An inducible Streptomyces gene cluster involved in aromatic compound metabolism
Streptomyces setonii (ATCC 39116) is a thermophilic soil actinomycete capable of degrading single aromatic compounds including phenol and benzoate via the ortho-cleavage pathway. Previously, a 6.3-kb S. setonii DNA fragment containing a thermophilic catechol 1,2-dioxygenase (C12O) gene was isolated...
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| Veröffentlicht in: | FEMS microbiology letters 2003-09, Vol.226 (1), p.151-157 |
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| description | Streptomyces setonii (ATCC 39116) is a thermophilic soil actinomycete capable of degrading single aromatic compounds including phenol and benzoate via the
ortho-cleavage pathway. Previously, a 6.3-kb
S. setonii DNA fragment containing a thermophilic catechol 1,2-dioxygenase (C12O) gene was isolated and functionally overexpressed in
Escherichia coli (An et al., FEMS Microbiol. Lett. 195 (2001) 17–22). Here the 6.3-kb
S. setonii DNA fragment was shown to be organized into two putative divergently transcribed gene clusters with six complete and one incomplete open reading frames (ORFs). The first cluster with three ORFs showed homologies to previously known
benA,
benB, and
benC, implying it is a part of the benzoate catabolic operon. The second cluster revealed an
ortho-cleavage catechol catabolic operon with three translationally coupled ORFs (in order):
catR, a putative LysR-type regulatory gene;
catB, a muconate cycloisomerase gene;
catA, a C12O gene. Each of these individually cloned ORFs was expressed in
E. coli and identified as a distinct protein. The expression of the cloned
S. setonii catechol operon was induced in
Streptomyces lividans by specific single aromatic compounds including catechol, phenol, and 4-chlorophenol. A similar induction pattern was also observed using a luciferase gene-fused reporter system. |
| doi_str_mv | 10.1016/S0378-1097(03)00585-8 |
| format | Article |
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ortho-cleavage pathway. Previously, a 6.3-kb
S. setonii DNA fragment containing a thermophilic catechol 1,2-dioxygenase (C12O) gene was isolated and functionally overexpressed in
Escherichia coli (An et al., FEMS Microbiol. Lett. 195 (2001) 17–22). Here the 6.3-kb
S. setonii DNA fragment was shown to be organized into two putative divergently transcribed gene clusters with six complete and one incomplete open reading frames (ORFs). The first cluster with three ORFs showed homologies to previously known
benA,
benB, and
benC, implying it is a part of the benzoate catabolic operon. The second cluster revealed an
ortho-cleavage catechol catabolic operon with three translationally coupled ORFs (in order):
catR, a putative LysR-type regulatory gene;
catB, a muconate cycloisomerase gene;
catA, a C12O gene. Each of these individually cloned ORFs was expressed in
E. coli and identified as a distinct protein. The expression of the cloned
S. setonii catechol operon was induced in
Streptomyces lividans by specific single aromatic compounds including catechol, phenol, and 4-chlorophenol. A similar induction pattern was also observed using a luciferase gene-fused reporter system.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1016/S0378-1097(03)00585-8</identifier><identifier>PMID: 13129621</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Elsevier B.V</publisher><subject>Antibiotics (antibacterial agents, antifungal agents) ; Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents ; Applied microbiology ; Bacteriology ; Base Sequence ; Benzoates - metabolism ; Biological and medical sciences ; Catechol 1,2-Dioxygenase ; Catechol metabolic gene cluster ; Catechols - metabolism ; Chlorophenols - metabolism ; Dioxygenases ; DNA, Bacterial - chemistry ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Bacterial ; Gene Order - genetics ; Genes, Bacterial ; Genes, Reporter ; Luciferases - genetics ; Luciferases - metabolism ; LysR-type regulation ; Metabolism. Enzymes ; Microbiology ; Molecular Sequence Data ; Multigene Family ; Operon ; Oxygenases - genetics ; Oxygenases - metabolism ; Parabens - metabolism ; Phenol - metabolism ; Sequence Homology ; Streptomyces ; Streptomyces - enzymology ; Streptomyces - genetics ; Transcription, Genetic</subject><ispartof>FEMS microbiology letters, 2003-09, Vol.226 (1), p.151-157</ispartof><rights>2003 Federation of European