INDUCTION OF NEUTRALIZING ANTIBODIES AND PARTIAL PROTECTION FROM VIRAL CHALLENGE IN MACACA FASCICULARIS IMMUNIZED WITH RECOMBINANT DENGUE 4 VIRUS ENVELOPE GLYCOPROTEIN EXPRESSED IN PICHIA PASTORIS
A recombinant vaccine that expresses the envelope (E) gene of dengue virus type 4 was tested for immunogenicity and protection in Macaca fascicularis. One hundred micrograms of semipurified recombinant E protein (E4rec) expressed in Pichia pastoris was used to immunize three animals. Neutralizing an...
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| Veröffentlicht in: | The American journal of tropical medicine and hygiene 2003-08, Vol.69 (2), p.129-134 |
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| creator | GUZMAN, MARIA G RODRIGUEZ, RAYNER RODRIGUEZ, ROSMARI HERMIDA, LISSET ALVAREZ, MAYLING LAZO, LAURA MUNE, MAYRA ROSARIO, DELFINA VALDES, KATIA VAZQUEZ, SUSANA MARTINEZ, RAFAEL SERRANO, TERESITA PAEZ, JORGE ESPINOSA, RAUL PUMARIEGA, TANIA GUILLEN, GERARDO |
| description | A recombinant vaccine that expresses the envelope (E) gene of dengue virus type 4 was tested for immunogenicity and protection in Macaca fascicularis. One hundred micrograms of semipurified recombinant E protein (E4rec) expressed in Pichia pastoris was used to immunize three animals. Neutralizing antibodies to dengue 4 virus with a titer of 1:30 were detected in all immunized monkeys prior to challenge. Animals were challenged with 10(5) plaque-forming units of dengue 4 virus. One vaccine-immunized monkey was protected from viremia, while the other two were partially protected. Monkeys immunized with E4rec elicited the highest neutralizing antibody titers (P < 0.05) ranging from 1:85 to 1:640 at day 30. In both immunized and control animals, the longest duration of viremia correlated with earliest and highest level of IgM antibody to dengue virus. The vaccinated animals showed anamnestic antibody responses upon virus challenge, indicating successful priming by the recombinant vaccine. Our results suggest that E4rec expressed in P. pastoris can provide partial protection against viremia. However, the results were not effective enough to use it as a vaccine candidate. Further work is required to improve the quality of the immunogen. |
| doi_str_mv | 10.4269/ajtmh.2003.69.129 |
| format | Article |
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One hundred micrograms of semipurified recombinant E protein (E4rec) expressed in Pichia pastoris was used to immunize three animals. Neutralizing antibodies to dengue 4 virus with a titer of 1:30 were detected in all immunized monkeys prior to challenge. Animals were challenged with 10(5) plaque-forming units of dengue 4 virus. One vaccine-immunized monkey was protected from viremia, while the other two were partially protected. Monkeys immunized with E4rec elicited the highest neutralizing antibody titers (P < 0.05) ranging from 1:85 to 1:640 at day 30. In both immunized and control animals, the longest duration of viremia correlated with earliest and highest level of IgM antibody to dengue virus. The vaccinated animals showed anamnestic antibody responses upon virus challenge, indicating successful priming by the recombinant vaccine. Our results suggest that E4rec expressed in P. pastoris can provide partial protection against viremia. However, the results were not effective enough to use it as a vaccine candidate. Further work is required to improve the quality of the immunogen.