Methodology for predicting the separation of proteins by hydrophobic interaction chromatography and its application to a cell extract

Hydrophobic interaction chromatography (HIC) is widely used in the downstream processing of proteins. Resolution of HIC is very good, but sometimes not as high as expected. Resolution values could be increased if good operating conditions were selected. In this paper we present a methodology for sel...

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Veröffentlicht in:	Journal of Chromatography A 2003-08, Vol.1009 (1), p.189-196
Hauptverfasser:	Lienqueo, M.Elena, Mahn, Andrea, Vásquez, Liliana, Asenjo, Juan A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Cell Extracts - chemistry Chromatography, Liquid - methods Electrophoresis, Polyacrylamide Gel Fermentation Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Others Proteins Proteins - isolation & purification Saccharomyces cerevisiae - genetics Superoxide Dismutase - metabolism Various methods and equipments
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