria - genetics Mitochondria - pathology Proto-Oncogene Proteins c-bcl-2 - drug effects Proto-Oncogene Proteins c-bcl-2 - metabolism Signal Transduction - drug effects Signal Transduction - genetics Up-Regulation - drug effects Up-Regulation - genetics |
| title | Bcl-2 prevents abnormal mitochondrial proliferation during etoposide-induced apoptosis |
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