</description><identifier>ISSN: 0002-9637</identifier><identifier>EISSN: 1476-1645</identifier><identifier>DOI: 10.4269/ajtmh.2003.69.129</identifier><identifier>PMID: 13677367</identifier><identifier>CODEN: AJTHAB</identifier><language>eng</language><publisher>Lawrence, KS: ASTMH</publisher><subject>Animals ; Antibodies, Viral - biosynthesis ; Antibodies, Viral - blood ; Arboviroses ; Biological and medical sciences ; Dengue fevers ; Dengue Virus - immunology ; Dengue virus type 4 ; Disease Models, Animal ; Human viral diseases ; Infectious diseases ; Macaca fascicularis ; Male ; Medical sciences ; Neutralization Tests ; Pichia ; Pichia pastoris ; Severe Dengue - immunology ; Severe Dengue - prevention & control ; Tropical medicine ; Tropical viral diseases ; Vaccines, Synthetic - immunology ; Viral diseases ; Viral Envelope Proteins - immunology ; Viral Vaccines - immunology</subject><ispartof>The American journal of tropical medicine and hygiene, 2003-08, Vol.69 (2), p.129-134</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-435fc61d87a76ad9c754c65535f68db18402773aaab1e5376410cc4a5dcb78213</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15091276$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/13677367$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GUZMAN, MARIA G</creatorcontrib><creatorcontrib>RODRIGUEZ, RAYNER</creatorcontrib><creatorcontrib>RODRIGUEZ, ROSMARI</creatorcontrib><creatorcontrib>HERMIDA, LISSET</creatorcontrib><creatorcontrib>ALVAREZ, MAYLING</creatorcontrib><creatorcontrib>LAZO, LAURA</creatorcontrib><creatorcontrib>MUNE, MAYRA</creatorcontrib><creatorcontrib>ROSARIO, DELFINA</creatorcontrib><creatorcontrib>VALDES, KATIA</creatorcontrib><creatorcontrib>VAZQUEZ, SUSANA</creatorcontrib><creatorcontrib>MARTINEZ, RAFAEL</creatorcontrib><creatorcontrib>SERRANO, TERESITA</creatorcontrib><creatorcontrib>PAEZ, JORGE</creatorcontrib><creatorcontrib>ESPINOSA, RAUL</creatorcontrib><creatorcontrib>PUMARIEGA, TANIA</creatorcontrib><creatorcontrib>GUILLEN, GERARDO</creatorcontrib><title>INDUCTION OF NEUTRALIZING ANTIBODIES AND PARTIAL PROTECTION FROM VIRAL CHALLENGE IN MACACA FASCICULARIS IMMUNIZED WITH RECOMBINANT DENGUE 4 VIRUS ENVELOPE GLYCOPROTEIN EXPRESSED IN PICHIA PASTORIS</title><title>The American journal of tropical medicine and hygiene</title><addtitle>Am J Trop Med Hyg</addtitle><description>A recombinant vaccine that expresses the envelope (E) gene of dengue virus type 4 was tested for immunogenicity and protection in Macaca fascicularis. One hundred micrograms of semipurified recombinant E protein (E4rec) expressed in Pichia pastoris was used to immunize three animals. Neutralizing antibodies to dengue 4 virus with a titer of 1:30 were detected in all immunized monkeys prior to challenge. Animals were challenged with 10(5) plaque-forming units of dengue 4 virus. One vaccine-immunized monkey was protected from viremia, while the other two were partially protected. Monkeys immunized with E4rec elicited the highest neutralizing antibody titers (P < 0.05) ranging from 1:85 to 1:640 at day 30. In both immunized and control animals, the longest duration of viremia correlated with earliest and highest level of IgM antibody to dengue virus. The vaccinated animals showed anamnestic antibody responses upon virus challenge, indicating successful priming by the recombinant vaccine. Our results suggest that E4rec expressed in P. pastoris can provide partial protection against viremia. However, the results were not effective enough to use it as a vaccine candidate. Further work is required to improve the quality of the immunogen.