Microbiological Societies</rights><rights>2003 Federation of European Microbiological Societies 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5651-65624fbacbbd17884a042005672c24d1634d3932c36fd865d9450b2920acd9123</citedby><cites>FETCH-LOGICAL-c5651-65624fbacbbd17884a042005672c24d1634d3932c36fd865d9450b2920acd9123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0378-1097%2803%2900585-8$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2FS0378-1097%2803%2900585-8$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15491964$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/13129621$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Hyun-Joo</creatorcontrib><creatorcontrib>Kim, Eung-Soo</creatorcontrib><title>An inducible Streptomyces gene cluster involved in aromatic compound metabolism</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Streptomyces setonii (ATCC 39116) is a thermophilic soil actinomycete capable of degrading single aromatic compounds including phenol and benzoate via the
ortho-cleavage pathway. Previously, a 6.3-kb
S. setonii DNA fragment containing a thermophilic catechol 1,2-dioxygenase (C12O) gene was isolated and functionally overexpressed in
Escherichia coli (An et al., FEMS Microbiol. Lett. 195 (2001) 17–22). Here the 6.3-kb
S. setonii DNA fragment was shown to be organized into two putative divergently transcribed gene clusters with six complete and one incomplete open reading frames (ORFs). The first cluster with three ORFs showed homologies to previously known
benA,
benB, and
benC, implying it is a part of the benzoate catabolic operon. The second cluster revealed an
ortho-cleavage catechol catabolic operon with three translationally coupled ORFs (in order):
catR, a putative LysR-type regulatory gene;
catB, a muconate cycloisomerase gene;
catA, a C12O gene. Each of these individually cloned ORFs was expressed in
E. coli and identified as a distinct protein. The expression of the cloned
S. setonii catechol operon was induced in
Streptomyces lividans by specific single aromatic compounds including catechol, phenol, and 4-chlorophenol. A similar induction pattern was also observed using a luciferase gene-fused reporter system.</description><subject>Antibiotics (antibacterial agents, antifungal agents)</subject><subject>Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents</subject><subject>Applied microbiology</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Benzoates - metabolism</subject><subject>Biological and medical sciences</subject><subject>Catechol 1,2-Dioxygenase</subject><subject>Catechol metabolic gene cluster</subject><subject>Catechols - metabolism</subject><subject>Chlorophenols - metabolism</subject><subject>Dioxygenases</subject><subject>DNA, Bacterial - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Gene Order - genetics</subject><subject>Genes, Bacterial</subject><subject>Genes, Reporter</subject><subject>Luciferases - genetics</subject><subject>Luciferases - metabolism</subject><subject>LysR-type regulation</subject><subject>Metabolism. Enzymes</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Operon</subject><subject>Oxygenases - genetics</subject><subject>Oxygenases - metabolism</subject><subject>Parabens - metabolism</subject><subject>Phenol - metabolism</subject><subject>Sequence Homology</subject><subject>Streptomyces</subject><subject>Streptomyces - enzymology</subject><subject>Streptomyces - genetics</subject><subject>Transcription, Genetic</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU2L1TAUhoMoznX0JyjdKLqo5rvJSobBUeHKLEbXIU1OJZI2NWmv3H9vO704CMK4SbJ4nrwnbxB6TvBbgol8d4NZo2qCdfMaszcYCyVq9QDtiGh4LbVUD9HuD3KGnpTyA2PMKZaP0RlhhGpJyQ5dXwxVGPzsQhuhupkyjFPqjw5K9R0GqFycywR5YQ4pHsAvh8rm1NspuMqlfkzz4KseJtumGEr_FD3qbCzw7LSfo29XH75efqr31x8_X17sayekILUUkvKuta5tPWmU4nYdDQvZUEe5J5JxzzSjjsnOKym85gK3VFNsndeEsnP0art3zOnnDGUyfSgOYrQDpLmYhklOlcD3gkQpTZZlAcUGupxKydCZMYfe5qMh2KyVm9vKzdqnwczcVm5W78UpYG578HfWqeMFeHkCbHE2dtkOLpQ7TnBNtOQLpzfuV4hw_L90c_VlT8SagTc3zeO_zfovs17nfr8psHzTIUA2xQUYHPiQwU3Gp3DPy38DFoC7DQ</recordid><startdate>20030912</startdate><enddate>20030912</enddate><creator>Park, Hyun-Joo</creator><creator>Kim, Eung-Soo</creator><general>Elsevier B.V</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20030912</creationdate><title>An inducible Streptomyces gene cluster involved in aromatic compound metabolism</title><author>Park, Hyun-Joo ; Kim, Eung-Soo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5651-65624fbacbbd17884a042005672c24d1634d3932c36fd865d9450b2920acd9123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Antibiotics (antibacterial agents, antifungal agents)</topic><topic>Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents</topic><topic>Applied microbiology</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Benzoates - metabolism</topic><topic>Biological and medical sciences</topic><topic>Catechol 1,2-Dioxygenase</topic><topic>Catechol metabolic gene cluster</topic><topic>Catechols - metabolism</topic><topic>Chlorophenols - metabolism</topic><topic>Dioxygenases</topic><topic>DNA, Bacterial - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Gene Order - genetics</topic><topic>Genes, Bacterial</topic><topic>Genes, Reporter</topic><topic>Luciferases - genetics</topic><topic>Luciferases - metabolism</topic><topic>LysR-type regulation</topic><topic>Metabolism. Enzymes</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Operon</topic><topic>Oxygenases - genetics</topic><topic>Oxygenases - metabolism</topic><topic>Parabens - metabolism</topic><topic>Phenol - metabolism</topic><topic>Sequence Homology</topic><topic>Streptomyces</topic><topic>Streptomyces - enzymology</topic><topic>Streptomyces - genetics</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Hyun-Joo</creatorcontrib><creatorcontrib>Kim, Eung-Soo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Hyun-Joo</au><au>Kim, Eung-Soo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An inducible Streptomyces gene cluster involved in aromatic compound metabolism</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2003-09-12</date><risdate>2003</risdate><volume>226</volume><issue>1</issue><spage>151</spage><epage>157</epage><pages>151-157</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Streptomyces setonii (ATCC 39116) is a thermophilic soil actinomycete capable of degrading single aromatic compounds including phenol and benzoate via the
ortho-cleavage pathway. Previously, a 6.3-kb
S. setonii DNA fragment containing a thermophilic catechol 1,2-dioxygenase (C12O) gene was isolated and functionally overexpressed in
Escherichia coli (An et al., FEMS Microbiol. Lett. 195 (2001) 17–22). Here the 6.3-kb
S. setonii DNA fragment was shown to be organized into two putative divergently transcribed gene clusters with six complete and one incomplete open reading frames (ORFs). The first cluster with three ORFs showed homologies to previously known
benA,
benB, and
benC, implying it is a part of the benzoate catabolic operon. The second cluster revealed an
ortho-cleavage catechol catabolic operon with three translationally coupled ORFs (in order):
catR, a putative LysR-type regulatory gene;
catB, a muconate cycloisomerase gene;
catA, a C12O gene. Each of these individually cloned ORFs was expressed in
E. coli and identified as a distinct protein. The expression of the cloned
S. setonii catechol operon was induced in
Streptomyces lividans by specific single aromatic compounds including catechol, phenol, and 4-chlorophenol. A similar induction pattern was also observed using a luciferase gene-fused reporter system.</abstract><cop>Oxford, UK</cop><pub>Elsevier B.V</pub><pmid>13129621</pmid><doi>10.1016/S0378-1097(03)00585-8</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2003-09, Vol.226 (1), p.151-157 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73642850 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Antibiotics (antibacterial agents, antifungal agents) Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents Applied microbiology Bacteriology Base Sequence Benzoates - metabolism Biological and medical sciences Catechol 1,2-Dioxygenase Catechol metabolic gene cluster Catechols - metabolism Chlorophenols - metabolism Dioxygenases DNA, Bacterial - chemistry Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Gene Order - genetics Genes, Bacterial Genes, Reporter Luciferases - genetics Luciferases - metabolism LysR-type regulation Metabolism. Enzymes Microbiology Molecular Sequence Data Multigene Family Operon Oxygenases - genetics Oxygenases - metabolism Parabens - metabolism Phenol - metabolism Sequence Homology Streptomyces Streptomyces - enzymology Streptomyces - genetics Transcription, Genetic |
| title | An inducible Streptomyces gene cluster involved in aromatic compound metabolism |
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