</description><subject>Animals</subject><subject>Antibodies, Viral - biosynthesis</subject><subject>Antibodies, Viral - blood</subject><subject>Arboviroses</subject><subject>Biological and medical sciences</subject><subject>Dengue fevers</subject><subject>Dengue Virus - immunology</subject><subject>Dengue virus type 4</subject><subject>Disease Models, Animal</subject><subject>Human viral diseases</subject><subject>Infectious diseases</subject><subject>Macaca fascicularis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Neutralization Tests</subject><subject>Pichia</subject><subject>Pichia pastoris</subject><subject>Severe Dengue - immunology</subject><subject>Severe Dengue - prevention & control</subject><subject>Tropical medicine</subject><subject>Tropical viral diseases</subject><subject>Vaccines, Synthetic - immunology</subject><subject>Viral diseases</subject><subject>Viral Envelope Proteins - immunology</subject><subject>Viral Vaccines - immunology</subject><issn>0002-9637</issn><issn>1476-1645</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUUlu2zAUFYoWjZP2AN0U3DQ7uaREkdJSkWmbgCZoSNtsBJqSawdynIg2jN6vB-v3AHhZEAT5iTcQ71nWF4LH1GHBd_W826zGDsbumAVj4gTvrBGhnNmEUe-9NcIYO3bAXH5j3RrzjDHxHUI-WjfEZZzDHll_ZTqpo0pmKcqmKBV1VYSxfJLpDIVpJR-yiRQlXCcoD4tKhjHKi6wSZ8a0yBL0KIGBonkYxyKdCSRTlIQRLDQNy0hGdRwWskQySepUPokJ-iGrOSpElCUPMgUTNAFeLRA9StUlEumjiLNcoFn8K8pOdqApfuaFKEvgw5DLaC5D-FJZZSD-yfqwVL3pPl_OO6ueiiqa23E2k1EY25q6ZGdT11tqRlqfK85UG2juUc08D56Z3y6IT7EDsSilFqTzXM4owVpT5bV6wSE59866P-u-Dtu3fWd2zWZtdNf36qXb7k0DkVLfd_h_gYQHxPEoBiA5A_WwNWbols3rsN6o4U9DcHPsuDl13Bw7bmCCjoHz9SK-X2y69sq4lAqAbxeAMlr1y0G96LW54jwM7pxdcav179VhPXSN2ai-B1nSHA4HsHNOhv8AfZquRQ</recordid><startdate>20030801</startdate><enddate>20030801</enddate><creator>GUZMAN, MARIA G</creator><creator>RODRIGUEZ, RAYNER</creator><creator>RODRIGUEZ, ROSMARI</creator><creator>HERMIDA, LISSET</creator><creator>ALVAREZ, MAYLING</creator><creator>LAZO, LAURA</creator><creator>MUNE, MAYRA</creator><creator>ROSARIO, DELFINA</creator><creator>VALDES, KATIA</creator><creator>VAZQUEZ, SUSANA</creator><creator>MARTINEZ, RAFAEL</creator><creator>SERRANO, TERESITA</creator><creator>PAEZ, JORGE</creator><creator>ESPINOSA, RAUL</creator><creator>PUMARIEGA, TANIA</creator><creator>GUILLEN, GERARDO</creator><general>ASTMH</general><general>Allen Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20030801</creationdate><title>INDUCTION OF NEUTRALIZING ANTIBODIES AND PARTIAL PROTECTION FROM VIRAL CHALLENGE IN MACACA FASCICULARIS IMMUNIZED WITH RECOMBINANT DENGUE 4 VIRUS ENVELOPE GLYCOPROTEIN EXPRESSED IN PICHIA PASTORIS</title><author>GUZMAN, MARIA G ; RODRIGUEZ, RAYNER ; RODRIGUEZ, ROSMARI ; HERMIDA, LISSET ; ALVAREZ, MAYLING ; LAZO, LAURA ; MUNE, MAYRA ; ROSARIO, DELFINA ; VALDES, KATIA ; VAZQUEZ, SUSANA ; MARTINEZ, RAFAEL ; SERRANO, TERESITA ; PAEZ, JORGE ; ESPINOSA, RAUL ; PUMARIEGA, TANIA ; GUILLEN, GERARDO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-435fc61d87a76ad9c754c65535f68db18402773aaab1e5376410cc4a5dcb78213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Antibodies, Viral - biosynthesis</topic><topic>Antibodies, Viral - blood</topic><topic>Arboviroses</topic><topic>Biological and medical sciences</topic><topic>Dengue fevers</topic><topic>Dengue Virus - immunology</topic><topic>Dengue virus type 4</topic><topic>Disease Models, Animal</topic><topic>Human viral diseases</topic><topic>Infectious diseases</topic><topic>Macaca fascicularis</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Neutralization Tests</topic><topic>Pichia</topic><topic>Pichia pastoris</topic><topic>Severe Dengue - immunology</topic><topic>Severe Dengue - prevention & control</topic><topic>Tropical medicine</topic><topic>Tropical viral diseases</topic><topic>Vaccines, Synthetic - immunology</topic><topic>Viral diseases</topic><topic>Viral Envelope Proteins - immunology</topic><topic>Viral Vaccines - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GUZMAN, MARIA G</creatorcontrib><creatorcontrib>RODRIGUEZ, RAYNER</creatorcontrib><creatorcontrib>RODRIGUEZ, ROSMARI</creatorcontrib><creatorcontrib>HERMIDA, LISSET</creatorcontrib><creatorcontrib>ALVAREZ, MAYLING</creatorcontrib><creatorcontrib>LAZO, LAURA</creatorcontrib><creatorcontrib>MUNE, MAYRA</creatorcontrib><creatorcontrib>ROSARIO, DELFINA</creatorcontrib><creatorcontrib>VALDES, KATIA</creatorcontrib><creatorcontrib>VAZQUEZ, SUSANA</creatorcontrib><creatorcontrib>MARTINEZ, RAFAEL</creatorcontrib><creatorcontrib>SERRANO, TERESITA</creatorcontrib><creatorcontrib>PAEZ, JORGE</creatorcontrib><creatorcontrib>ESPINOSA, RAUL</creatorcontrib><creatorcontrib>PUMARIEGA, TANIA</creatorcontrib><creatorcontrib>GUILLEN, GERARDO</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The American journal of tropical medicine and hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GUZMAN, MARIA G</au><au>RODRIGUEZ, RAYNER</au><au>RODRIGUEZ, ROSMARI</au><au>HERMIDA, LISSET</au><au>ALVAREZ, MAYLING</au><au>LAZO, LAURA</au><au>MUNE, MAYRA</au><au>ROSARIO, DELFINA</au><au>VALDES, KATIA</au><au>VAZQUEZ, SUSANA</au><au>MARTINEZ, RAFAEL</au><au>SERRANO, TERESITA</au><au>PAEZ, JORGE</au><au>ESPINOSA, RAUL</au><au>PUMARIEGA, TANIA</au><au>GUILLEN, GERARDO</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>INDUCTION OF NEUTRALIZING ANTIBODIES AND PARTIAL PROTECTION FROM VIRAL CHALLENGE IN MACACA FASCICULARIS IMMUNIZED WITH RECOMBINANT DENGUE 4 VIRUS ENVELOPE GLYCOPROTEIN EXPRESSED IN PICHIA PASTORIS</atitle><jtitle>The American journal of tropical medicine and hygiene</jtitle><addtitle>Am J Trop Med Hyg</addtitle><date>2003-08-01</date><risdate>2003</risdate><volume>69</volume><issue>2</issue><spage>129</spage><epage>134</epage><pages>129-134</pages><issn>0002-9637</issn><eissn>1476-1645</eissn><coden>AJTHAB</coden><abstract>A recombinant vaccine that expresses the envelope (E) gene of dengue virus type 4 was tested for immunogenicity and protection in Macaca fascicularis. One hundred micrograms of semipurified recombinant E protein (E4rec) expressed in Pichia pastoris was used to immunize three animals. Neutralizing antibodies to dengue 4 virus with a titer of 1:30 were detected in all immunized monkeys prior to challenge. Animals were challenged with 10(5) plaque-forming units of dengue 4 virus. One vaccine-immunized monkey was protected from viremia, while the other two were partially protected. Monkeys immunized with E4rec elicited the highest neutralizing antibody titers (P < 0.05) ranging from 1:85 to 1:640 at day 30. In both immunized and control animals, the longest duration of viremia correlated with earliest and highest level of IgM antibody to dengue virus. The vaccinated animals showed anamnestic antibody responses upon virus challenge, indicating successful priming by the recombinant vaccine. Our results suggest that E4rec expressed in P. pastoris can provide partial protection against viremia. However, the results were not effective enough to use it as a vaccine candidate. Further work is required to improve the quality of the immunogen.</abstract><cop>Lawrence, KS</cop><pub>ASTMH</pub><pmid>13677367</pmid><doi>10.4269/ajtmh.2003.69.129</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antibodies, Viral - biosynthesis Antibodies, Viral - blood Arboviroses Biological and medical sciences Dengue fevers Dengue Virus - immunology Dengue virus type 4 Disease Models, Animal Human viral diseases Infectious diseases Macaca fascicularis Male Medical sciences Neutralization Tests Pichia Pichia pastoris Severe Dengue - immunology Severe Dengue - prevention & control Tropical medicine Tropical viral diseases Vaccines, Synthetic - immunology Viral diseases Viral Envelope Proteins - immunology Viral Vaccines - immunology |
| title | INDUCTION OF NEUTRALIZING ANTIBODIES AND PARTIAL PROTECTION FROM VIRAL CHALLENGE IN MACACA FASCICULARIS IMMUNIZED WITH RECOMBINANT DENGUE 4 VIRUS ENVELOPE GLYCOPROTEIN EXPRESSED IN PICHIA PASTORIS |
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